SAP102 (DLG3) (NM_021120) Human Tagged ORF Clone

CAT#: RC210860

DLG3 (Myc-DDK-tagged)-Human discs, large homolog 3 (Drosophila) (DLG3), transcript variant 1

Product Images

Circular map for RC210860

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DLG3 (Cat# RC210860, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLG3 (Cat# TA502191). Positive lysates LY402837 (100ug) and LC402837 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY402837) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210860 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DLG3 protein (Cat# TP310860). The protein was produced from HEK293T cells transfected with DLG3 cDNA clone (Cat# RC210860) using MegaTran 2.0 (Cat# TT210002).

• 

  HEK293T cells transfected with either RC210860 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DLG3 antibody (TA502191), and then analyzed by flow cytometry.

Show all 5 images

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SAP102
• Synonyms: MRX; MRX90; NEDLG; PPP1R82; SAP102; XLMR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210860 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAAGCACCAGCACTGCTGTAAGTGCCCTGAGTGCTATGAGGTGACCCGCCTGGCCGCCCTGCGGC
  GCCTCGAGCCTCCGGGCTACGGCGACTGGCAAGTCCCCGACCCTTACGGGCCAGGTGGGGGCAACGGCGC
  CAGCGCGGGTTATGGGGGCTACAGCTCGCAGACCTTGCCCTCGCAGGCGGGGGCCACCCCCACCCCTCGC
  ACCAAGGCCAAGCTCATCCCCACCGGCCGGGATGTGGGGCCGGTGCCTCCTAAGCCAGTCCCGGGCAAGA
  GCACCCCCAAACTCAACGGCAGCGGCCCCAGCTGGTGGCCAGAGTGCACCTGTACCAACCGGGACTGGTA
  TGAGCAGGTGAATGGCAGTGATGGCATGTTCAAATATGAGGAAATCGTACTTGAGAGGGGCAACTCTGGC
  CTGGGCTTCAGTATCGCAGGTGGCATCGACAATCCCCATGTCCCTGATGACCCTGGCATCTTTATTACCA
  AGATTATCCCTGGTGGAGCAGCTGCCATGGATGGGAGGCTGGGGGTGAATGACTGTGTGCTGCGGGTGAA
  TGAGGTGGACGTGTCGGAGGTGGTACACAGCCGGGCGGTGGAGGCGCTGAAGGAGGCAGGCCCTGTGGTG
  CGATTGGTGGTGCGGAGGCGACAGCCTCCACCCGAGACCATCATGGAGGTCAACCTGCTCAAAGGGCCCA
  AAGGCCTGGGTTTCAGCATTGCTGGGGGTATTGGCAACCAGCACATCCCAGGAGACAACAGCATCTACAT
  CACCAAGATCATTGAGGGGGGTGCTGCTCAGAAGGATGGACGCCTACAGATTGGGGACCGGCTGCTGGCG
  GTGAACAACACCAATCTGCAGGATGTGAGGCACGAGGAAGCTGTGGCCTCACTGAAGAACACATCTGATA
  TGGTGTATTTGAAGGTGGCCAAGCCAGGCAGCCTCCACCTCAACGACATGTACGCTCCCCCTGACTACGC
  CAGCACTTTTACTGCCTTGGCTGACAACCACATAAGCCATAATTCCAGCCTGGGTTATCTCGGGGCTGTG
  GAGAGCAAGGTCAGCTACCCTGCTCCTCCTCAGGTTCCCCCCACCCGCTACTCTCCTATTCCCAGGCACA
  TGCTGGCTGAGGAGGACTTCACCAGAGAGCCTCGCAAGATCATCCTGCACAAAGGCTCCACAGGCCTGGG
  CTTCAACATCGTAGGAGGAGAGGATGGAGAAGGCATTTTTGTCTCCTTCATCCTGGCAGGAGGCCCAGCT
  GACCTGAGTGGGGAGCTGCGCAGGGGAGACCGGATCTTATCGGTGAATGGAGTGAATCTGAGGAATGCAA
  CTCATGAGCAGGCTGCAGCTGCTCTGAAACGGGCCGGCCAGTCAGTCACCATTGTGGCCCAGTACAGACC
  TGAAGAATACAGTCGCTTTGAATCGAAGATACATGACTTACGAGAACAAATGATGAACAGCAGCATGAGC
  TCTGGGTCTGGGTCCCTCCGAACAAGTGAAAAGAGGTCCTTGTATGTCAGGGCCCTGTTTGATTATGATC
  GGACTCGGGACAGCTGCCTGCCAAGCCAGGGGCTCAGCTTCTCTTATGGTGACATTCTGCATGTCATTAA
  TGCCTCTGATGATGAGTGGTGGCAGGCAAGGCTGGTGACCCCACACGGAGAAAGTGAGCAGATCGGTGTG
  ATCCCCAGTAAGAAGAGGGTGGAAAAGAAAGAAAGAGCTCGATTGAAAACTGTGAAGTTCCATGCCAGGA
  CGGGGATGATTGAGTCTAACAGGGACTTCCCGGGGTTAAGTGACGATTATTATGGAGCAAAGAACCTGAA
  AGGACAAGAGGATGCTATTTTGTCATATGAGCCAGTGACACGGCAAGAAATTCACTATGCAAGGCCTGTG
  ATCATCCTGGGCCCAATGAAGGACCGAGTCAATGATGACCTGATCTCCGAATTTCCACATAAATTTGGAT
  CCTGTGTGCCACATACTACCCGGCCTCGACGTGATAATGAGGTGGATGGACAAGACTACCACTTTGTGGT
  GTCCCGAGAACAAATGGAGAAAGATATTCAGGACAACAAGTTCATCGAGGCGGGCCAATTTAATGATAAC
  CTCTATGGGACCAGCATCCAGTCAGTGCGGGCAGTTGCAGAGAGGGGCAAGCACTGCATCTTAGATGTTT
  CCGGCAATGCTATCAAGAGACTGCAGCAAGCACAACTTTACCCCATTGCCATTTTCATCAAGCCCAAGTC
  CATTGAAGCCCTTATGGAAATGAACCGAAGGCAGACATATGAACAAGCAAATAAGATCTATGACAAAGCC
  ATGAAACTGGAGCAGGAATTTGGAGAGTACTTTACAGCCATTGTACAGGGTGACTCACTGGAAGAGATTT
  ATAACAAAATCAAACAAATCATTGAGGACCAGTCTGGGCACTACATTTGGGTCCCATCCCCTGAAAAACT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210860 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPGGGNGASAGYGGYSSQTLPSQAGATPTPR
  TKAKLIPTGRDVGPVPPKPVPGKSTPKLNGSGPSWWPECTCTNRDWYEQVNGSDGMFKYEEIVLERGNSG
  LGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVV
  RLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLA
  VNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSLHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAV
  ESKVSYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPA
  DLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMS
  SGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGV
  IPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGQEDAILSYEPVTRQEIHYARPV
  IILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDN
  LYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIYDKA
  MKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021120
• ORF Size: 2451 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021120.4
• RefSeq Size: 6032 bp
• RefSeq ORF: 2454 bp
• Locus ID: 1741
• UniProt ID: Q92796
• Cytogenetics: Xq13.1
• Domains: SH3, PDZ, Guanylate_kin, GuKc
• MW: 90.3 kDa
• Gene Summary: This gene encodes a member of the membrane-associated guanylate kinase protein family. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked cognitive disability. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]