SLC28A3 (NM_022127) Human Tagged ORF Clone

CAT#: RC210847

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SLC28A3 (Myc-DDK-tagged)-Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2



  "NM_022127" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SLC28A3
Synonyms CNT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210847 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGAGGAGTACAGCAGCCCCCAGAGCTGAGGGCTACAGCAACGTGGGCTTCCAGAATGAAGAAA
ACTTTCTTGAGAACGAGAACACATCAGGAAACAACTCAATAAGAAGCAGAGCTGTGCAAAGCAGGGAGCA
CACAAACACCAAACAGGATGAAGAACAGGTCACAGTTGAGCAGGATTCTCCAAGAAACAGAGAACACATG
GAGGATGATGATGAGGAGATGCAACAAAAAGGGTGTTTGGAAAGGAGGTATGACACGGTATGTGGTTTCT
GTAGGAAACACAAAACAACTCTTCGGCACATCATCTGGGGCATTTTATTAGCAGGTTATCTGGTTATGGT
GATTTCGGCCTGTGTGCTGAACTTTCACAGAGCCCTTCCTCTTTTTGTGATCACCGTGGCTGCCATCTTC
TTTGTTGTCTGGGATCACCTGATGGCCAAATACGAACATCGAATTGATGAGATGCTGTCTCCTGGCAGAA
GGCTTCTAAACAGCCATTGGTTCTGGCTGAAGTGGGTGATCTGGAGCTCCCTGGTCCTAGCAGTTATTTT
CTGGTTGGCCTTTGACACTGCCAAATTGGGTCAACAGCAGCTGGTGTCCTTCGGTGGGCTCATAATGTAC
ATTGTCCTGTTATTTCTATTCTCCAAGTACCCAACCAGAGTTTACTGGAGACCTGTCTTATGGGGAATCG
GGCTACAGTTTCTTCTTGGGCTCTTGATTCTAAGGACTGACCCTGGATTTATAGCTTTTGATTGGTTGGG
CAGACAAGTTCAGACTTTTCTGGAGTACACAGATGCTGGTGCTTCATTTGTCTTTGGTGAGAAATACAAA
GACCACTTCTTTGCATTTAAGGTCCTGCCGATCGTGGTTTTCTTCAGCACTGTGATGTCCATGCTGTACT
ACCTGGGACTGATGCAGTGGATTATTAGAAAGGTTGGATGGATCATGCTAGTTACTACGGGATCATCTCC
TATTGAATCTGTAGTTGCTTCTGGCAATATATTTGTTGGACAAACGGAGTCTCCACTGCTGGTCCGACCA
TATTTACCTTACATCACCAAGTCTGAACTCCACGCCATCATGACCGCCGGGTTCTCTACCATTGCTGGAA
GCGTGCTAGGTGCATACATTTCTTTTGGGGTTCCATCCTCCCACTTGTTAACAGCGTCAGTTATGTCAGC
ACCTGCGTCATTGGCTGCTGCTAAACTCTTTTGGCCTGAGACAGAAAAACCTAAAATAACCCTCAAGAAT
GCCATGAAAATGGAAAGTGGTGATTCAGGGAATCTTCTAGAAGCTGCAACACAGGGAGCATCCTCCTCCA
TCTCCCTGGTGGCCAACATCGCTGTGAATCTGATTGCCTTCCTGGCCCTGCTGTCTTTTATGAATTCAGC
CCTGTCCTGGTTTGGAAACATGTTTGACTACCCACAGCTGAGTTTTGAGCTAATCTGCTCCTACATCTTC
ATGCCCTTTTCCTTCATGATGGGAGTGGAATGGCAGGACAGCTTTATGGTTGCCAGACTCATAGGTTATA
AGACCTTCTTCAATGAATTTGTGGCTTATGAGCACCTCTCAAAATGGATCCACTTGAGGAAAGAAGGTGG
ACCCAAATTTGTAAACGGTGTGCAGCAATATATATCAATTCGTTCTGAGATAATCGCCACTTACGCTCTC
TGTGGTTTTGCCAATATCGGGTCCCTAGGAATCGTGATCGGCGGACTCACATCCATGGCTCCTTCCAGAA
AGCGTGATATCGCCTCGGGGGCAGTGAGAGCTCTGATTGCGGGGACCGTGGCCTGCTTCATGACAGCCTG
CATCGCAGGCATACTCTCCAGCACTCCTGTGGACATCAACTGCCATCACGTTTTAGAGAATGCCTTCAAC
TCCACTTTCCCTGGAAACACAACCAAGGTGATAGCTTGTTGCCAAAGTCTGTTGAGCAGCACTGTTGCCA
AGGGTCCTGGTGAAGTCATCCCAGGAGGAAACCACAGTCTGTATTCTTTGAAGGGCTGCTGCACATTGTT
GAATCCATCGACCTTTAACTGCAATGGGATCTCTAATACATTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210847 protein sequence
Red=Cloning site Green=Tags(s)

MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSRAVQSREHTNTKQDEEQVTVEQDSPRNREHM
EDDDEEMQQKGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVISACVLNFHRALPLFVITVAAIF
FVVWDHLMAKYEHRIDEMLSPGRRLLNSHWFWLKWVIWSSLVLAVIFWLAFDTAKLGQQQLVSFGGLIMY
IVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFGEKYK
DHFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRP
YLPYITKSELHAIMTAGFSTIAGSVLGAYISFGVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKN
AMKMESGDSGNLLEAATQGASSSISLVANIAVNLIAFLALLSFMNSALSWFGNMFDYPQLSFELICSYIF
MPFSFMMGVEWQDSFMVARLIGYKTFFNEFVAYEHLSKWIHLRKEGGPKFVNGVQQYISIRSEIIATYAL
CGFANIGSLGIVIGGLTSMAPSRKRDIASGAVRALIAGTVACFMTACIAGILSSTPVDINCHHVLENAFN
STFPGNTTKVIACCQSLLSSTVAKGPGEVIPGGNHSLYSLKGCCTLLNPSTFNCNGISNTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022127
ORF Size 2073 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022127.3
RefSeq Size 4573 bp
RefSeq ORF 2076 bp
Locus ID 64078
UniProt ID Q9HAS3
Cytogenetics 9q21.32-q21.33
Domains Nucleoside_tra2
Protein Families Transmembrane
MW 76.9 kDa
Gene Summary Nucleoside transporters, such as SLC28A3, regulate multiple cellular processes, including neurotransmission, vascular tone, adenosine concentration in the vicinity of cell surface receptors, and transport and metabolism of nucleoside drugs. SLC28A3 shows broad specificity for pyrimidine and purine nucleosides (Ritzel et al., 2001 [PubMed 11032837]).[supplied by OMIM, Mar 2008]

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