SLC28A3 (NM_022127) Human Tagged ORF Clone

CAT#: RC210847

SLC28A3 (Myc-DDK-tagged)-Human solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (SLC28A3), transcript variant 2

Product Images

Circular map for RC210847

• 

  Western blot validation of overexpression lysate (Cat# LY402908) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210847 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC28A3
• Synonyms: CNT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210847 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGAGGAGTACAGCAGCCCCCAGAGCTGAGGGCTACAGCAACGTGGGCTTCCAGAATGAAGAAA
  ACTTTCTTGAGAACGAGAACACATCAGGAAACAACTCAATAAGAAGCAGAGCTGTGCAAAGCAGGGAGCA
  CACAAACACCAAACAGGATGAAGAACAGGTCACAGTTGAGCAGGATTCTCCAAGAAACAGAGAACACATG
  GAGGATGATGATGAGGAGATGCAACAAAAAGGGTGTTTGGAAAGGAGGTATGACACGGTATGTGGTTTCT
  GTAGGAAACACAAAACAACTCTTCGGCACATCATCTGGGGCATTTTATTAGCAGGTTATCTGGTTATGGT
  GATTTCGGCCTGTGTGCTGAACTTTCACAGAGCCCTTCCTCTTTTTGTGATCACCGTGGCTGCCATCTTC
  TTTGTTGTCTGGGATCACCTGATGGCCAAATACGAACATCGAATTGATGAGATGCTGTCTCCTGGCAGAA
  GGCTTCTAAACAGCCATTGGTTCTGGCTGAAGTGGGTGATCTGGAGCTCCCTGGTCCTAGCAGTTATTTT
  CTGGTTGGCCTTTGACACTGCCAAATTGGGTCAACAGCAGCTGGTGTCCTTCGGTGGGCTCATAATGTAC
  ATTGTCCTGTTATTTCTATTCTCCAAGTACCCAACCAGAGTTTACTGGAGACCTGTCTTATGGGGAATCG
  GGCTACAGTTTCTTCTTGGGCTCTTGATTCTAAGGACTGACCCTGGATTTATAGCTTTTGATTGGTTGGG
  CAGACAAGTTCAGACTTTTCTGGAGTACACAGATGCTGGTGCTTCATTTGTCTTTGGTGAGAAATACAAA
  GACCACTTCTTTGCATTTAAGGTCCTGCCGATCGTGGTTTTCTTCAGCACTGTGATGTCCATGCTGTACT
  ACCTGGGACTGATGCAGTGGATTATTAGAAAGGTTGGATGGATCATGCTAGTTACTACGGGATCATCTCC
  TATTGAATCTGTAGTTGCTTCTGGCAATATATTTGTTGGACAAACGGAGTCTCCACTGCTGGTCCGACCA
  TATTTACCTTACATCACCAAGTCTGAACTCCACGCCATCATGACCGCCGGGTTCTCTACCATTGCTGGAA
  GCGTGCTAGGTGCATACATTTCTTTTGGGGTTCCATCCTCCCACTTGTTAACAGCGTCAGTTATGTCAGC
  ACCTGCGTCATTGGCTGCTGCTAAACTCTTTTGGCCTGAGACAGAAAAACCTAAAATAACCCTCAAGAAT
  GCCATGAAAATGGAAAGTGGTGATTCAGGGAATCTTCTAGAAGCTGCAACACAGGGAGCATCCTCCTCCA
  TCTCCCTGGTGGCCAACATCGCTGTGAATCTGATTGCCTTCCTGGCCCTGCTGTCTTTTATGAATTCAGC
  CCTGTCCTGGTTTGGAAACATGTTTGACTACCCACAGCTGAGTTTTGAGCTAATCTGCTCCTACATCTTC
  ATGCCCTTTTCCTTCATGATGGGAGTGGAATGGCAGGACAGCTTTATGGTTGCCAGACTCATAGGTTATA
  AGACCTTCTTCAATGAATTTGTGGCTTATGAGCACCTCTCAAAATGGATCCACTTGAGGAAAGAAGGTGG
  ACCCAAATTTGTAAACGGTGTGCAGCAATATATATCAATTCGTTCTGAGATAATCGCCACTTACGCTCTC
  TGTGGTTTTGCCAATATCGGGTCCCTAGGAATCGTGATCGGCGGACTCACATCCATGGCTCCTTCCAGAA
  AGCGTGATATCGCCTCGGGGGCAGTGAGAGCTCTGATTGCGGGGACCGTGGCCTGCTTCATGACAGCCTG
  CATCGCAGGCATACTCTCCAGCACTCCTGTGGACATCAACTGCCATCACGTTTTAGAGAATGCCTTCAAC
  TCCACTTTCCCTGGAAACACAACCAAGGTGATAGCTTGTTGCCAAAGTCTGTTGAGCAGCACTGTTGCCA
  AGGGTCCTGGTGAAGTCATCCCAGGAGGAAACCACAGTCTGTATTCTTTGAAGGGCTGCTGCACATTGTT
  GAATCCATCGACCTTTAACTGCAATGGGATCTCTAATACATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210847 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSRAVQSREHTNTKQDEEQVTVEQDSPRNREHM
  EDDDEEMQQKGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVISACVLNFHRALPLFVITVAAIF
  FVVWDHLMAKYEHRIDEMLSPGRRLLNSHWFWLKWVIWSSLVLAVIFWLAFDTAKLGQQQLVSFGGLIMY
  IVLLFLFSKYPTRVYWRPVLWGIGLQFLLGLLILRTDPGFIAFDWLGRQVQTFLEYTDAGASFVFGEKYK
  DHFFAFKVLPIVVFFSTVMSMLYYLGLMQWIIRKVGWIMLVTTGSSPIESVVASGNIFVGQTESPLLVRP
  YLPYITKSELHAIMTAGFSTIAGSVLGAYISFGVPSSHLLTASVMSAPASLAAAKLFWPETEKPKITLKN
  AMKMESGDSGNLLEAATQGASSSISLVANIAVNLIAFLALLSFMNSALSWFGNMFDYPQLSFELICSYIF
  MPFSFMMGVEWQDSFMVARLIGYKTFFNEFVAYEHLSKWIHLRKEGGPKFVNGVQQYISIRSEIIATYAL
  CGFANIGSLGIVIGGLTSMAPSRKRDIASGAVRALIAGTVACFMTACIAGILSSTPVDINCHHVLENAFN
  STFPGNTTKVIACCQSLLSSTVAKGPGEVIPGGNHSLYSLKGCCTLLNPSTFNCNGISNTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022127
• ORF Size: 2073 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022127.3
• RefSeq Size: 4573 bp
• RefSeq ORF: 2076 bp
• Locus ID: 64078
• UniProt ID: Q9HAS3
• Cytogenetics: 9q21.32-q21.33
• Domains: Nucleoside_tra2
• Protein Families: Transmembrane
• MW: 76.9 kDa
• Gene Summary: Nucleoside transporters, such as SLC28A3, regulate multiple cellular processes, including neurotransmission, vascular tone, adenosine concentration in the vicinity of cell surface receptors, and transport and metabolism of nucleoside drugs. SLC28A3 shows broad specificity for pyrimidine and purine nucleosides (Ritzel et al., 2001 [PubMed 11032837]).[supplied by OMIM, Mar 2008]