Serum Amyloid A (SAA1) (NM_000331) Human Tagged ORF Clone
SAA1 (Myc-DDK-tagged)-Human serum amyloid A1 (SAA1), transcript variant 1
|Type||Human Tagged ORF Clone|
|Synonyms||PIG4; SAA; SAA2; TP53I4|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC210664 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210664 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||366 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||678 bp|
|RefSeq ORF||369 bp|
|Gene Summary||This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Finally, antimicrobial activity against S. aureus and E. coli resides in the N-terminal portion of the mature protein. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11. [provided by RefSeq, Jul 2020]|
|cDNA Clone Resources|
|RC210664L1||Lenti ORF clone of Human serum amyloid A1 (SAA1), transcript variant 1, Myc-DDK-tagged||
|RC210664L2||Lenti ORF clone of Human serum amyloid A1 (SAA1), transcript variant 1, mGFP tagged||
|RC210664L3||Lenti ORF clone of Human serum amyloid A1 (SAA1), transcript variant 1, Myc-DDK-tagged||
|RC210664L4||Lenti ORF clone of Human serum amyloid A1 (SAA1), transcript variant 1, mGFP tagged||
|RG210664||SAA1 (GFP-tagged) - Human serum amyloid A1 (SAA1), transcript variant 1||
|SC300047||SAA1 (untagged)-Human serum amyloid A1 (SAA1), transcript variant 1||