PD1 (PDCD1) (NM_005018) Human Tagged ORF Clone
PD-1 / PDCD1 (Myc-DDK-tagged)-Human programmed cell death 1 (PD-1 / PDCD1)
|Type||Human Tagged ORF Clone|
|Synonyms||CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC210364 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210364 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||864 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2115 bp|
|RefSeq ORF||867 bp|
|Domains||ig, IGv, IG|
|Protein Families||Druggable Genome, Transmembrane|
|Protein Pathways||Cell adhesion molecules (CAMs), T cell receptor signaling pathway|
|Gene Summary||Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020]|
|cDNA Clone Resources|
|RC210364L1||Lenti ORF clone of Human programmed cell death 1 (PDCD1), Myc-DDK-tagged||
|RC210364L2||Lenti ORF clone of Human programmed cell death 1 (PDCD1), mGFP tagged||
|RC210364L3||Lenti ORF clone of Human programmed cell death 1 (PDCD1), Myc-DDK-tagged||
|RC210364L4||Lenti ORF clone of Human programmed cell death 1 (PDCD1), mGFP tagged||
|RG210364||PD-1 / PDCD1 (GFP-tagged) - Human programmed cell death 1 (PD-1 / PDCD1)||
|SC117011||PD-1 / PDCD1 (untagged)-Human programmed cell death 1 (PD-1 / PDCD1)||