Serotonin transporter (SLC6A4) (NM_001045) Human Tagged ORF Clone

CAT#: RC210187

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SLC6A4 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4)



  "NM_001045" in other vectors (6)

Reconstitution Protocol

USD 879.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SLC6A4
Synonyms 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; SERT; SERT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210187 representing NM_001045
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACGACGCCCTTGAATTCTCAGAAGCAGCTATCAGCGTGTGAAGATGGAGAAGATTGTCAGGAAA
ACGGAGTTCTACAGAAGGTTGTTCCCACCCCAGGGGACAAAGTGGAGTCCGGGCAAATATCCAATGGGTA
CTCAGCAGTTCCAAGTCCTGGTGCGGGAGATGACACACGGCACTCTATCCCAGCGACCACCACCACCCTA
GTGGCTGAGCTTCATCAAGGGGAACGGGAGACCTGGGGCAAGAAGGTGGATTTCCTTCTCTCAGTGATTG
GCTATGCTGTGGACCTGGGCAATGTCTGGCGCTTCCCCTACATATGTTACCAGAATGGAGGGGGGGCATT
CCTCCTCCCCTACACCATCATGGCCATTTTTGGGGGAATCCCGCTCTTTTACATGGAGCTCGCACTGGGA
CAGTACCACCGAAATGGATGCATTTCAATATGGAGGAAAATCTGCCCGATTTTCAAAGGGATTGGTTATG
CCATCTGCATCATTGCCTTTTACATTGCTTCCTACTACAACACCATCATGGCCTGGGCGCTATACTACCT
CATCTCCTCCTTCACGGACCAGCTGCCCTGGACCAGCTGCAAGAACTCCTGGAACACTGGCAACTGCACC
AATTACTTCTCCGAGGACAACATCACCTGGACCCTCCATTCCACGTCCCCTGCTGAAGAATTTTACACGC
GCCACGTCCTGCAGATCCACCGGTCTAAGGGGCTCCAGGACCTGGGGGGCATCAGCTGGCAGCTGGCCCT
CTGCATCATGCTGATCTTCACTGTTATCTACTTCAGCATCTGGAAAGGCGTCAAGACCTCTGGCAAGGTG
GTGTGGGTGACAGCCACCTTCCCTTATATCATCCTTTCTGTCCTGCTGGTGAGGGGTGCCACCCTCCCTG
GAGCCTGGAGGGGTGTTCTCTTCTACTTGAAACCCAATTGGCAGAAACTCCTGGAGACAGGGGTGTGGAT
AGATGCAGCCGCTCAGATCTTCTTCTCTCTTGGTCCGGGCTTTGGGGTCCTGCTGGCTTTTGCTAGCTAC
AACAAGTTCAACAACAACTGCTACCAAGATGCCCTGGTGACCAGCGTGGTGAACTGCATGACGAGCTTCG
TTTCGGGATTTGTCATCTTCACAGTGCTCGGTTACATGGCTGAGATGAGGAATGAAGATGTGTCTGAGGT
GGCCAAAGACGCAGGTCCCAGCCTCCTCTTCATCACGTATGCAGAAGCGATAGCCAACATGCCAGCGTCC
ACTTTCTTTGCCATCATCTTCTTTCTGATGTTAATCACGCTGGGCTTGGACAGCACGTTTGCAGGCTTGG
AGGGGGTGATCACGGCTGTGCTGGATGAGTTCCCACACGTCTGGGCCAAGCGCCGGGAGCGGTTCGTGCT
CGCCGTGGTCATCACCTGCTTCTTTGGATCCCTGGTCACCCTGACTTTTGGAGGGGCCTACGTGGTGAAG
CTGCTGGAGGAGTATGCCACGGGGCCCGCAGTGCTCACTGTCGCGCTGATCGAAGCAGTCGCTGTGTCTT
GGTTCTATGGCATCACTCAGTTCTGCAGGGACGTGAAGGAAATGCTCGGCTTCAGCCCGGGGTGGTTCTG
GAGGATCTGCTGGGTGGCCATCAGCCCTCTGTTTCTCCTGTTCATCATTTGCAGTTTTCTGATGAGCCCG
CCACAACTACGACTTTTCCAATATAATTATCCTTACTGGAGTATCATCTTGGGTTACTGCATAGGAACCT
CATCTTTCATTTGCATCCCCACATATATAGCTTATCGGTTGATCATCACTCCAGGGACATTTAAAGAGCG
TATTATTAAAAGTATTACCCCAGAAACACCAACAGAAATTCCTTGTGGGGACATCCGCTTGAATGCTGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210187 representing NM_001045
Red=Cloning site Green=Tags(s)

METTPLNSQKQLSACEDGEDCQENGVLQKVVPTPGDKVESGQISNGYSAVPSPGAGDDTRHSIPATTTTL
VAELHQGERETWGKKVDFLLSVIGYAVDLGNVWRFPYICYQNGGGAFLLPYTIMAIFGGIPLFYMELALG
QYHRNGCISIWRKICPIFKGIGYAICIIAFYIASYYNTIMAWALYYLISSFTDQLPWTSCKNSWNTGNCT
NYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGGISWQLALCIMLIFTVIYFSIWKGVKTSGKV
VWVTATFPYIILSVLLVRGATLPGAWRGVLFYLKPNWQKLLETGVWIDAAAQIFFSLGPGFGVLLAFASY
NKFNNNCYQDALVTSVVNCMTSFVSGFVIFTVLGYMAEMRNEDVSEVAKDAGPSLLFITYAEAIANMPAS
TFFAIIFFLMLITLGLDSTFAGLEGVITAVLDEFPHVWAKRRERFVLAVVITCFFGSLVTLTFGGAYVVK
LLEEYATGPAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIICSFLMSP
PQLRLFQYNYPYWSIILGYCIGTSSFICIPTYIAYRLIITPGTFKERIIKSITPETPTEIPCGDIRLNAV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001045
ORF Size 1890 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001045.6
RefSeq Size 2756 bp
RefSeq ORF 1893 bp
Locus ID 6532
UniProt ID P31645
Cytogenetics 17q11.2
Domains SNF, 5HT_transporter
Protein Families Druggable Genome, Transmembrane
MW 70.1 kDa
Gene Summary This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake. There have been conflicting results in the literature about the possible effect, if any, that this polymorphism may play in behavior and depression. [provided by RefSeq, May 2019]

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