EIF4EBP3 (NM_003732) Human Tagged ORF Clone

CAT#: RC210053

EIF4EBP3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E binding protein 3 (EIF4EBP3)

Product Images

Circular map for RC210053

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF4EBP3 (Cat# RC210053, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4EBP3 (Cat# TA807773)(1:2000). Positive lysates LY418472 (100ug) and LC418472 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418472) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210053 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EIF4EBP3 protein (Cat# TP310053). The protein was produced from HEK293T cells transfected with EIF4EBP3 cDNA clone (Cat# RC210053) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF4EBP3
• Synonyms: 4E-BP3; 4EBP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210053 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACGTCCACTAGCTGCCCGATTCCCGGGGGCCGGGACCAGCTGCCCGACTGCTACAGCACCACGC
  CGGGGGGCACGCTATACGCCACTACCCCCGGAGGCACCAGGATCATCTACGACCGAAAGTTCCTGCTGGA
  GTGCAAGAACTCACCCATTGCCCGGACACCCCCCTGCTGCCTCCCTCAGATTCCCGGGGTCACAACTCCT
  CCAACAGCCCCTCTCTCCAAGCTGGAGGAGCTGAAGGAGCAGGAGACAGAGGAAGAGATACCCGATGACG
  CACAATTTGAAATGGACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210053 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTSTSCPIPGGRDQLPDCYSTTPGGTLYATTPGGTRIIYDRKFLLECKNSPIARTPPCCLPQIPGVTTP
  PTAPLSKLEELKEQETEEEIPDDAQFEMDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003732
• ORF Size: 300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003732.3
• RefSeq Size: 728 bp
• RefSeq ORF: 303 bp
• Locus ID: 8637
• UniProt ID: O60516
• Cytogenetics: 5q31.3
• MW: 10.9 kDa
• Gene Summary: This gene encodes a member of the EIF4EBP family, which consists of proteins that bind to eukaryotic translation initiation factor 4E and regulate its assembly into EIF4F, the multi-subunit translation initiation factor that recognizes the mRNA cap structure. Read-through transcription from the neighboring upstream gene (MASK or ANKHD1) generates a transcript (MASK-BP3) that encodes a protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Oct 2010]