EIF4EBP3 (NM_003732) Human Untagged Clone

CAT#: SC117797

EIF4EBP3 (untagged)-Human eukaryotic translation initiation factor 4E binding protein 3 (EIF4EBP3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4EBP3
• Synonyms: 4E-BP3; 4EBP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117797 sequence for NM_003732 edited (data generated by NextGen Sequencing)
  ATGTCAACGTCCACTAGCTGCCCGATTCCCGGGGGCCGGGACCAGCTGCCCGACTGCTAC
  AGCACCACGCCGGGGGGCACGCTATACGCCACTACCCCCGGAGGCACCAGGATCATCTAC
  GACCGAAAGTTCCTGCTGGAGTGCAAGAACTCACCCATTGCCCGGACACCCCCCTGCTGC
  CTCCCTCAGATTCCCGGGGTCACAACTCCTCCAACAGCCCCTCTCTCCAAGCTGGAGGAG
  CTGAAGGAGCAGGAGACAGAGGAAGAGATACCCGATGACGCACAATTTGAAATGGACATC
  TAA
  
  Clone variation with respect to NM_003732.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003732 unedited
  NTGTTCAAAATTTGTNATACGAACTCACTATAGGGCGGCCGCGAATCGGCACCAGCGGCT
  GAGTCCGCCTCAGACTCAGAGCTGCGCTCCTCGACCTCAACGCCAGGCGGTTACTTTGCT
  GCTCCTCCCGCTCGCTATGTCAACGTCCACTAGCTGCCCGATTCCCGGGGGCCGGGACCA
  GCTGCCCGACTGCTACAGCACCACGCCGGGGGGCACGCTATACGCCACTACCCCCGGAGG
  CACCAGGATCATCTACGACCGAAAGTTCCTGCTGGAGTGCAAGAACTCACCCATTGCCCG
  GACACCCCCCTGCTGCCTCCCTCAGATTCCCGGGGTCACAACTCCTCCAACAGCCCCTCT
  CTCCAAGCTGGAGGAGCTGAAGGAGCAGGAGACAGAGGAAGAGATACCCGATGACGCACA
  ATTTGAAATGGACATCTAATCCAGTGCAGATGACCTGGCATGTGGAGTTACAGAGGGATC
  CCTCATGCCACTGCTGCCACCACCTCTTCCTGGGGCATCCAAAGGCCAGCTGGCCTCATC
  TAATCTGGAAGGGAGTGACTTGCTAGTTCCAGGCCTCCTTTAGTTCTGAGGCAGCTAGAC
  CAGGGATAGGAGTGGGGCAACTTGCCAAGCCCTTAACTCTACTTCCTCTTCAGTCTGTGG
  TACTCCTCCCTAACCCTAAACCCTCTATGCTCAGGGGCTGGAACTGNCGAATGGAGTAAG
  TCACCTTCTGACTGCTTAGTAAACACTCAAACCCCAAAAACACCACACAAAAAAACTCGA
  CTCTAGATGGCGCCCGGGTCATACCTGTTTCCTGAAAGATCCCGTGTGCAATCCTGGGAC
  CCCTCCCCAGGCCTTCCTGGCCTTGAAGTGCACCTCCATGCCACCACCCTGTCCTAAAAA
  ATAAGTGCACATTCTGCCGACTAGGGCCCTTATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003732 unedited
  ACTATGGACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTGAATGGTTACTAAGCAGTCAGAAGGTGACTTACTCCATTCCCCAGTTCCAGCCCCTGA
  GCATAAAGGGTTTAGGGTTAGGAGGAGTACCACAGACTGAAGAGGAAGTAGAGTTAAGGG
  CTTGGCAAGTTGCCCACTCCTATCCCTGGTCTAGCTGCCTCAAAACTAAAGGAGGCCTGG
  AACTAACAAGTCACTCCCTTCCAAATTAAATGAGGCCAGCTGGCCTTTGGATGCCCCAGG
  AAAAGGTGGTGGCAGCAGTGGCATGAGGGATCCCTCTGTAACTCCACATGCCAGGTCATC
  TGCACTGGATTAAATGTCCATTTCAAATTGTGCGTCATCGGGTATCTCTTCCTCTGTCTC
  CTGCTCCTTCAGCTCCTCCAACTTGGAAAGAGGGGCTGTTGGAGGAGTTGTGACCCCGGG
  AATCTGAGGGAGGCAGCAGGGGGGTGTCCGGGCAATGGGTGAGTTCTTGCACTCCAGCAG
  GAACTTTCGGTCGTAGATGATCCTGGTGCCTCCGGGGGTAGTGGCGTATAGCGTGCCCCC
  CGGCGTGGTGCTGTANCAGTCGGGCAGCTGGTCCCGGCCCCCGGGAATCGGGCAGCTAGT
  GGACGTTGACATATCGAGCGGGAGGAGCAGCAAAGTTACCGCCCTGCGGTTGAAGTCCAG
  GGACCGCACCTTCTGATCTGAAGGGGGACTCAACCGCTTTGGTGCCAAATCCCCGCCCCC
  CCTATAATGAAGCCGAATTACCAAATTTTGACCGGTTACTTAAAGAACTTTTGTTATTAA
  AACCTTCCACCGTACACCCCTACCGCCCATTTTTGTTAACGGGGGGGGGGTTATTAACAC
  TTTTGGAAAAGCCCCCCTGTTTCGTCGGCCCCAAACAACTCCCCTTGACGTTAATGGGGG
  TGAAACTTCGCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003732
• Insert Size: 720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003732.2, NP_003723.1
• RefSeq Size: 728 bp
• RefSeq ORF: 303 bp
• Locus ID: 8637
• UniProt ID: O60516
• Cytogenetics: 5q31.3
• Gene Summary: This gene encodes a member of the EIF4EBP family, which consists of proteins that bind to eukaryotic translation initiation factor 4E and regulate its assembly into EIF4F, the multi-subunit translation initiation factor that recognizes the mRNA cap structure. Read-through transcription from the neighboring upstream gene (MASK or ANKHD1) generates a transcript (MASK-BP3) that encodes a protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Oct 2010]