PIGH (NM_004569) Human Tagged ORF Clone
CAT#: RC210028
PIGH (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class H (PIGH)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_004569" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PIGH |
Synonyms | GPI-H |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210028 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGAGCGGAGCTTTTCGGATATCTGCGGCGGCCGCCTGGCGCTGCAGCGCCGCTACTACTCCC CGTCCTGCCGGGAATTCTGCCTCAGCTGCCCTCGGCTCTCGCTGCGTTCGCTCACCGCTGTCACCTGCAC GGTGTGGCTGGCGGCCTACGGACTCTTCACCCTCTGCGAGAACAGCATGATCCTCTCTGCTGCCATCTTC ATCACCCTCTTAGGTCTGCTTGGTTATCTCCATTTTGTGAAGATTGATCAGGAGACTCTGTTAATCATTG ATTCCCTTGGCATTCAGATGACTTCATCTTATGCTTCAGGCAAAGAAAGCACTACCTTCATAGAAATGGG CAAGGTCAAGGATATTGTCATCAATGAGGCCATTTACATGCAGAAGGTGATTTACTACCTCTGCATCTTA TTGAAAGATCCAGTGGAACCACATGGGATATCCCAAGTAGTACCCGTCTTCCAGAGTGCCAAGCCCCGGC TGGACTGCTTGATTGAAGTATACAGGAGCTGCCAGGAGATCCTGGCACACCAGAAAGCCACATCAACAAG CCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210028 protein sequence
Red=Cloning site Green=Tags(s) MEDERSFSDICGGRLALQRRYYSPSCREFCLSCPRLSLRSLTAVTCTVWLAAYGLFTLCENSMILSAAIF ITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVKDIVINEAIYMQKVIYYLCIL LKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRSCQEILAHQKATSTSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004569 |
ORF Size | 564 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004569.5 |
RefSeq Size | 1439 bp |
RefSeq ORF | 567 bp |
Locus ID | 5283 |
UniProt ID | Q14442 |
Cytogenetics | 14q24.1 |
Protein Families | Transmembrane |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
MW | 21.1 kDa |
Gene Summary | This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. The protein encoded by this gene is a subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210028L3 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class H (PIGH), Myc-DDK-tagged |
USD 600.00 |
|
RC210028L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class H (PIGH), mGFP tagged |
USD 600.00 |
|
RG210028 | PIGH (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class H (PIGH) |
USD 500.00 |
|
SC117284 | PIGH (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class H (PIGH) |
USD 300.00 |
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