USP36 (NM_025090) Human Tagged ORF Clone

CAT#: RC209781

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USP36 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 36 (USP36)



Reconstitution Protocol

USD 978.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol USP36
Synonyms DUB1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209781 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAATAGTGGATAAGTTGAAGGAGGCCCTGAAACCCGGCCGCAAGGACTCGGCTGATGATGGAGAAC
TGGGGAAGCTTCTTGCCTCCTCTGCCAAGAAGGTCCTTTTACAGAAAATCGAGTTCGAGCCAGCCAGCAA
GAGCTTCTCCTACCAGCTGGAGGCCTTAAAGAGCAAATATGTGTTGCTCAACCCCAAAACAGAGGGAGCT
AGTCGCCACAAGAGTGGAGATGACCCACCGGCCGGGAGACAGGGCAGTGAGCACACGTATGAGAGCTGTG
GTGACGGAGTCCCAGCCCCGCAGAAAGTGCTTTTCCCCACGGAGCGACTGTCTCTGAGGTGGGAGCGGGT
CTTCCGCGTGGGCGCAGGACTCCACAACCTTGGCAACACCTGCTTTCTCAATGCCACCATCCAGTGCTTG
ACCTACACACCACCTCTAGCCAACTACCTGCTCTCCAAGGAGCATGCTCGCAGCTGCCACCAGGGAAGCT
TCTGCATGCTGTGTGTCATGCAGAACCACATTGTCCAGGCCTTCGCCAACAGCGGCAACGCCATCAAGCC
CGTCTCCTTCATCCGAGACCTGAAAAAGATCGCCCGACACTTCCGCTTTGGGAACCAGGAGGACGCGCAT
GAGTTCCTGCGGTACACCATCGACGCCATGCAGAAAGCCTGCCTGAATGGCTGTGCCAAGTTGGATCGTC
AAACGCAGGCTACTACCTTGGTCCATCAAATTTTTGGAGGGTATCTCAGATCACGCGTGAAGTGCTCCGT
GTGCAAGAGCGTCTCGGACACCTACGACCCCTACTTGGACGTCGCGCTGGAGATCCGGCAAGCTGCGAAT
ATTGTGCGTGCTCTGGAACTTTTTGTGAAAGCAGATGTCCTGAGTGGAGAGAATGCCTACATGTGTGCTA
AATGCAAGAAGAAGGTTCCAGCCAGCAAGCGCTTCACCATCCACAGAACATCCAACGTCTTAACCCTTTC
CCTCAAGCGCTTTGCCAACTTCAGCGGGGGGAAGATCACCAAGGATGTAGGCTATCCGGAATTCCTCAAC
ATACGTCCGTATATGTCCCAGAATAATGGTGATCCTGTCATGTATGGACTCTATGCTGTCCTGGTGCACT
CGGGCTACAGCTGCCATGCCGGGCACTATTACTGCTACGTGAAGGCAAGCAATGGACAGTGGTACCAGAT
GAATGATTCCTTGGTCCATTCCAGCAACGTCAAGGTGGTTCTGAACCAGCAGGCCTACGTGCTGTTCTAT
CTGCGAATTCCAGGCTCTAAGAAAAGTCCCGAGGGCCTCATCTCCAGGACAGGCTCCTCCTCCCTTCCCG
GCCGCCCGAGTGTGATTCCAGATCACTCCAAGAAGAACATCGGCAATGGGATTATTTCCTCCCCACTGAC
TGGAAAGCGACAAGACTCTGGGACGATGAAGAAGCCGCACACCACTGAAGAGATTGGTGTGCCCATATCC
AGGAATGGCTCCACCCTGGGCCTGAAGTCCCAGAACGGCTGCATTCCTCCAAAGCTGCCCTCGGGGTCCC
CTTCCCCCAAACTCTCCCAGACACCCACACACATGCCAACCATCCTAGACGACCCTGGAAAGAAGGTGAA
GAAGCCAGCTCCTCCACAGCACTTTTCCCCCAGAACTGCTCAGGGGCTGCCTGGGACCAGCAACTCGAAT
AGCAGCAGATCTGGGAGCCAAAGGCAGGGCTCCTGGGACAGCAGGGATGTTGTCCTCTCTACCTCACCTA
AGCTCCTGGCTACAGCCACTGCCAACGGGCATGGGCTGAAGGGGAACGACGAGAGCGCTGGCCTCGACAG
GAGGGGCTCCAGCAGCTCCAGCCCAGAGCACTCGGCCAGCAGTGACTCCACCAAGGCCCCCCAGACCCCC
AGGAGTGGAGCGGCCCATCTCTGCGATTCTCAGGAAACGAACTGTTCCACCGCTGGCCACTCCAAAACGC
CGCCAAGTGGAGCAGATTCTAAGACGGTGAAGCTGAAGTCCCCTGTCCTGAGCAACACCACCACTGAGCC
TGCAAGCACCATGTCTCCTCCACCAGCCAAAAAACTGGCCCTTTCTGCCAAGAAGGCCAGCACCCTGTGG
AGGGCGACCGGCAATGACCTCCGTCCACCTCCCCCCTCACCATCCTCCGACCTCACCCACCCCATGAAAA
CCTCTCACCCCGTCGTTGCCTCCACTTGGCCCGTCCATAGAGCCAGGGCTGTGTCACCTGCTCCCCAATC
ATCCAGCCGCCTGCAACCCCCCTTCAGCCCCCACCCCACATTGCTGTCCAGTACCCCCAAGCCCCCAGGG
ACGTCAGAACCACGGAGCTGCTCCTCCATCTCGACGGCGCTGCCTCAGGTCAACGAGGACCTTGTGTCTC
TTCCACACCAGTTGCCAGAGGCCAGTGAGCCCCCCCAGAGCCCCTCTGAGAAGAGGAAAAAGACCTTTGT
GGGAGAGCCGCAGAGGCTGGGCTCAGAGACGCGCCTCCCACAGCACATCAGGGAGGCCACTGCGGCTCCC
CACGGGAAGAGGAAGAGGAAGAAGAAGAAGCGCCCGGAGGACACAGCTGCCAGCGCCCTGCAGGAGGGGC
AGACACAGAGACAGCCTGGGAGCCCCATGTACAGGAGGGAGGGCCAGGCACAGCTGCCCGCTGTCAGACG
GCAGGAAGATGGCACACAGCCACAGGTGAATGGCCAGCAGGTGGGATGTGTTACGGACGGCCACCACGCG
AGCAGCAGGAAGCGGAGGAGGAAAGGAGCAGAAGGTCTTGGTGAAGAAGGCGGCCTGCACCAGGACCCAC
TTCGGCACAGCTGCTCTCCCATGGGTGATGGTGATCCAGAGGCCATGGAAGAGTCTCCAAGGAAAAAGAA
AAAGAAAAAAAGAAAGCAGGAGACACAGCGGGCAGTAGAAGAGGATGGGCATCTCAAATGCCCAAGGAGT
GCCAAGCCCCAAGATGCTGTTGTCCCCGAGTCCAGCAGCTGCGCACCATCCGCGAATGGCTGGTGTCCTG
GGGACCGCATGGGGCTGAGCCAGGCCCCTCCTGTGTCTTGGAATGGAGAGCGGGAGTCTGATGTGGTCCA
GGAACTGCTCAAATACTCATCTGATAAAGCTTACGGGAGAAAAGTTCTGACCTGGGATGGCAAGATGTCG
GCGGTCAGTCAGGATGCTATTGAAGACAGCAGACAGGCCCGGACTGAGACCGTGGTTGATGACTGGGACG
AAGAGTTTGACCGAGGGAAGGAAAAGAAAATTAAAAAATTTAAGAGAGAGAAGAGGAGAAACTTCAACGC
CTTCCAGAAACTTCAGACTCGACGGAACTTCTGGTCTGTGACTCACCCAGCAAAGGCTGCCAGCCTCAGC
TATCGCCGC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC209781 protein sequence
Red=Cloning site Green=Tags(s)

MPIVDKLKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFSYQLEALKSKYVLLNPKTEGA
SRHKSGDDPPAGRQGSEHTYESCGDGVPAPQKVLFPTERLSLRWERVFRVGAGLHNLGNTCFLNATIQCL
TYTPPLANYLLSKEHARSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAH
EFLRYTIDAMQKACLNGCAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDVALEIRQAAN
IVRALELFVKADVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLN
IRPYMSQNNGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFY
LRIPGSKKSPEGLISRTGSSSLPGRPSVIPDHSKKNIGNGIISSPLTGKRQDSGTMKKPHTTEEIGVPIS
RNGSTLGLKSQNGCIPPKLPSGSPSPKLSQTPTHMPTILDDPGKKVKKPAPPQHFSPRTAQGLPGTSNSN
SSRSGSQRQGSWDSRDVVLSTSPKLLATATANGHGLKGNDESAGLDRRGSSSSSPEHSASSDSTKAPQTP
RSGAAHLCDSQETNCSTAGHSKTPPSGADSKTVKLKSPVLSNTTTEPASTMSPPPAKKLALSAKKASTLW
RATGNDLRPPPPSPSSDLTHPMKTSHPVVASTWPVHRARAVSPAPQSSSRLQPPFSPHPTLLSSTPKPPG
TSEPRSCSSISTALPQVNEDLVSLPHQLPEASEPPQSPSEKRKKTFVGEPQRLGSETRLPQHIREATAAP
HGKRKRKKKKRPEDTAASALQEGQTQRQPGSPMYRREGQAQLPAVRRQEDGTQPQVNGQQVGCVTDGHHA
SSRKRRRKGAEGLGEEGGLHQDPLRHSCSPMGDGDPEAMEESPRKKKKKKRKQETQRAVEEDGHLKCPRS
AKPQDAVVPESSSCAPSANGWCPGDRMGLSQAPPVSWNGERESDVVQELLKYSSDKAYGRKVLTWDGKMS
AVSQDAIEDSRQARTETVVDDWDEEFDRGKEKKIKKFKREKRRNFNAFQKLQTRRNFWSVTHPAKAASLS
YRR

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_025090
ORF Size 3369 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_025090.3, NP_079366.3
RefSeq Size 5234 bp
RefSeq ORF 3372 bp
Locus ID 57602
Cytogenetics 17q25.3
Domains UCH
Protein Families Druggable Genome, Protease
MW 122.8 kDa
Gene Summary This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins. The encoded protein may deubiquitinate and stabilize the transcription factor c-Myc, also known as MYC, an important oncoprotein known to be upregulated in most human cancers. The encoded protease may also regulate the activation of autophagy. This gene exhibits elevated expression in some breast and lung cancers. [provided by RefSeq, Mar 2016]
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