USP36 Mouse Monoclonal Antibody [Clone ID: OTI7G3]

CAT#: TA800095

USP36 mouse monoclonal antibody, clone OTI7G3 (formerly 7G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY USP36 (Cat# RC209781, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP36(Cat# TA800095). Positive lysates LY403047 (100ug) and LC403047 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP36 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800095)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-USP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800095)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-USP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800095)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-USP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800095)

• Anti-USP36 mouse monoclonal antibody (TA800095) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY USP36 (RC209781).

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Specifications

Product Data

• Clone Name: OTI7G3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 589-972 of human USP36 (NP_079366) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 122.7 kDa
• Gene Name: ubiquitin specific peptidase 36
• Database Link:
  NP_079366
  Entrez Gene 483344 DogEntrez Gene 57602 Human
  UniProt ID Q9P275
• Background: Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP36 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]). [supplied by OMIM]
• Synonyms: DUB1

Reference Data

• Protein Families: Druggable Genome, Protease