WNT5A (NM_003392) Human Tagged ORF Clone
WNT5A (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 5A (WNT5A)
|Product Name||WNT5A (NM_003392) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC209206 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC209206 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1140 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_003392.3, NM_003392.4, NP_003383.2|
|RefSeq Size||6194 bp|
|RefSeq ORF||1143 bp|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway|
|Protein Pathways||Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway|
|Gene Summary||The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]|
|The use of this cDNA Clones has been cited in the following citations:|
Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells
,Corbett, L;Mann, J;Mann, DA;,
PLoS ONE Nov 2015
,PubMed ID 26566235
Hepatic interferon7stimulated genes are differentially regulated in the liver of chronic hepatitis C patients with different interleukin 28B genotypes
,Honda, M;Shirasaki, T;Shimakami, T;Sakai, A;,
Hepatology DOI: 10.1002/hep.26788
|cDNA Clone Resources|
|SC126838||WNT5A (untagged)-Human wingless-type MMTV integration site family, member 5A (WNT5A)||
|RG209206||WNT5A (GFP-tagged) - Human wingless-type MMTV integration site family, member 5A (WNT5A)||
|RC209206L1||Lenti ORF clone of Human wingless-type MMTV integration site family, member 5A (WNT5A), Myc-DDK-tagged||
|RC209206L2||Lenti ORF clone of Human wingless-type MMTV integration site family, member 5A (WNT5A), mGFP tagged||
|RC209206L3||Lenti ORF clone of Human wingless-type MMTV integration site family, member 5A (WNT5A), Myc-DDK-tagged||
|RC209206L4||Lenti ORF clone of Human wingless-type MMTV integration site family, member 5A (WNT5A), mGFP tagged||