WNT5A (NM_003392) Human Untagged Clone

CAT#: SC126838

WNT5A (untagged)-Human wingless-type MMTV integration site family, member 5A (WNT5A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNT5A
• Synonyms: hWNT5A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126838 sequence for NM_003392 edited (data generated by NextGen Sequencing)
  ATGAAGAAGTCCATTGGAATATTAAGCCCAGGAGTTGCTTTGGGGATGGCTGGAAGTGCA
  ATGTCTTCCAAGTTCTTCCTAGTGGCTTTGGCCATATTTTTCTCCTTCGCCCAGGTTGTA
  ATTGAAGCCAATTCTTGGTGGTCGCTAGGTATGAATAACCCTGTTCAGATGTCAGAAGTA
  TATATTATAGGAGCACAGCCTCTCTGCAGCCAACTGGCAGGACTTTCTCAAGGACAGAAG
  AAACTGTGCCACTTGTATCAGGACCACATGCAGTACATCGGAGAAGGCGCGAAGACAGGC
  ATCAAAGAATGCCAGTATCAATTCCGACATCGAAGGTGGAACTGCAGCACTGTGGATAAC
  ACCTCTGTTTTTGGCAGGGTGATGCAGATAGGCAGCCGCGAGACGGCCTTCACATACGCG
  GTGAGCGCAGCAGGGGTGGTGAACGCCATGAGCCGGGCGTGCCGCGAGGGCGAGCTGTCC
  ACCTGCGGCTGCAGCCGCGCCGCGCGCCCCAAGGACCTGCCGCGGGACTGGCTCTGGGGC
  GGCTGCGGCGACAACATCGACTATGGCTACCGCTTTGCCAAGGAGTTCGTGGACGCCCGC
  GAGCGGGAGCGCATCCACGCCAAGGGCTCCTACGAGAGTGCTCGCATCCTCATGAACCTG
  CACAACAACGAGGCCGGCCGCAGGACGGTGTACAACCTGGCTGATGTGGCCTGCAAGTGC
  CATGGGGTGTCCGGCTCATGTAGCCTGAAGACATGCTGGCTGCAGCTGGCAGACTTCCGC
  AAGGTGGGTGATGCCCTGAAGGAGAAGTACGACAGCGCGGCGGCCATGCGGCTCAACAGC
  CGGGGCAAGTTGGTACAGGTCAACAGCCGCTTCAACTCGCCCACCACACAAGACCTGGTC
  TACATCGACCCCAGCCCTGACTACTGCGTGCGCAATGAGAGCACCGGCTCGCTGGGCACG
  CAGGGCCGCCTGTGCAACAAGACGTCGGAGGGCATGGATGGCTGCGAGCTCATGTGCTGC
  GGCCGTGGCTACGACCAGTTCAAGACCGTGCAGACGGAGCGCTGCCACTGCAAGTTCCAC
  TGGTGCTGCTACGTCAAGTGCAAGAAGTGCACGGAGATCGTGGACCAGTTTGTGTGCAAG
  TAG
  
  Clone variation with respect to NM_003392.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003392 unedited
  GACCGTCCCCCCAGGCTTAACCCGGTCGCTCCGCTCGGATTCCTCGGCTGCGCTCGCTCG
  GGTGGCGACTTCCTCCCCGCGCCCCCTCCCCCTCGCCATGAAGAAGTCCATTGGAATATT
  AAGCCCAGGAGTTGCTTTGGGGATGGCTGGAAGTGCAATGTCTTCCAAGTTCTTCCTAGT
  GGCTTTGGCCATATTTTTCTCCTTCGCCCAGGTTGTAATTGAAGCCAATTCTTGGTGGTC
  GCTAGGTATGAATAACCCTGTTCAGATGTCAGAAGTATATATTATAGGAGCACAGCCTCT
  TCTGCAGCCAACTGGCAGGACTTTCTCAAGGACAGAAGAAACTGTGCCACTTGTATCAAG
  ACCACATGCAGTACATCCGGAGAAGGCGCGAAGACCGGCATCANANAATGCCAGTATCAA
  TTCCNACATCCGAANGTGGAACTGCAGCACTGGGGATAACACCTCCTGTTTTTTGGCAGG
  GTGATGCACATAAGCCAGCCGNGANACGGCCTTTNCATACNCCGTGANGCGCAANAGGGG
  TGGGNGAACNCCNTTNAACCGNGCCGTGCCCNCNAGGGCNAACCTGTCCCCCTTGCGGNT
  GNCANCCGCGGCCGCNNCCCCCAAGGNNCTGCCCCCGGAACTGGTTTTTGGTNGGNTTGT
  GNGCGACCACATCCACTATGGNGTCCGCTTTGCCAAGAAGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003392 unedited
  GGGGTTTATTAGTTACGTGGCCGCAATCGGGGTTTNGTTTTAATCTTTTTTTTTTGATAA
  AATGATTTTATTAGGAAAAGTACAAGATATGAACAAAAATAAATAATCTTTATCTCATTT
  CTAGCCCAGCAAATTGTACACTGCATATAAAAATGGTCTAAGATGCAAGTTTCCTCCATT
  CCTTTTTTGCTTTTAAAATACTGAGACAGCATTTCAATTCAATATTCTAGGTTCAAACTG
  ATACATTAAAAAAAAATCATACCAACCTTTAATCATTCTACATCCATTTTTTAAAGTTAG
  CTAACACGATGATGTTTCACTAAAATAAAATATCCAATCATCAGATTAAAGTGTAAAGTT
  TGCGTGAACAGGGAAATTACATCATTGCTCTAAGTTTTAATTCCTATGTTTCTGAATGTT
  TTTTGAATTAAAAATTCATTTCATCATCTTACTTTCCAAACACGGGTTCTCTCTTTCACC
  ATTCCACAGAGAGAGAAAAGACTAGAAAATACTTTAAAAAAAATAATTATTTCAAAAGTA
  TTACTTACAGTGACCATCGGACACTTTTAGACTCGTGCCTTTGTGCCCATTTGCATTAAC
  TGGCTGCTTCATGTCACTACTACCCCTTCTATCTCTTCCATTTATTCCGGGCCCCCAAGT
  GCCTCGTAGAATGAGGCTGATAACGATTCTTCGCGAGCCAGCTCTTACTCGGCCTTTTAA
  TGAAGCAGATTTACCTCTGGCTTCGCCGCGAACGACGGATACGTCCAGGGGGTTTCGACC
  CGCTTCCGCCAACCTCCATTAACGCCGGGGCTTTGGCCACGTTTTTCCTAGGCCAACGAT
  TCCCGGGAGCGGACCGGAGAGCCNACCCCCCGGTGGCCACCCGGGGGCGCGGGCCCGCCA
  CCACACCCCCCCCCACCACCCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003392
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003392.3, NP_003383.2
• RefSeq Size: 5855 bp
• RefSeq ORF: 1143 bp
• Locus ID: 7474
• UniProt ID: P41221
• Cytogenetics: 3p14.3
• Domains: wnt
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.