PM20D1 (NM_152491) Human Tagged ORF Clone

CAT#: RC209009

PM20D1 (Myc-DDK-tagged)-Human peptidase M20 domain containing 1 (PM20D1)

Product Images

Circular map for RC209009

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  Western blot validation of overexpression lysate (Cat# LY407498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209009 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PM20D1 protein (Cat# TP309009). The protein was produced from HEK293T cells transfected with PM20D1 cDNA clone (Cat# RC209009) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PM20D1
• Synonyms: Cps1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209009 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGCGGTGCGTTTGCGTGCTGGCCCTGGTGGCTATGCTGCTCCTAGTTTTCCCTACCGTCTCCA
  GATCGATGGGCCCGAGGAGCGGGGAGCATCAAAGGGCGTCGCGAATCCCTTCTCAGTTCAGCAAAGAGGA
  ACGCGTCGCGATGAAAGAGGCGCTGAAAGGTGCCATCCAGATTCCAACAGTGACTTTTAGCTCTGAGAAG
  TCCAATACTACAGCCCTGGCTGAGTTCGGAAAATACATTCATAAAGTCTTTCCTACAGTGGTCAGCACCA
  GCTTTATCCAGCATGAAGTCGTGGAAGAGTATAGCCACCTGTTCACTATCCAAGGCTCGGACCCCAGCTT
  GCAGCCCTACCTGCTGATGGCTCACTTTGATGTGGTGCCTGCCCCTGAAGAAGGCTGGGAGGTGCCCCCA
  TTCTCTGGGTTGGAGCGTGATGGCGTCATCTATGGTTGGGGCACACTGGACGACAAGAACTCTGTGATGG
  CATTACTGCAGGCCTTGGAGCTCCTGCTGATCAGGAAGTACATCCCCCGAAGATCTTTCTTCATTTCTCT
  GGGCCATGATGAGGAGTCATCAGGGACAGGGGCTCAGAGGATCTCAGCCCTGCTACAGTCAAGGGGCGTC
  CAGCTAGCCTTCATTGTGGACGAGGGGGGCTTCATCTTGGATGATTTCATTCCTAACTTCAAGAAGCCCA
  TCGCCTTGATTGCAGTCTCAGAGAAGGGTTCCATGAACCTCATGCTGCAAGTAAACATGACTTCAGGCCA
  CTCTTCAGCTCCTCCAAAGGAGACAAGCATTGGCATCCTTGCAGCTGCTGTCAGCCGATTGGAGCAGACA
  CCAATGCCTATCATATTTGGAAGCGGGACAGTGGTGACTGTATTGCAGCAACTGGCAAATGAGTTTCCCT
  TCCCTGTCAATATAATCCTGAGCAACCCATGGCTATTTGAACCACTTATAAGCAGGTTTATGGAGAGAAA
  TCCCTTAACCAATGCAATAATCAGGACCACCACGGCACTCACCATATTCAAAGCAGGGGTCAAGTTCAAT
  GTCATCCCCCCAGTGGCCCAGGCCACAGTCAACTTCCGGATTCACCCTGGACAGACAGTCCAAGAGGTCC
  TAGAACTCACGAAGAACATTGTGGCTGATAACAGAGTCCAGTTCCATGTGTTGAGTGCCTTTGACCCCCT
  CCCCGTCAGCCCTTCTGATGACAAGGCCTTGGGCTACCAGCTGCTCCGCCAGACCGTACAGTCCGTCTTC
  CCGGAAGTCAATATTACTGCCCCAGTTACTTCTATTGGCAACACAGACAGCCGATTCTTTACAAACCTCA
  CCACTGGCATCTACAGGTTCTACCCCATCTACATACAGCCTGAAGACTTCAAACGCATCCATGGAGTCAA
  CGAGAAAATCTCAGTCCAAGCCTATGAGACCCAAGTGAAATTCATCTTTGAGTTGATTCAGAATGCTGAC
  ACAGACCAGGAGCCAGTTTCTCACCTGCACAAACTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209009 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQRCVCVLALVAMLLLVFPTVSRSMGPRSGEHQRASRIPSQFSKEERVAMKEALKGAIQIPTVTFSSEK
  SNTTALAEFGKYIHKVFPTVVSTSFIQHEVVEEYSHLFTIQGSDPSLQPYLLMAHFDVVPAPEEGWEVPP
  FSGLERDGVIYGWGTLDDKNSVMALLQALELLLIRKYIPRRSFFISLGHDEESSGTGAQRISALLQSRGV
  QLAFIVDEGGFILDDFIPNFKKPIALIAVSEKGSMNLMLQVNMTSGHSSAPPKETSIGILAAAVSRLEQT
  PMPIIFGSGTVVTVLQQLANEFPFPVNIILSNPWLFEPLISRFMERNPLTNAIIRTTTALTIFKAGVKFN
  VIPPVAQATVNFRIHPGQTVQEVLELTKNIVADNRVQFHVLSAFDPLPVSPSDDKALGYQLLRQTVQSVF
  PEVNITAPVTSIGNTDSRFFTNLTTGIYRFYPIYIQPEDFKRIHGVNEKISVQAYETQVKFIFELIQNAD
  TDQEPVSHLHKL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152491
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152491.2
• RefSeq Size: 2200 bp
• RefSeq ORF: 1509 bp
• Locus ID: 148811
• UniProt ID: Q6GTS8
• Cytogenetics: 1q32.1
• Protein Families: Transmembrane
• MW: 55.8 kDa
• Gene Summary: Bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. Thereby, this secreted protein may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.[UniProtKB/Swiss-Prot Function]