CPNE2 (NM_152727) Human Tagged ORF Clone

CAT#: RC208830

CPNE2 (Myc-DDK-tagged)-Human copine II (CPNE2)

Product Images

Circular map for RC208830

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  Western blot validation of overexpression lysate (Cat# LY407324) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208830 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CPNE2 protein (Cat# TP308830). The protein was produced from HEK293T cells transfected with CPNE2 cDNA clone (Cat# RC208830) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPNE2
• Synonyms: COPN2; CPN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208830 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACATACCCAGTGGGGGTGCCCCAGCAGCGGGGGCAGCCCCCATGGGCCCCCAGTATTGCGTGT
  GCAAGGTGGAGCTGTCAGTGAGTGGCCAGAACCTACTGGACCGGGATGTTACCTCCAAGTCCGACCCCTT
  CTGTGTCCTCTTTACAGAGAACAATGGCAGATGGATCGAGTACGACAGGACAGAAACCGCGATCAACAAC
  CTCAACCCCGCCTTCTCCAAGAAGTTCGTGCTTGACTACCACTTCGAGGAGGTACAGAAGCTCAAGTTCG
  CGCTCTTTGACCAGGACAAGTCCAGTATGCGGCTGGACGAGCATGACTTCCTGGGCCAGTTCTCCTGCAG
  CCTGGGCACGATCGTCTCCAGCAAGAAGATCACTAGGCCTCTGCTGCTGCTGAATGACAAGCCTGCGGGG
  AAGGGCTTGATTACGATCGCTGCCCAGGAGCTGTCCGACAACCGCGTCATCACACTAAGCCTGGCGGGCA
  GGAGGCTGGACAAGAAGGACCTCTTTGGGAAGTCAGACCCCTTTCTGGAGTTTTATAAGCCAGGAGACGA
  TGGCAAGTGGATGCTGGTCCACAGGACTGAGGTGATCAAGTACACACTGGACCCTGTGTGGAAGCCATTC
  ACAGTGCCCTTGGTGTCCCTGTGTGATGGGGACATGGAGAAGCCCATCCAGGTCATGTGCTACGACTATG
  ACAATGACGGGGGCCATGACTTCATCGGCGAGTTCCAGACCTCAGTGTCACAGATGTGTGAGGCTCGAGA
  CAGCGTCCCGCTGGAGTTCGAGTGCATCAACCCCAAGAAGCAGAGGAAGAAGAAGAACTATAAAAACTCG
  GGCATCATCATCCTGCGATCCTGCAAGATAAACCGAGACTACTCCTTCCTTGACTACATCCTGGGAGGCT
  GCCAGCTCATGTTCACCGTTGGAATAGACTTTACAGCCTCCAACGGGAATCCCCTCGACCCTTCCTCTTT
  GCACTATATCAACCCTATGGGCACCAACGAATATCTGTCGGCCATCTGGGCTGTTGGGCAGATCATTCAG
  GACTACGACAGTGATAAGATGTTTCCAGCTCTGGGATTCGGGGCCCAGTTACCCCCAGACTGGAAGGTCT
  CCCATGAGTTTGCCATCAACTTCAACCCCACCAACCCCTTCTGCTCAGGTGTGGATGGTATTGCCCAGGC
  GTACTCAGCTTGCCTGCCCCACATCCGCTTCTACGGTCCTACCAATTTCTCCCCCATCGTCAACCACGTG
  GCCCGGTTTGCGGCCCAGGCCACACAACAGCGGACGGCCACGCAGTACTTCATCCTCCTCATCATCACGG
  ACGGGGTCATCAGTGACATGGAGGAGACACGGCATGCCGTGGTGCAGGCTTCCAAGCTGCCCATGTCCAT
  CATCATCGTGGGCGTGGGCAATGCGGACTTCGCTGCCATGGAGTTCCTGGATGGGGACAGCCGCATGCTG
  CGCTCCCACACGGGGGAGGAGGCAGCCCGCGATATTGTGCAGTTCGTTCCCTTTCGAGAGTTCCGCAACG
  CAGCAAAAGAGACCTTGGCCAAAGCTGTGCTGGCGGAGCTGCCCCAACAAGTTGTGCAGTATTTCAAGCA
  TAAAAACCTGCCCCCCACCAACTCGGAGCCCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208830 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHIPSGGAPAAGAAPMGPQYCVCKVELSVSGQNLLDRDVTSKSDPFCVLFTENNGRWIEYDRTETAINN
  LNPAFSKKFVLDYHFEEVQKLKFALFDQDKSSMRLDEHDFLGQFSCSLGTIVSSKKITRPLLLLNDKPAG
  KGLITIAAQELSDNRVITLSLAGRRLDKKDLFGKSDPFLEFYKPGDDGKWMLVHRTEVIKYTLDPVWKPF
  TVPLVSLCDGDMEKPIQVMCYDYDNDGGHDFIGEFQTSVSQMCEARDSVPLEFECINPKKQRKKKNYKNS
  GIIILRSCKINRDYSFLDYILGGCQLMFTVGIDFTASNGNPLDPSSLHYINPMGTNEYLSAIWAVGQIIQ
  DYDSDKMFPALGFGAQLPPDWKVSHEFAINFNPTNPFCSGVDGIAQAYSACLPHIRFYGPTNFSPIVNHV
  ARFAAQATQQRTATQYFILLIITDGVISDMEETRHAVVQASKLPMSIIIVGVGNADFAAMEFLDGDSRML
  RSHTGEEAARDIVQFVPFREFRNAAKETLAKAVLAELPQQVVQYFKHKNLPPTNSEPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152727
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152727.6
• RefSeq Size: 2252 bp
• RefSeq ORF: 1647 bp
• Locus ID: 221184
• UniProt ID: Q96FN4
• Cytogenetics: 16q13
• Domains: C2
• MW: 61.2 kDa
• Gene Summary: Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene is one of several genes that encode a calcium-dependent protein containing two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. Sequence analysis identified multiple alternatively spliced transcript variants but their full-length natures could not be determined. [provided by RefSeq, Jul 2008]