METT10D (METTL16) (NM_024086) Human Tagged ORF Clone

CAT#: RC208648

METTL16 (Myc-DDK-tagged)-Human methyltransferase like 16 (METTL16)

Product Images

Circular map for RC208648

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY METT10D (Cat# RC208648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METT10D(Cat# TA504710). Positive lysates LY411358 (100ug) and LC411358 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411358) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208648 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified METTL16 protein (Cat# TP308648). The protein was produced from HEK293T cells transfected with METTL16 cDNA clone (Cat# RC208648) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: METT10D
• Synonyms: METT10D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208648 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAGTAAATCAATGCATGCAAGAAATAGATACAAGGACAAACCTCCTGACTTTGCATATCTGG
  CATCCAAATATCCAGATTTTAAGCAGCATGTTCAGATAAATCTGAATGGAAGAGTGAGCCTTAATTTTAA
  AGACCCCGAAGCAGTCAGAGCTCTGACGTGTACTCTCCTAAGGGAAGATTTTGGACTTTCTATTGATATT
  CCATTGGAGAGACTAATTCCCACAGTTCCCTTGAGACTCAACTATATTCACTGGGTAGAAGATCTGATCG
  GTCACCAGGATTCTGACAAAAGTACTCTCCGAAGAGGAATTGACATAGGCACGGGGGCATCTTGCATCTA
  CCCCTTACTTGGAGCAACCTTGAATGGCTGGTATTTCCTCGCAACAGAAGTGGATGATATGTGTTTCAAC
  TATGCAAAGAAAAATGTGGAACAGAATAACTTATCTGATCTCATAAAAGTGGTGAAAGTGCCACAGAAGA
  CACTCCTGATGGATGCTCTTAAAGAAGAATCTGAGATAATCTATGACTTTTGCATGTGCAACCCTCCCTT
  TTTTGCCAATCAATTGGAAGCCAAGGGAGTAAACTCACGAAATCCTCGAAGACCTCCGCCTAGTTCTGTT
  AATACAGGAGGCATCACAGAGATCATGGCAGAAGGAGGTGAATTAGAGTTTGTTAAAAGGATCATCCATG
  ACAGTCTACAACTTAAAAAAAGATTAAGATGGTATAGCTGCATGCTGGGAAAGAAATGCAGCCTGGCGCC
  TCTGAAGGAGGAGCTTCGCATACAAGGGGTTCCCAAAGTAACGTACACTGAATTCTGTCAAGGTCGGACA
  ATGAGATGGGCCTTAGCTTGGAGTTTTTATGATGATGTCACAGTACCATCACCACCAAGTAAGCGAAGAA
  AATTAGAGAAACCGAGAAAACCCATAACATTCGTGGTGCTGGCGTCCGTGATGAAGGAATTATCCCTCAA
  AGCATCACCTCTGCGCTCGGAGACGGCGGAAGGCATAGTCGTTGTCACGACATGGATTGAAAAAATTCTC
  ACTGATTTGAAGGTCCAGCATAAACGAGTTCCCTGTGGAAAAGAGGAAGTCAGCCTTTTCCTAACGGCCA
  TAGAAAACTCCTGGATTCATTTAAGGAGAAAGAAAAGAGAGCGTGTGAGACAGCTGAGAGAAGTTCCCCG
  AGCTCCTGAGGACGTCATTCAGGCCTTGGAAGAGAAAAAGCCCACCCCCAAAGAGTCTGGCAATAGCCAA
  GAACTGGCCAGGGGCCCCCAGGAGAGGACCCCCTGTGGGCCTGCTCTGCGGGAAGGCGAGGCTGCCGCTG
  TGGAGGGCCCGTGCCCGAGCCAGGAGTCCCTGTCCCAGGAGGAAAACCCGGAACCCACGGAGGATGAAAG
  GAGTGAGGAAAAGGGAGGGGTGGAGGTTTTGGAAAATTGTCAAGGCTCTAGCAACGGAGCCCAGGACCAA
  GAGGCTTCTGAGCAGTTCGGCAGCCCAGTGGCTGAAAGGGGGAAACGTCTCCCAGGAGTGGCCGGACAGT
  ACCTGTTTAAGTGTTTGATAAACGTTAAGAAGGAGGTGGACGATGCCTTAGTGGAGATGCACTGGGTTGA
  GGGCCAGAACAGGGATCTGATGAACCAGCTTTGCACCTACATACGTAACCAAATTTTCAGGCTTGTTGCA
  GTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208648 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSKSMHARNRYKDKPPDFAYLASKYPDFKQHVQINLNGRVSLNFKDPEAVRALTCTLLREDFGLSIDI
  PLERLIPTVPLRLNYIHWVEDLIGHQDSDKSTLRRGIDIGTGASCIYPLLGATLNGWYFLATEVDDMCFN
  YAKKNVEQNNLSDLIKVVKVPQKTLLMDALKEESEIIYDFCMCNPPFFANQLEAKGVNSRNPRRPPPSSV
  NTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSCMLGKKCSLAPLKEELRIQGVPKVTYTEFCQGRT
  MRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLASVMKELSLKASPLRSETAEGIVVVTTWIEKIL
  TDLKVQHKRVPCGKEEVSLFLTAIENSWIHLRRKKRERVRQLREVPRAPEDVIQALEEKKPTPKESGNSQ
  ELARGPQERTPCGPALREGEAAAVEGPCPSQESLSQEENPEPTEDERSEEKGGVEVLENCQGSSNGAQDQ
  EASEQFGSPVAERGKRLPGVAGQYLFKCLINVKKEVDDALVEMHWVEGQNRDLMNQLCTYIRNQIFRLVA
  VN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024086
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024086.3, NP_076991.3
• RefSeq Size: 5758 bp
• RefSeq ORF: 1689 bp
• Locus ID: 79066
• UniProt ID: Q86W50
• Cytogenetics: 17p13.3
• MW: 63.6 kDa
• Gene Summary: RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3' UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3' UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200). Also able to bind various lncRNAs (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).[UniProtKB/Swiss-Prot Function]