METT10D (METTL16) (NM_024086) Human Tagged ORF Clone

CAT#: RC208648

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  • TrueORF®

METTL16 (Myc-DDK-tagged)-Human methyltransferase like 16 (METTL16)



  "NM_024086" in other vectors (5)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol METTL16
Synonyms METT10D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208648 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGAGTAAATCAATGCATGCAAGAAATAGATACAAGGACAAACCTCCTGACTTTGCATATCTGG
CATCCAAATATCCAGATTTTAAGCAGCATGTTCAGATAAATCTGAATGGAAGAGTGAGCCTTAATTTTAA
AGACCCCGAAGCAGTCAGAGCTCTGACGTGTACTCTCCTAAGGGAAGATTTTGGACTTTCTATTGATATT
CCATTGGAGAGACTAATTCCCACAGTTCCCTTGAGACTCAACTATATTCACTGGGTAGAAGATCTGATCG
GTCACCAGGATTCTGACAAAAGTACTCTCCGAAGAGGAATTGACATAGGCACGGGGGCATCTTGCATCTA
CCCCTTACTTGGAGCAACCTTGAATGGCTGGTATTTCCTCGCAACAGAAGTGGATGATATGTGTTTCAAC
TATGCAAAGAAAAATGTGGAACAGAATAACTTATCTGATCTCATAAAAGTGGTGAAAGTGCCACAGAAGA
CACTCCTGATGGATGCTCTTAAAGAAGAATCTGAGATAATCTATGACTTTTGCATGTGCAACCCTCCCTT
TTTTGCCAATCAATTGGAAGCCAAGGGAGTAAACTCACGAAATCCTCGAAGACCTCCGCCTAGTTCTGTT
AATACAGGAGGCATCACAGAGATCATGGCAGAAGGAGGTGAATTAGAGTTTGTTAAAAGGATCATCCATG
ACAGTCTACAACTTAAAAAAAGATTAAGATGGTATAGCTGCATGCTGGGAAAGAAATGCAGCCTGGCGCC
TCTGAAGGAGGAGCTTCGCATACAAGGGGTTCCCAAAGTAACGTACACTGAATTCTGTCAAGGTCGGACA
ATGAGATGGGCCTTAGCTTGGAGTTTTTATGATGATGTCACAGTACCATCACCACCAAGTAAGCGAAGAA
AATTAGAGAAACCGAGAAAACCCATAACATTCGTGGTGCTGGCGTCCGTGATGAAGGAATTATCCCTCAA
AGCATCACCTCTGCGCTCGGAGACGGCGGAAGGCATAGTCGTTGTCACGACATGGATTGAAAAAATTCTC
ACTGATTTGAAGGTCCAGCATAAACGAGTTCCCTGTGGAAAAGAGGAAGTCAGCCTTTTCCTAACGGCCA
TAGAAAACTCCTGGATTCATTTAAGGAGAAAGAAAAGAGAGCGTGTGAGACAGCTGAGAGAAGTTCCCCG
AGCTCCTGAGGACGTCATTCAGGCCTTGGAAGAGAAAAAGCCCACCCCCAAAGAGTCTGGCAATAGCCAA
GAACTGGCCAGGGGCCCCCAGGAGAGGACCCCCTGTGGGCCTGCTCTGCGGGAAGGCGAGGCTGCCGCTG
TGGAGGGCCCGTGCCCGAGCCAGGAGTCCCTGTCCCAGGAGGAAAACCCGGAACCCACGGAGGATGAAAG
GAGTGAGGAAAAGGGAGGGGTGGAGGTTTTGGAAAATTGTCAAGGCTCTAGCAACGGAGCCCAGGACCAA
GAGGCTTCTGAGCAGTTCGGCAGCCCAGTGGCTGAAAGGGGGAAACGTCTCCCAGGAGTGGCCGGACAGT
ACCTGTTTAAGTGTTTGATAAACGTTAAGAAGGAGGTGGACGATGCCTTAGTGGAGATGCACTGGGTTGA
GGGCCAGAACAGGGATCTGATGAACCAGCTTTGCACCTACATACGTAACCAAATTTTCAGGCTTGTTGCA
GTTAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208648 protein sequence
Red=Cloning site Green=Tags(s)

MALSKSMHARNRYKDKPPDFAYLASKYPDFKQHVQINLNGRVSLNFKDPEAVRALTCTLLREDFGLSIDI
PLERLIPTVPLRLNYIHWVEDLIGHQDSDKSTLRRGIDIGTGASCIYPLLGATLNGWYFLATEVDDMCFN
YAKKNVEQNNLSDLIKVVKVPQKTLLMDALKEESEIIYDFCMCNPPFFANQLEAKGVNSRNPRRPPPSSV
NTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSCMLGKKCSLAPLKEELRIQGVPKVTYTEFCQGRT
MRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLASVMKELSLKASPLRSETAEGIVVVTTWIEKIL
TDLKVQHKRVPCGKEEVSLFLTAIENSWIHLRRKKRERVRQLREVPRAPEDVIQALEEKKPTPKESGNSQ
ELARGPQERTPCGPALREGEAAAVEGPCPSQESLSQEENPEPTEDERSEEKGGVEVLENCQGSSNGAQDQ
EASEQFGSPVAERGKRLPGVAGQYLFKCLINVKKEVDDALVEMHWVEGQNRDLMNQLCTYIRNQIFRLVA
VN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024086
ORF Size 1686 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024086.3, NP_076991.3
RefSeq Size 5758 bp
RefSeq ORF 1689 bp
Locus ID 79066
UniProt ID Q86W50
Cytogenetics 17p13.3
MW 63.6 kDa
Gene Summary RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3' UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3' UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200). Also able to bind various lncRNAs (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).[UniProtKB/Swiss-Prot Function]

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