RAB17 (NM_022449) Human Tagged ORF Clone

CAT#: RC208378

RAB17 (Myc-DDK-tagged)-Human RAB17, member RAS oncogene family (RAB17), transcript variant 1

Product Images

Circular map for RC208378

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RAB17 (Cat# RC208378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB17(Cat# TA501239). Positive lysates LY402920 (100ug) and LC402920 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402920) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208378 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RAB17 protein (Cat# TP308378). The protein was produced from HEK293T cells transfected with RAB17 cDNA clone (Cat# RC208378) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAB17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208378 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGGCACACAGGACCCCCCAGCCCAGGGCTGCCCCCAGCCAGCCCCGTGTGTTCAAGCTGGTTC
  TCCTGGGAAGTGGCTCCGTGGGTAAGTCCAGCTTGGCTCTTCGGTACGTGAAGAACGACTTCAAGAGTAT
  CCTGCCTACGGTGGGCTGTGCGTTCTTCACAAAGGTGGTGGATGTGGGTGCCACCTCTCTGAAGCTTGAG
  ATCTGGGACACAGCTGGCCAGGAGAAGTACCACAGCGTCTGCCACCTCTACTTCAGGGGTGCCAACGCTG
  CGCTTCTGGTGTACGACATCACCAGGAAGGATTCCTTCCTCAAGGCTCAGCAGTGGCTGAAGGACCTGGA
  GGAGGAGCTGCACCCAGGAGAAGTCCTGGTGATGCTGGTGGGCAACAAGACGGACCTCAGCCAGGAGCGG
  GAGGTGACCTTCCAGGAAGGGAAGGAGTTTGCCGACAGCCAGAAGTTGCTGTTCATGGAAACTTCGGCCA
  AACTGAACCACCAGGTGTCGGAGGTGTTCAATACAGTGGCCCAAGAGCTACTGCAGAGAAGCGACGAGGA
  GGGCCAGGCTCTACGGGGGGATGCAGCTGTGGCTCTGAACAAGGGGCCCGCGAGGCAGGCCAAATGCTGC
  GCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208378 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQAHRTPQPRAAPSQPRVFKLVLLGSGSVGKSSLALRYVKNDFKSILPTVGCAFFTKVVDVGATSLKLE
  IWDTAGQEKYHSVCHLYFRGANAALLVYDITRKDSFLKAQQWLKDLEEELHPGEVLVMLVGNKTDLSQER
  EVTFQEGKEFADSQKLLFMETSAKLNHQVSEVFNTVAQELLQRSDEEGQALRGDAAVALNKGPARQAKCC
  AH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022449
• ORF Size: 636 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022449.4
• RefSeq Size: 2018 bp
• RefSeq ORF: 639 bp
• Locus ID: 64284
• UniProt ID: Q9H0T7
• Cytogenetics: 2q37.3
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• MW: 23.5 kDa
• Gene Summary: The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase (Lutcke et al., 1993 [PubMed 8486736]).[supplied by OMIM, Oct 2009]