PCYT1A (NM_005017) Human Tagged ORF Clone

CAT#: RC208130

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PCYT1A (Myc-DDK-tagged)-Human phosphate cytidylyltransferase 1, choline, alpha (PCYT1A)



  "NM_005017" in other vectors (6)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PCYT1A
Synonyms CCTA; CCTalpha; CT; CTA; CTPCT; PCYT1; SMDCRD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208130 representing NM_005017
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCACAGTGTTCAGCCAAGGTCAATGCAAGGAAGAGGAGAAAAGAGGCGCCCGGACCCAACGGGG
CAACAGAAGAAGATGGGGTTCCTTCCAAAGTGCAGCGCTGTGCAGTGGGCTTACGGCAACCAGCTCCTTT
TTCTGATGAAATTGAAGTTGACTTTAGTAAGCCCTATGTCAGGGTAACTATGGAAGAAGCCAGCAGAGGA
ACTCCTTGTGAGCGACCTGTGAGAGTTTATGCCGATGGAATATTTGACTTATTTCACTCTGGTCACGCCC
GAGCTCTGATGCAAGCGAAGAACCTTTTCCCTAATACGTACCTCATTGTGGGAGTTTGCAGTGATGAGCT
CACACACAACTTCAAAGGCTTCACGGTGATGAACGAGAATGAGCGCTATGACGCAGTCCAGCACTGCCGC
TACGTGGATGAGGTGGTGAGGAATGCGCCCTGGACGCTGACACCCGAGTTCCTGGCCGAACACCGGATTG
ATTTTGTAGCCCATGATGATATTCCTTATTCATCTGCTGGCAGTGATGATGTTTATAAGCACATCAAGGA
GGCAGGCATGTTTGCTCCAACACAGAGGACAGAAGGTATCTCCACATCAGACATCATCACCCGAATTGTG
CGGGATTATGATGTGTATGCGAGGCGGAACCTGCAGAGGGGCTACACAGCAAAGGAGCTCAATGTCAGCT
TTATCAACGAGAAGAAATACCACTTGCAGGAGAGGGTTGACAAAGTAAAGAAGAAAGTGAAAGATGTGGA
GGAAAAGTCAAAAGAATTTGTTCAGAAGGTGGAGGAAAAAAGCATTGACCTCATTCAGAAGTGGGAGGAG
AAGTCCCGAGAATTCATTGGAAGTTTTCTGGAAATGTTTGGTCCGGAAGGAGCACTGAAACATATGCTGA
AAGAGGGGAAGGGCCGGATGCTGCAGGCCATCAGCCCGAAGCAGAGCCCCAGCAGCAGCCCTACTCGCGA
GCGCTCCCCCTCCCCCTCTTTCCGATGGCCCTTCTCCGGCAAGACTTCCCCACCTTGCTCCCCAGCAAAT
CTCTCCAGGCACAAGGCTGCAGCCTATGATATCAGTGAGGATGAAGAAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208130 representing NM_005017
Red=Cloning site Green=Tags(s)

MDAQCSAKVNARKRRKEAPGPNGATEEDGVPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEASRG
TPCERPVRVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCR
YVDEVVRNAPWTLTPEFLAEHRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIV
RDYDVYARRNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEE
KSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTRERSPSPSFRWPFSGKTSPPCSPAN
LSRHKAAAYDISEDEED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005017
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005017.4
RefSeq Size 1677 bp
RefSeq ORF 1104 bp
Locus ID 5130
UniProt ID P49585
Cytogenetics 3q29
Domains CTP_transf_2
Protein Pathways Glycerophospholipid metabolism, Metabolic pathways
MW 41.6 kDa
Gene Summary This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]

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