POPDC2 (NM_022135) Human Tagged ORF Clone

CAT#: RC208106

POPDC2 (Myc-DDK-tagged)-Human popeye domain containing 2 (POPDC2)

Product Images

Circular map for RC208106

• 

  Western blot validation of overexpression lysate (Cat# LY411755) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208106 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POPDC2
• Synonyms: POP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208106 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCAACAGCAGCAGAGTGGGCCAGCTTCTCTTGCAGGGTTCAGCGTGCATTAGGTGGAAGCAGG
  ATGTGGAAGGGGCTGTCTACCACCTAGCCAACTGCCTCTTACTCCTGGGCTTCATGGGGGGCAGTGGGGT
  GTATGGATGCTTCTATCTTTTTGGCTTCCTGAGTGCAGGTTACCTGTGCTGCGTGCTGTGGGGCTGGTTC
  AGTGCCTGTGGCCTGGACATTGTTCTTTGGAGCTTCCTGCTGGCTGTGGTCTGCCTGCTCCAGCTGGCAC
  ACCTGGTATACCGCCTGCGTGAGGACACCCTCCCTGAGGAGTTTGACCTCCTCTACAAGACGCTGTGCCT
  GCCCTTGCAGGTGCCCCTACAGACATACAAGGAGATTGTTCACTGCTGCGAGGAGCAGGTCTTAACTCTG
  GCCACTGAACAGACCTATGCTGTGGAGGGTGAGACACCCATCAACCGCCTGTCCCTGCTGCTCTCTGGCC
  GGGTTCGTGTGAGCCAGGATGGGCAGTTTCTGCACTACATCTTTCCATACCAGTTCATGGACTCTCCTGA
  GTGGGAATCACTACAGCCTTCTGAGGAGGGGGTGTTCCAGGTCACTCTGACTGCTGAGACCTCATGTAGC
  TACATTTCCTGGCCCCGGAAAAGTCTCCATCTTCTTCTGACCAAAGAGCGATACATCTCCTGCCTCTTCT
  CGGCTCTGCTGGGATATGACATCTCGGAGAAGCTCTACACTCTCAATGACAAGCTCTTTGCTAAGTTTGG
  GCTGCGCTTTGACATCCGCCTTCCCAGCCTCTACCATGTCCTGGGTCCCACTGCTGCAGATGCTGGACCA
  GAGTCCGAGAAGGGTGATGAGGAAGTCTGTGAGCCAGCTGTGTCCCCTCCTCAGGCCACACCCACCTCTC
  TCCAGCAAACACCCCCTTGTTCTACCCCTCCAGCTACCACCAACTTTCCTGCACCTCCTACCCGGGCCAG
  GTTGTCCAGGCCAGACAGTGGCATACTGGCTTCTAGAATTCCTCTCCAGAGCTACTCTCAAGTTATATCC
  AGGGGACAGGCCCCTTTGGCTCCAACCCACACGCCTGAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208106 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSANSSRVGQLLLQGSACIRWKQDVEGAVYHLANCLLLLGFMGGSGVYGCFYLFGFLSAGYLCCVLWGWF
  SACGLDIVLWSFLLAVVCLLQLAHLVYRLREDTLPEEFDLLYKTLCLPLQVPLQTYKEIVHCCEEQVLTL
  ATEQTYAVEGETPINRLSLLLSGRVRVSQDGQFLHYIFPYQFMDSPEWESLQPSEEGVFQVTLTAETSCS
  YISWPRKSLHLLLTKERYISCLFSALLGYDISEKLYTLNDKLFAKFGLRFDIRLPSLYHVLGPTAADAGP
  ESEKGDEEVCEPAVSPPQATPTSLQQTPPCSTPPATTNFPAPPTRARLSRPDSGILASRIPLQSYSQVIS
  RGQAPLAPTHTPEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022135
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022135.4
• RefSeq Size: 1667 bp
• RefSeq ORF: 1095 bp
• Locus ID: 64091
• UniProt ID: Q9HBU9
• Cytogenetics: 3q13.33
• Protein Families: Transmembrane
• MW: 40.4 kDa
• Gene Summary: This gene encodes a member of the POP family of proteins which contain three putative transmembrane domains. This membrane associated protein is predominantly expressed in skeletal and cardiac muscle, and may have an important function in these tissues. [provided by RefSeq, Jul 2008]