SNPH (NM_014723) Human Tagged ORF Clone

CAT#: RC207749

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SNPH (Myc-DDK-tagged)-Human syntaphilin (SNPH)



  "NM_014723" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SNPH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207749 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATGTCCCTGCCAGGAAGTAGACGGACCTCTGCTGGATCACGCAGGCGCACCTCTCCACCTGTGA
GCGTGCGGGATGCCTACGGCACCTCTTCGCTCAGCAGCAGCAGCAATTCTGGCTCCTACAAGGGCAGTGA
CAGCAGTCCCACGCCAAGGCGCTCCATGAAATACACGCTGTGCAGTGACAACCATGGCATCAAGCCCCCG
ACCCCGGAGCAGTACCTGACCCCCCTGCAGCAGAAGGAGGTGTGCATCCGGCACCTGAAAGCCCGGCTGA
AGGACACACAGGACCGGCTCCAGGACCGGGACACAGAGATTGATGACCTGAAGACGCAGCTGTCACGCAT
GCAGGAGGACTGGATTGAGGAGGAGTGCCACCGCGTGGAGGCCCAGCTGGCCCTGAAGGAGGCCCGAAAG
GAGATCAAGCAGCTCAAGCAGGTCATCGACACTGTCAAGAACAACCTGATTGACAAGGACAAGGGGCTGC
AGAAGTACTTCGTGGACATCAACATCCAGAACAAGAAGCTGGAGACGCTGCTGCACAGCATGGAGGTGGC
CCAGAATGGCATGGCCAAGGAGGATGGCACTGGGGAGTCAGCCGGTGGGTCCCCTGCCCGCTCCCTCACC
CGCAGCTCCACCTACACCAAGCTGAGTGACCCGGCTGTCTGTGGTGACCGCCAGCCGGGTGATCCCTCCA
GCGGCTCTGCTGAGGATGGGGCAGACAGTGGCTTTGCAGCAGCCGATGACACACTGAGCCGGACGGACGC
GCTGGAAGCCAGCAGCCTGCTGTCGTCGGGGGTGGACTGTGGCACCGAGGAGACCTCGCTGCACAGCTCC
TTCGGCCTGGGCCCCCGCTTCCCTGCCAGCAACACCTATGAGAAGCTGCTGTGTGGCATGGAGGCTGGTG
TGCAGGCCAGCTGCATGCAGGAGCGTGCCATCCAGACAGACTTCGTGCAGTACCAGCCTGACCTTGACAC
CATCCTGGAGAAAGTGACCCAGGCCCAGGTCTGTGGGACAGACCCTGAGTCAGGGGACAGGTGCCCAGAG
CTGGATGCCCACCCTTCAGGGCCCAGAGACCCCAACTCAGCAGTGGTGGTGACAGTGGGTGATGAGCTAG
AGGCCCCAGAGCCCATCACCCGTGGACCCACCCCACAGCGGCCTGGTGCCAACCCCAACCCTGGCCAGTC
GGTGAGCGTGGTGTGCCCCATGGAAGAGGAGGAGGAGGCTGCCGTGGCTGAGAAGGAGCCCAAGAGCTAC
TGGAGCCGCCACTACATCGTGGATCTGCTGGCTGTGGTGGTGCCGGCCGTGCCCACGGTGGCCTGGCTTT
GCCGCTCCCAGCGGCGCCAGGGCCAGCCCATCTACAACATCAGCTCCCTGCTGCGGGGCTGCTGCACTGT
GGCCTTGCACTCCATCCGCAGGATCAGCTGCCGCTCGCTGAGCCAGCCGAGTCCCAGCCCAGCGGGCGGC
GGCTCCCAGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207749 protein sequence
Red=Cloning site Green=Tags(s)

MAMSLPGSRRTSAGSRRRTSPPVSVRDAYGTSSLSSSSNSGSYKGSDSSPTPRRSMKYTLCSDNHGIKPP
TPEQYLTPLQQKEVCIRHLKARLKDTQDRLQDRDTEIDDLKTQLSRMQEDWIEEECHRVEAQLALKEARK
EIKQLKQVIDTVKNNLIDKDKGLQKYFVDINIQNKKLETLLHSMEVAQNGMAKEDGTGESAGGSPARSLT
RSSTYTKLSDPAVCGDRQPGDPSSGSAEDGADSGFAAADDTLSRTDALEASSLLSSGVDCGTEETSLHSS
FGLGPRFPASNTYEKLLCGMEAGVQASCMQERAIQTDFVQYQPDLDTILEKVTQAQVCGTDPESGDRCPE
LDAHPSGPRDPNSAVVVTVGDELEAPEPITRGPTPQRPGANPNPGQSVSVVCPMEEEEEAAVAEKEPKSY
WSRHYIVDLLAVVVPAVPTVAWLCRSQRRQGQPIYNISSLLRGCCTVALHSIRRISCRSLSQPSPSPAGG
GSQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014723
ORF Size 1482 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014723.4
RefSeq Size 5026 bp
RefSeq ORF 1485 bp
Locus ID 9751
UniProt ID O15079
Cytogenetics 20p13
MW 53.5 kDa
Gene Summary Syntaxin-1, synaptobrevin/VAMP, and SNAP25 interact to form the SNARE complex, which is required for synaptic vesicle docking and fusion. The protein encoded by this gene is membrane-associated and inhibits SNARE complex formation by binding free syntaxin-1. Expression of this gene appears to be brain-specific. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

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