SNPH (NM_014723) Human Tagged ORF Clone

CAT#: RC207749

SNPH (Myc-DDK-tagged)-Human syntaphilin (SNPH)

Product Images

Western blot validation of overexpression lysate (Cat# LY415096) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207749 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SNPH protein (Cat# TP307749). The protein was produced from HEK293T cells transfected with SNPH cDNA clone (Cat# RC207749) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNPH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207749 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGTCCCTGCCAGGAAGTAGACGGACCTCTGCTGGATCACGCAGGCGCACCTCTCCACCTGTGA
  GCGTGCGGGATGCCTACGGCACCTCTTCGCTCAGCAGCAGCAGCAATTCTGGCTCCTACAAGGGCAGTGA
  CAGCAGTCCCACGCCAAGGCGCTCCATGAAATACACGCTGTGCAGTGACAACCATGGCATCAAGCCCCCG
  ACCCCGGAGCAGTACCTGACCCCCCTGCAGCAGAAGGAGGTGTGCATCCGGCACCTGAAAGCCCGGCTGA
  AGGACACACAGGACCGGCTCCAGGACCGGGACACAGAGATTGATGACCTGAAGACGCAGCTGTCACGCAT
  GCAGGAGGACTGGATTGAGGAGGAGTGCCACCGCGTGGAGGCCCAGCTGGCCCTGAAGGAGGCCCGAAAG
  GAGATCAAGCAGCTCAAGCAGGTCATCGACACTGTCAAGAACAACCTGATTGACAAGGACAAGGGGCTGC
  AGAAGTACTTCGTGGACATCAACATCCAGAACAAGAAGCTGGAGACGCTGCTGCACAGCATGGAGGTGGC
  CCAGAATGGCATGGCCAAGGAGGATGGCACTGGGGAGTCAGCCGGTGGGTCCCCTGCCCGCTCCCTCACC
  CGCAGCTCCACCTACACCAAGCTGAGTGACCCGGCTGTCTGTGGTGACCGCCAGCCGGGTGATCCCTCCA
  GCGGCTCTGCTGAGGATGGGGCAGACAGTGGCTTTGCAGCAGCCGATGACACACTGAGCCGGACGGACGC
  GCTGGAAGCCAGCAGCCTGCTGTCGTCGGGGGTGGACTGTGGCACCGAGGAGACCTCGCTGCACAGCTCC
  TTCGGCCTGGGCCCCCGCTTCCCTGCCAGCAACACCTATGAGAAGCTGCTGTGTGGCATGGAGGCTGGTG
  TGCAGGCCAGCTGCATGCAGGAGCGTGCCATCCAGACAGACTTCGTGCAGTACCAGCCTGACCTTGACAC
  CATCCTGGAGAAAGTGACCCAGGCCCAGGTCTGTGGGACAGACCCTGAGTCAGGGGACAGGTGCCCAGAG
  CTGGATGCCCACCCTTCAGGGCCCAGAGACCCCAACTCAGCAGTGGTGGTGACAGTGGGTGATGAGCTAG
  AGGCCCCAGAGCCCATCACCCGTGGACCCACCCCACAGCGGCCTGGTGCCAACCCCAACCCTGGCCAGTC
  GGTGAGCGTGGTGTGCCCCATGGAAGAGGAGGAGGAGGCTGCCGTGGCTGAGAAGGAGCCCAAGAGCTAC
  TGGAGCCGCCACTACATCGTGGATCTGCTGGCTGTGGTGGTGCCGGCCGTGCCCACGGTGGCCTGGCTTT
  GCCGCTCCCAGCGGCGCCAGGGCCAGCCCATCTACAACATCAGCTCCCTGCTGCGGGGCTGCTGCACTGT
  GGCCTTGCACTCCATCCGCAGGATCAGCTGCCGCTCGCTGAGCCAGCCGAGTCCCAGCCCAGCGGGCGGC
  GGCTCCCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207749 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMSLPGSRRTSAGSRRRTSPPVSVRDAYGTSSLSSSSNSGSYKGSDSSPTPRRSMKYTLCSDNHGIKPP
  TPEQYLTPLQQKEVCIRHLKARLKDTQDRLQDRDTEIDDLKTQLSRMQEDWIEEECHRVEAQLALKEARK
  EIKQLKQVIDTVKNNLIDKDKGLQKYFVDINIQNKKLETLLHSMEVAQNGMAKEDGTGESAGGSPARSLT
  RSSTYTKLSDPAVCGDRQPGDPSSGSAEDGADSGFAAADDTLSRTDALEASSLLSSGVDCGTEETSLHSS
  FGLGPRFPASNTYEKLLCGMEAGVQASCMQERAIQTDFVQYQPDLDTILEKVTQAQVCGTDPESGDRCPE
  LDAHPSGPRDPNSAVVVTVGDELEAPEPITRGPTPQRPGANPNPGQSVSVVCPMEEEEEAAVAEKEPKSY
  WSRHYIVDLLAVVVPAVPTVAWLCRSQRRQGQPIYNISSLLRGCCTVALHSIRRISCRSLSQPSPSPAGG
  GSQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014723
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014723.4
• RefSeq Size: 5026 bp
• RefSeq ORF: 1485 bp
• Locus ID: 9751
• UniProt ID: O15079
• Cytogenetics: 20p13
• MW: 53.5 kDa
• Gene Summary: Syntaxin-1, synaptobrevin/VAMP, and SNAP25 interact to form the SNARE complex, which is required for synaptic vesicle docking and fusion. The protein encoded by this gene is membrane-associated and inhibits SNARE complex formation by binding free syntaxin-1. Expression of this gene appears to be brain-specific. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]