ETV7 (NM_016135) Human Tagged ORF Clone

CAT#: RC207742

ETV7 (Myc-DDK-tagged)-Human ets variant 7 (ETV7), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ETV7 (Cat# RC207742, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETV7(Cat# TA803463). Positive lysates LY414164 (100ug) and LC414164 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414164) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207742 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ETV7 protein (Cat# TP307742). The protein was produced from HEK293T cells transfected with ETV7 cDNA clone (Cat# RC207742) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETV7
• Synonyms: TEL-2; TEL2; TELB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207742 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGAGGGAGAATTGGCTATTTCTCCTATAAGCCCTGTGGCAGCCATGCCTCCCCTAGGCACCCACG
  TGCAAGCCAGATGTGAAGCTCAAATTAACCTGCTGGGTGAAGGGGGGATCTGCAAGCTGCCAGGAAGACT
  CCGCATCCAGCCCGCACTGTGGAGCAGGGAGGACGTGCTGCACTGGCTGCGCTGGGCAGAGCAGGAGTAC
  TCTCTGCCATGCACCGCGGAGCACGGGTTCGAGATGAACGGACGCGCCCTCTGCATCCTCACCAAGGACG
  ACTTCCGGCACCGTGCGCCCAGCTCAGGTGACGTCCTGTATGAGCTGCTCCAGTACATCAAGACCCAGCG
  GCGAGCCCTGGTGTGTGGACCCTTTTTTGGAGGGATCTTCAGGCTGAAGACGCCCACCCAGCACTCTCCA
  GTCCCCCCGGAAGAGGTGACTGGCCCCTCTCAGATGGACACCCGAAGGGGCCACCTGCTGCAGCCACCAG
  ACCCAGGGCTTACCAGCAACTTCGGCCACCTGGATGACCCTGGCCTGGCAAGGTGGACCCCTGGCAAGGA
  GGAGTCCCTCAACTTATGTCACTGTGCAGAGCTCGGCTGCAGGACCCAGGGGGTCTGTTCCTTCCCCGCG
  ATGCCGCAGGCCCCCATTGACGGCAGGATCGCTGACTGCCGCCTGCTGTGGGATTACGTGTATCAGCTGC
  TCCTTGATACCCGATATGAGCCCTACATCAAGTGGGAAGACAAGGACGCCAAGATCTTCCGAGTTGTGGA
  TCCAAATGGGCTCGCCAGACTCTGGGGAAATCACAAGAACCGGGTGAACATGACCTACGAGAAGATGTCT
  CGTGCCCTGCGCCACTATTATAAGCTTAATATCATTAAGAAGGAACCGGGGCAGAAACTCCTGTTCAGAT
  TTCTAAAGACTCCGGGAAAGATGGTCCAGGACAAGCACAGCCACCTGGAGCCGCTGGAGAGCCAGGAGCA
  GGACAGAATAGAGTTCAAGGACAAGAGGCCAGAAATCTCTCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207742 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQEGELAISPISPVAAMPPLGTHVQARCEAQINLLGEGGICKLPGRLRIQPALWSREDVLHWLRWAEQEY
  SLPCTAEHGFEMNGRALCILTKDDFRHRAPSSGDVLYELLQYIKTQRRALVCGPFFGGIFRLKTPTQHSP
  VPPEEVTGPSQMDTRRGHLLQPPDPGLTSNFGHLDDPGLARWTPGKEESLNLCHCAELGCRTQGVCSFPA
  MPQAPIDGRIADCRLLWDYVYQLLLDTRYEPYIKWEDKDAKIFRVVDPNGLARLWGNHKNRVNMTYEKMS
  RALRHYYKLNIIKKEPGQKLLFRFLKTPGKMVQDKHSHLEPLESQEQDRIEFKDKRPEISP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016135
• ORF Size: 1023 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016135.2, NP_057219.1
• RefSeq Size: 1761 bp
• RefSeq ORF: 1026 bp
• Locus ID: 51513
• UniProt ID: Q9Y603
• Cytogenetics: 6p21.31
• Domains: ETS, SAM_PNT, SAPA, SapB_1, SapB_2, SAPB
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Dorso-ventral axis formation
• MW: 39 kDa
• Gene Summary: The protein encoded by this gene belongs to the ETS family of transcription factors, which is a large group of evolutionarily conserved transcriptional regulators that play an important role in a variety of cellular processes throughout development and differentiation, and are involved in oncogenesis as well. This protein is predominantly expressed in hematopoietic tissues. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene (PMID:11108721).[provided by RefSeq, May 2011]