KIF2B (NM_032559) Human Tagged ORF Clone

CAT#: RC207433

KIF2B (Myc-DDK-tagged)-Human kinesin family member 2B (KIF2B)

Product Images

Circular map for RC207433

• 

  Western blot validation of overexpression lysate (Cat# LY410047) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207433 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207433 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCCAGTTCTGCCTCCCTGAATCCCCATGTCTCTCGCCCCTGAAACCCTTGAAGCCACATTTCG
  GAGACATCCAAGAGGGCATCTACGTGGCGATCCAGCGCAGTGACAAGCGGATCCACCTCGCTGTGGTCAC
  GGAGATCAACAGAGAAAACTATTGGGTCACGGTAGAGTGGGTGGAGAAAGCAGTCAAAAAAGGCAAGAAG
  ATTGACCTGGAGACCATACTCCTGCTGAATCCAGCTCTGGACTCTGCTGAACACCCCATGCCGCCCCCGC
  CCTTATCCCCCTTGGCTCTGGCGCCCTCTTCGGCCATCAGGGACCAGCGTACCGCCACGAAATGGGTTGC
  GATGATCCCCCAGAAAAACCAAACAGCCTCAGGGGACAGCCTGGATGTGAGGGTCCCCAGCAAACCTTGT
  CTGATGAAGCAGAAAAAGTCTCCCTGCCTCTGGGAAATCCAGAAACTGCAGGAGCAGCGGGAAAAGCGCA
  GGCGGCTGCAGCAGGAGATCCGAGCTAGACGCGCCCTCGATGTCAATACCAGAAACCCCAACTACGAAAT
  CATGCACATGATCGAAGAGTATCGCAGGCACCTGGACAGCAGCAAGATCTCAGTCCTGGAGCCCCCGCAA
  GAACATCGCATCTGCGTCTGCGTGAGGAAGCGGCCTCTCAACCAGCGAGAGACAACCTTGAAGGACCTGG
  ATATCATCACCGTCCCCTCGGACAATGTGGTTATGGTGCATGAGTCCAAGCAAAAGGTGGACCTCACTCG
  CTACCTGCAGAACCAGACCTTCTGCTTCGACCATGCCTTCGATGACAAAGCCTCCAACGAGTTGGTGTAC
  CAGTTCACCGCCCAGCCACTGGTGGAGTCCATCTTCCGCAAGGGCATGGCCACCTGCTTTGCCTATGGGC
  AGACGGGAAGTGGGAAGACGTACACCATGGGTGGAGACTTTTCAGGAACGGCCCAAGATTGTTCTAAGGG
  CATTTATGCTTTGGTGGCACAGGATGTCTTTCTCCTGCTCAGAAACTCCACATATGAGAAGCTGGACCTC
  AAAGTCTATGGGACATTTTTTGAGATTTATGGGGGCAAGGTGTATGATTTGTTGAACTGGAAGAAGAAGC
  TGCAAGTCCTTGAGGATGGCAATCAGCAAATCCAAGTGGTCGGGCTGCAGGAGAAAGAGGTGTGTTGTGT
  GGAGGAAGTGCTGAACCTGGTGGAAATAGGGAATAGCTGTCGGACTTCCAGGCAAACATCTGTCAACGCT
  CACTCATCCAGGAGCCATGCAGTGTTCCAGATCATCCTGAAGTCAGGAGGGATAATGCATGGCAAGTTTT
  CCCTCGTTGATTTAGCTGGGAATGAAAGAGGAGCAGATACAACCAAGGCCAGCCGGAAAAGGCAGCTGGA
  AGGGGCAGAGATTAACAAGAGTCTTCTAGCCCTCAAAGAATGTATTCTGGCTTTGGGTCAGAACAAGCCT
  CACACCCCATTCAGAGCCAGCAAACTCACACTGGTGCTCCGGGACTCCTTTATAGGCCAGAACTCCTCCA
  CTTGCATGATTGCTACCATCTCTCCGGGGATGACCTCTTGTGAAAACACTCTCAACACTTTAAGATATGC
  AAACAGAGTAAAAAAATTAAATGTAGATGTAAGGCCCTACCATCGTGGCCACTATCCGATTGGACATGAG
  GCACCAAGGATGTTAAAAAGTCACATCGGAAATTCAGAAATGTCCCTTCAGAGGGATGAATTTATTAAAA
  TACCTTATGTACAGAGTGAGGAGCAGAAAGAGATTGAAGAGGTTGAAACATTACCCACTCTGTTAGGGAA
  GGATACCACAATTTCAGGGAAGGGATCTAGCCAATGGTTGGAAAACATCCAGGAGAGAGCTGGTGGAGTA
  CACCATGATATTGATTTTTGCATTGCCCGGTCTTTGTCCATTTTGGAGCAGAAAATTGATGCTCTGACCG
  AGATCCAAAAGAAACTGAAATTATTACTAGCTGACCTCCACGTGAAGAGCAAGGTAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207433 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASQFCLPESPCLSPLKPLKPHFGDIQEGIYVAIQRSDKRIHLAVVTEINRENYWVTVEWVEKAVKKGKK
  IDLETILLLNPALDSAEHPMPPPPLSPLALAPSSAIRDQRTATKWVAMIPQKNQTASGDSLDVRVPSKPC
  LMKQKKSPCLWEIQKLQEQREKRRRLQQEIRARRALDVNTRNPNYEIMHMIEEYRRHLDSSKISVLEPPQ
  EHRICVCVRKRPLNQRETTLKDLDIITVPSDNVVMVHESKQKVDLTRYLQNQTFCFDHAFDDKASNELVY
  QFTAQPLVESIFRKGMATCFAYGQTGSGKTYTMGGDFSGTAQDCSKGIYALVAQDVFLLLRNSTYEKLDL
  KVYGTFFEIYGGKVYDLLNWKKKLQVLEDGNQQIQVVGLQEKEVCCVEEVLNLVEIGNSCRTSRQTSVNA
  HSSRSHAVFQIILKSGGIMHGKFSLVDLAGNERGADTTKASRKRQLEGAEINKSLLALKECILALGQNKP
  HTPFRASKLTLVLRDSFIGQNSSTCMIATISPGMTSCENTLNTLRYANRVKKLNVDVRPYHRGHYPIGHE
  APRMLKSHIGNSEMSLQRDEFIKIPYVQSEEQKEIEEVETLPTLLGKDTTISGKGSSQWLENIQERAGGV
  HHDIDFCIARSLSILEQKIDALTEIQKKLKLLLADLHVKSKVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032559
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032559.5
• RefSeq Size: 2335 bp
• RefSeq ORF: 2022 bp
• Locus ID: 84643
• UniProt ID: Q8N4N8
• Cytogenetics: 17q22
• Protein Families: Druggable Genome
• MW: 76.1 kDa
• Gene Summary: Plus end-directed microtubule-dependent motor required for spindle assembly and chromosome movement. Has microtubule depolymerization activity (PubMed:17538014). Plays a role in chromosome congression (PubMed:23891108).[UniProtKB/Swiss-Prot Function]