KCND1 (NM_004979) Human Tagged ORF Clone

CAT#: RC207353

KCND1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 1 (KCND1)

Product Images

Circular map for RC207353

• 

  Western blot validation of overexpression lysate (Cat# LY401548) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207353 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCND1
• Synonyms: KV4.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207353 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGGCCTGGCCACGTGGCTGCCTTTTGCTCGGGCAGCAGCAGTGGGCTGGCTGCCCCTGGCCC
  AGCAACCCCTGCCCCCGGCACCGGGGGTGAAGGCATCTCGAGGAGATGAGGTTCTGGTGGTGAACGTGAG
  CGGACGGCGCTTTGAGACTTGGAAGAATACGCTGGACCGCTACCCAGACACCTTGCTGGGCAGCTCGGAG
  AAGGAATTCTTCTACGATGCTGACTCAGGCGAGTACTTCTTCGATCGCGACCCTGACATGTTCCGCCATG
  TGCTGAACTTCTACCGAACGGGGCGGCTGCATTGCCCACGGCAGGAGTGCATCCAGGCCTTCGACGAAGA
  GCTGGCTTTCTACGGCCTGGTTCCCGAGCTAGTCGGTGACTGCTGCCTTGAAGAGTATCGGGACCGAAAG
  AAGGAGAATGCCGAGCGCCTGGCAGAGGATGAGGAGGCAGAGCAGGCCGGGGACGGCCCAGCCCTGCCAG
  CAGGCAGCTCCCTGCGGCAGCGGCTCTGGCGGGCCTTCGAGAATCCACACACGAGCACCGCAGCCCTCGT
  TTTCTACTATGTGACCGGCTTCTTCATCGCCGTGTCGGTCATCGCCAATGTGGTGGAGACCATCCCATGC
  CGCGGCTCTGCACGCAGGTCCTCAAGGGAGCAGCCCTGTGGCGAACGCTTCCCACAGGCCTTTTTCTGCA
  TGGACACAGCCTGTGTACTCATATTCACAGGTGAATACCTCCTGCGGCTGTTTGCCGCCCCCAGCCGTTG
  CCGCTTCCTGCGGAGTGTCATGAGCCTCATCGACGTGGTGGCCATCCTGCCCTACTACATTGGGCTTTTG
  GTGCCCAAGAACGACGATGTCTCTGGCGCCTTTGTCACCCTGCGTGTGTTCCGGGTGTTTCGCATCTTCA
  AGTTCTCCAGGCACTCACAGGGCTTGAGGATTCTGGGCTACACACTCAAGAGCTGTGCCTCTGAGCTGGG
  CTTTCTCCTCTTTTCCCTAACCATGGCCATCATCATCTTTGCCACTGTCATGTTTTATGCTGAGAAGGGC
  ACAAACAAGACCAACTTTACAAGCATCCCTGCGGCCTTCTGGTATACCATTGTCACCATGACCACGCTTG
  GCTACGGAGACATGGTGCCCAGCACCATTGCTGGCAAGATTTTCGGGTCCATCTGCTCACTCAGTGGCGT
  CTTGGTCATTGCCCTGCCTGTGCCAGTCATTGTGTCCAACTTTAGCCGCATCTACCACCAGAACCAGCGG
  GCTGACAAGCGCCGAGCACAGCAGAAGGTGCGCTTGGCAAGGATCCGATTGGCAAAGAGTGGTACCACCA
  ATGCCTTCCTGCAGTACAAGCAGAATGGGGGCCTTGAGGACAGCGGCAGTGGCGAGGAACAGGCTCTTTG
  TGTCAGGAACCGTTCTGCCTTTGAACAGCAACATCACCACTTGCTGCACTGTCTAGAGAAGACAACGTGC
  CATGAGTTCACAGATGAGCTCACCTTCAGTGAAGCCCTGGGAGCCGTCTCGCCGGGTGGCCGCACCAGCC
  GTAGCACCTCTGTGTCTTCCCAGCCAGTGGGACCCGGAAGCCTGCTGTCTTCTTGCTGCCCTCGCAGGGC
  CAAGCGCCGCGCCATCCGCCTTGCCAACTCCACTGCCTCAGTCAGCCGTGGCAGCATGCAGGAGCTGGAC
  ATGCTGGCAGGGCTGCGCAGGAGCCATGCCCCTCAGAGCCGCTCCAGCCTCAATGCCAAGCCCCATGACA
  GCCTTGACCTGAACTGCGACAGCCGGGACTTCGTGGCTGCCATTATCAGCATCCCTACCCCTCCTGCCAA
  CACCCCAGATGAGAGCCAACCTTCCTCCCCTGGCGGCGGTGGCAGGGCCGGCAGCACCCTCAGGAACTCC
  AGCCTGGGTACCCCTTGCCTCTTCCCCGAGACTGTCAAGATCTCATCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207353 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGLATWLPFARAAAVGWLPLAQQPLPPAPGVKASRGDEVLVVNVSGRRFETWKNTLDRYPDTLLGSSE
  KEFFYDADSGEYFFDRDPDMFRHVLNFYRTGRLHCPRQECIQAFDEELAFYGLVPELVGDCCLEEYRDRK
  KENAERLAEDEEAEQAGDGPALPAGSSLRQRLWRAFENPHTSTAALVFYYVTGFFIAVSVIANVVETIPC
  RGSARRSSREQPCGERFPQAFFCMDTACVLIFTGEYLLRLFAAPSRCRFLRSVMSLIDVVAILPYYIGLL
  VPKNDDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKG
  TNKTNFTSIPAAFWYTIVTMTTLGYGDMVPSTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQR
  ADKRRAQQKVRLARIRLAKSGTTNAFLQYKQNGGLEDSGSGEEQALCVRNRSAFEQQHHHLLHCLEKTTC
  HEFTDELTFSEALGAVSPGGRTSRSTSVSSQPVGPGSLLSSCCPRRAKRRAIRLANSTASVSRGSMQELD
  MLAGLRRSHAPQSRSSLNAKPHDSLDLNCDSRDFVAAIISIPTPPANTPDESQPSSPGGGGRAGSTLRNS
  SLGTPCLFPETVKISSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004979
• ORF Size: 1941 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_004979.4, NP_004970.3
• RefSeq Size: 4720 bp
• RefSeq ORF: 1944 bp
• Locus ID: 3750
• UniProt ID: Q9NSA2
• Cytogenetics: Xp11.23
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 71.3 kDa
• Gene Summary: This gene encodes a multipass membrane protein that comprises the pore subunit of the voltage-gated A-type potassium channel, which functions in the repolarization of membrane action potentials. Activity of voltage-gated potassium channels is important in a number of physiological processes, among them the regulation of neurotransmitter release, heart rate, insulin secretion, and smooth muscle contraction. [provided by RefSeq, Aug 2013]