Choline kinase alpha(CHKA) (NM_212469) Human Tagged ORF Clone

CAT#: RC207209

CHKA (Myc-DDK-tagged)-Human choline kinase alpha (CHKA), transcript variant 2

Clone Modification / Custom Cloning Service

USD 577.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name Choline kinase alpha(CHKA) (NM_212469) Human Tagged ORF Clone
Symbol CHKA
Synonyms CHK; CK; CKI; EK
Vector pCMV6-Entry
Sequence Data
>RC207209 representing NM_212469
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCCGCTCGGGCTGCTGCTGAGCTGCGGTA
GCGGCAGCGCGGCCCCGGCGCCCGGCGTGGGGCAGCAGCGCGACGCCGCCAGCGACCTCGAGTCCAAGCA
GCTGGGCGGCCAACAGCCGCCGCTCGCGCTGCCCCCTCCGCCGCCGCTGCCGCTGCCGCTGCCGCTGCCC
CAGCCCCCGCCGCCGCAGCCGCCCGCAGACGAGCAGCCGGAGCCCCGGACGCGGCGCAGGGCCTATCTGT
GGTGCAAGGAGTTCCTGCCCGGCGCCTGGCGGGGCCTCCGCGAGGACGAGTTCCACATCAGTGTCATCAG
AGGCGGCCTTAGCAACATGCTGTTCCAGTGCTCCCTACCTGACACCACAGCCACCCTTGGTGATGAGCCT
CGGAAAGTGCTCCTGCGGCTGTATGGAGCGATTTTGCAGGTGGGGGCTGAGGCCATGGTTCTGGAGAGCG
TTATGTTTGCCATTCTCGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTTCCCCAAGGCCGACT
GGAGCAGTTCATCCCGAGCCGGCGATTAGATACTGAAGAATTAGGTTTGCCAGATATTTCTGCAGAAATC
GCCGAGAAAATGGCTACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAATGGCTTTTTGGCA
CAATGGAAAAGTATCTAAAGGAAGTGCTGAGAATTAAATTTACTGAGGAATCCAGAATTAAAAAGCTCCA
CAAATTGCTCAGTTACAATCTGCCCTTGGAACTGGAAAACCTGAGATCATTGCTTGAATCTACTCCATCT
CCAGTTGTATTTTGTCATAATGACTGTCAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAATTCTG
AAAAACAGAAACTGATGCTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGATTCGACATTGGAAA
TCACTTCTGTGAGTGGATGTATGATTATAGCTATGAAAAATACCCTTTTTTCAGAGCAAACATCCGGAAG
TATCCCACCAAGAAACAACAGCTCCATTTTATTTCCAGTTACTTGCCTGCATTCCAAAATGACTTTGAAA
ACCTCAGTACTGAAGAAAAATCCATTATAAAAGAAGAAATGTTGCTTGAAGTTAATAGGTTTGCCCTTGC
ATCTCATTTCCTCTGGGGACAGTGGTCCATTGTACAAGCCAAGATTTCATCTATTGAATTTGGGTACATG
GACTACGCCCAAGCAAGGTTTGATGCCTATTTCCACCAGAAGAGGAAGCTTGGGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207209 representing NM_212469
Red=Cloning site Green=Tags(s)

MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPPPPLPLPLPLP
QPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEP
RKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEI
AEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPS
PVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRK
YPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYM
DYAQARFDAYFHQKRKLGV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_212469
ORF Size 1317 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_212469.1, NP_997634
RefSeq Size 2679
RefSeq ORF 1320
Locus ID 1119
Cytogenetics 11q13.2
Protein Families Druggable Genome
Protein Pathways Glycerophospholipid metabolism, Metabolic pathways
MW 50 kDa
Gene Summary The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
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Citations (1)