LMAN1 (NM_005570) Human Tagged ORF Clone

CAT#: RC207088

LMAN1 (Myc-DDK-tagged)-Human lectin, mannose-binding, 1 (LMAN1)

Product Images

Circular map for RC207088

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  Western blot validation of overexpression lysate (Cat# LY401709) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207088 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LMAN1 protein (Cat# TP307088). The protein was produced from HEK293T cells transfected with LMAN1 cDNA clone (Cat# RC207088) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LMAN1 (Cat# RC207088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1(Cat# TA502137). Positive lysates LY401709 (100ug) and LC401709 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LMAN1
• Synonyms: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; MCFD1; MR60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207088 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGATCCAGGCAAAGGGGTCTCCGGGCCAGAGTTCGGCCGCTGTTCTGCGCCTTGCTGCTGTCAC
  TCGGTCGCTTCGTCCGGGGCGACGGCGTGGGAGGAGACCCCGCGGTCGCGTTGCCACATCGCCGTTTCGA
  GTACAAATACAGCTTCAAGGGGCCGCACCTGGTGCAGAGCGACGGGACCGTGCCCTTCTGGGCCCACGCG
  GGGAATGCTATTCCAAGTTCAGATCAAATTCGAGTAGCACCATCTTTAAAAAGCCAAAGAGGCTCAGTGT
  GGACAAAGACAAAAGCGGCCTTTGAGAACTGGGAAGTTGAGGTGACATTTCGAGTGACTGGAAGAGGTCG
  AATTGGAGCTGATGGCCTAGCAATTTGGTATGCAGAAAATCAAGGCTTGGAGGGCCCTGTGTTTGGATCA
  GCTGATCTGTGGAATGGTGTTGGAATATTTTTTGATTCTTTTGACAATGATGGAAAGAAAAATAATCCTG
  CTATAGTAATTATAGGCAACAATGGACAAATCCATTATGACCATCAAAATGACGGGGCTAGTCAAGCTTT
  GGCAAGTTGCCAGAGGGACTTCCGCAACAAACCCTATCCTGTCCGAGCAAAGATTACCTATTACCAGAAC
  ACACTGACAGTAATGATCAATAATGGCTTTACACCAGATAAAAATGATTATGAATTTTGTGCCAAAGTGG
  AAAATATGATTATCCCTGCACAAGGGCATTTTGGAATATCTGCTGCAACTGGAGGTCTTGCAGATGACCA
  TGATGTCCTTTCTTTTCTGACTTTCCAGTTGACTGAACCTGGAAAAGAGCCGCCCACACCAGATAAAGAA
  ATTTCGGAAAAGGAAAAAGAAAAGTATCAGGAGGAATTTGAGCACTTTCAACAAGAATTGGATAAAAAAA
  AAGAGGAATTCCAGAAGGGCCACCCCGACCTCCAAGGGCAGCCTGCGGAGGAAATATTTGAGAGTGTAGG
  AGATCGAGAGCTAAGACAAGTCTTTGAAGGACAGAATCGTATTCATCTTGAAATCAAGCAGCTGAACCGG
  CAGTTAGATATGATTCTTGATGAACAGAGAAGATATGTCTCTTCCTTAACAGAGGAAATCTCTAAAAGAG
  GAGCAGGAATGCCTGGGCAGCATGGGCAGATTACTCAACAAGAACTGGATACTGTTGTGAAAACTCAGCA
  TGAGATTCTGAGACAAGTAAATGAAATGAAAAATTCCTTGAGTGAAACCGTCAGACTGGTCAGTGGAATG
  CAGCACCCTGGCTCTGCTGGAGGCGTCTATGAGACAACACAGCACTTCATTGACATCAAAGAGCACCTGC
  ACATAGTAAAGAGGGACATAGATAACTTAGTGCAGCGAAATATGCCATCAAATGAAAAGCCGAAATGCCC
  AGAACTACCACCATTTCCATCATGTTTGTCTACGGTCCACTTCATTATATTTGTTGTGGTGCAAACTGTA
  TTATTCATTGGTTATATCATGTATAGGTCTCAGCAAGAAGCAGCTGCCAAAAAATTCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207088 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGSRQRGLRARVRPLFCALLLSLGRFVRGDGVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHA
  GNAIPSSDQIRVAPSLKSQRGSVWTKTKAAFENWEVEVTFRVTGRGRIGADGLAIWYAENQGLEGPVFGS
  ADLWNGVGIFFDSFDNDGKKNNPAIVIIGNNGQIHYDHQNDGASQALASCQRDFRNKPYPVRAKITYYQN
  TLTVMINNGFTPDKNDYEFCAKVENMIIPAQGHFGISAATGGLADDHDVLSFLTFQLTEPGKEPPTPDKE
  ISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNR
  QLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSLSETVRLVSGM
  QHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLSTVHFIIFVVVQTV
  LFIGYIMYRSQQEAAAKKFF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005570
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005570.4
• RefSeq Size: 4848 bp
• RefSeq ORF: 1533 bp
• Locus ID: 3998
• UniProt ID: P49257
• Cytogenetics: 18q21.32
• Domains: Lectin_leg-like
• Protein Families: Druggable Genome, Transmembrane
• MW: 57.5 kDa
• Gene Summary: The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015]