LMAN1 (NM_005570) Human Untagged Clone

CAT#: SC125316

LMAN1 (untagged)-Human lectin, mannose-binding, 1 (LMAN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LMAN1
• Synonyms: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; MCFD1; MR60
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125316 sequence for NM_005570 edited (data generated by NextGen Sequencing)
  ATGGCGGGATCCAGGCAAAGGGGTCTCCGGGCCAGAGTTCGGCCGCTGTTCTGCGCCTTG
  CTGCTGTCACTCGGTCGCTTCGTCCGGGGCGACGGCGTGGGAGGAGACCCCGCGGTCGCG
  TTGCCACATCGCCGTTTCGAGTACAAATACAGCTTCAAGGGGCCGCACCTGGTGCAGAGC
  GACGGGACCGTGCCCTTCTGGGCCCACGCGGGGAATGCTATTCCAAGTTCAGATCAAATT
  CGAGTAGCACCATCTTTAAAAAGCCAAAGAGGCTCAGTGTGGACAAAGACAAAAGCGGCC
  TTTGAGAACTGGGAAGTTGAGGTGACATTTCGAGTGACTGGAAGAGGTCGAATTGGAGCT
  GATGGCCTAGCAATTTGGTATGCAGAAAATCAAGGCTTGGAGGGCCCTGTGTTTGGATCA
  GCTGATCTGTGGAATGGTGTTGGAATATTTTTTGATTCTTTTGACAATGATGGAAAGAAA
  AATAATCCTGCTATAGTAATTATAGGCAACAATGGACAAATCCATTATGACCATCAAAAT
  GACGGGGCTAGTCAAGCTTTGGCAAGTTGCCAGAGGGACTTCCGCAACAAACCCTATCCT
  GTCCGAGCAAAGATTACCTATTACCAGAACACACTGACAGTAATGATCAATAATGGCTTT
  ACACCAGATAAAAATGATTATGAATTTTGTGCCAAAGTGGAAAATATGATTATCCCTGCA
  CAAGGGCATTTTGGAATATCTGCTGCAACTGGAGGTCTTGCAGATGACCATGATGTCCTT
  TCTTTTCTGACTTTCCAGTTGACTGAACCTGGAAAAGAGCCGCCCACACCAGATAAAGAA
  ATTTCGGAAAAGGAAAAAGAAAAGTATCAGGAGGAATTTGAGCACTTTCAACAAGAATTG
  GATAAAAAAAAAGAGGAATTCCAGAAGGGCCACCCCGACCTCCAAGGGCAGCCTGCGGAG
  GAAATATTTGAGAGTGTAGGAGATCGAGAGCTAAGACAAGTCTTTGAAGGACAGAATCGT
  ATTCATCTTGAAATCAAGCAGCTGAACCGGCAGTTAGATATGATTCTTGATGAACAGAGA
  AGATATGTCTCTTCCTTAACAGAGGAAATCTCTAAAAGAGGAGCAGGAATGCCTGGGCAG
  CATGGGCAGATTACTCAACAAGAACTGGATACTGTTGTGAAAACTCAGCATGAGATTCTG
  AGACAAGTAAATGAAATGAAAAATTCCATGAGTGAAACCGTCAGACTGGTCAGTGGAATG
  CAGCACCCTGGCTCTGCTGGAGGCGTCTATGAGACAACACAGCACTTCATTGACATCAAA
  GAGCACCTGCACATAGTAAAGAGGGACATAGATAACTTAGTGCAGCGAAATATGCCATCA
  AATGAAAAGCCGAAATGCCCAGAACTACCACCATTTCCATCATGTTTGTCTACGGTCCAC
  TTCATTATATTTGTTGTGGTGCAAACTGTATTATTCATTGGTTATATCATGTATAGGTCT
  CAGCAAGAAGCAGCTGCCAAAAAATTCTTTTGA
  
  Clone variation with respect to NM_005570.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005570 unedited
  TGATATCCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA
  AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC
  GCGAATTCGGCACGAGGATGGCGGGATCCAGGCAAAGGGGTCTCCGGGCCAGAGTTCGGC
  CGCTGTTCTGCGCCTTGCTGCTGTCACTCGGTCGCTTCGTCCGGGGCGACGGCGTGGGAG
  GAGACCCCGCGGTCGCGTTGCCACATCGCCGTTTCGAGTACAAATACAGCTTCAAGGGGC
  CGCACCTGGTGCAGAGCGACGGGACCGTGCCCTTCTGGGCCCACGCGGGGAATGCTATTC
  CAAGTTCAGATCAAATTCGAGTAGCACCATCTTTAAAAAGCCAAAGAGGCTCAGTGTGGA
  CAAAGACAAAAGCGGCCTTTGAGAACTGGGAAGTTGAGGTGACATTTCGAGTGACTGGAA
  GAGGTCGAATTGGAGCTGATGGCCTAGCAATTTGGTATGCAGAAAATCAAGGCTTGGAGG
  GCCCTGTGTTTGGATCAGCTGATCTGTGGAATGGTGTTGGAATATTTTTTGATTCTTTTG
  ACAATGATGGAAAGAAAAATAATCCTGCTATAGTAATTATAGGCAACAATGGACAAATCC
  ATTATGACCATCAAAATGACGGGGCTAGTCAAGCTTTGGCAAGNNTGCCAGAGGGACTTT
  CCGCACAAACCCTATCCTGTNCGAGCAAAGATACCTATTACCGAACACACTTGACAGTAT
  GATCAATAATGCTTTACACCANATAAAAATGATTATTGATTTTTGTGCCCAAGTGGGAAA
  ATATGATATTCCTGCACAGGGGCATTTTGGATATCTGCTGCACTGGAGGTCTTGCAGATG
  ACCATGAGCCTTNCTTTCTGACTTTCAGTTGACTGAACTGGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005570
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005570.2, NP_005561.1
• RefSeq Size: 2764 bp
• RefSeq ORF: 1533 bp
• Locus ID: 3998
• UniProt ID: P49257
• Cytogenetics: 18q21.32
• Domains: Lectin_leg-like
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015]