B3GNT4 (NM_030765) Human Tagged ORF Clone

CAT#: RC206795

B3GNT4 (Myc-DDK-tagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4 (B3GNT4)

Product Images

Circular map for RC206795

• 

  Western blot validation of overexpression lysate (Cat# LY410727) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206795 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B3GNT4
• Synonyms: B3GN-T4; beta3Gn-T4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206795 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCTCCCCAGGCTTCCGCAGCCCACCAGGGAAGGGGCGGTAGGAGTGGCCTTTTACCAAAGGGAC
  CGGCGATGCTCTGCAGGCTGTGCTGGCTGGTCTCGTACAGCTTGGCTGTGCTGTTGCTCGGCTGCCTGCT
  CTTCCTGAGGAAGGCGGCCAAGCCCGCAGGAGACCCCACGGCCCACCAGCCTTTCTGGGCTCCCCCAACA
  CCCCGTCACAGCCGGTGTCCACCCAACCACACAGTGTCTAGCGCCTCTCTGTCCCTGCCTAGCCGTCACC
  GTCTCTTCTTGACCTATCGTCACTGCCGAAATTTCTCTATCTTGCTGGAGCCTTCAGGCTGTTCCAAGGA
  TACCTTCTTGCTCCTGGCCATCAAGTCACAGCCTGGTCACGTGGAGCGACGTGCGGCTATCCGCAGCACG
  TGGGGCAGGGTGGGGGGATGGGCTAGGGGCCGGCAGCTGAAGCTGGTGTTCCTCCTAGGGGTGGCAGGAT
  CCGCTCCCCCAGCCCAGCTGCTGGCCTATGAGAGTAGGGAGTTTGATGACATCCTCCAGTGGGACTTCAC
  TGAGGACTTCTTCAACCTGACGCTCAAGGAGCTGCACCTGCAGCGCTGGGTGGTGGCTGCCTGCCCCCAG
  GCCCATTTCATGCTAAAGGGAGATGACGATGTCTTTGTCCACGTCCCCAACGTGTTAGAGTTCCTGGATG
  GCTGGGACCCAGCCCAGGACCTCCTGGTGGGAGATGTCATCCGCCAAGCCCTGCCCAACAGGAACACTAA
  GGTCAAATACTTCATCCCACCCTCAATGTACAGGGCCACCCACTACCCACCCTATGCTGGTGGGGGAGGA
  TATGTCATGTCCAGAGCCACAGTGCGGCGCCTCCAGGCTATCATGGAAGATGCTGAACTCTTCCCCATTG
  ATGATGTCTTTGTGGGTATGTGCCTGAGGAGGCTGGGGCTGAGCCCTATGCACCATGCTGGCTTCAAGAC
  ATTTGGAATCCGGCGGCCCCTGGACCCCTTAGACCCCTGCCTGTATAGGGGGCTCCTGCTGGTTCACCGC
  CTCAGCCCCCTCGAGATGTGGACCATGTGGGCACTGGTGACAGATGAGGGGCTCAAGTGTGCAGCTGGCC
  CCATACCCCAGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206795 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPPQASAAHQGRGGRSGLLPKGPAMLCRLCWLVSYSLAVLLLGCLLFLRKAAKPAGDPTAHQPFWAPPT
  PRHSRCPPNHTVSSASLSLPSRHRLFLTYRHCRNFSILLEPSGCSKDTFLLLAIKSQPGHVERRAAIRST
  WGRVGGWARGRQLKLVFLLGVAGSAPPAQLLAYESREFDDILQWDFTEDFFNLTLKELHLQRWVVAACPQ
  AHFMLKGDDDVFVHVPNVLEFLDGWDPAQDLLVGDVIRQALPNRNTKVKYFIPPSMYRATHYPPYAGGGG
  YVMSRATVRRLQAIMEDAELFPIDDVFVGMCLRRLGLSPMHHAGFKTFGIRRPLDPLDPCLYRGLLLVHR
  LSPLEMWTMWALVTDEGLKCAAGPIPQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030765
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030765.4
• RefSeq Size: 1554 bp
• RefSeq ORF: 1137 bp
• Locus ID: 79369
• UniProt ID: Q9C0J1
• Cytogenetics: 12q24.31
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• MW: 42.3 kDa
• Gene Summary: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein. [provided by RefSeq, Jul 2008]