ULK2 (NM_014683) Human Tagged ORF Clone

CAT#: RC206010

ULK2 (Myc-DDK-tagged)-Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY428207) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227297 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ULK2
• Synonyms: ATG1B; Unc51.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206010 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGTGGGTGACTTCGAGTACAGCAAGAGGGATCTCGTGGGACACGGGGCCTTCGCCGTGGTCT
  TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTAGCTATTAAAAGTATTAATAAAAAGAACTTGTC
  AAAATCACAAATACTGCTTGGAAAGGAAATTAAAATCTTAAAGGAACTTCAGCATGAAAATATTGTAGCA
  CTCTATGATGTTCAGGAATTACCCAACTCTGTCTTTTTGGTGATGGAGTATTGCAATGGTGGAGACCTCG
  CAGATTATTTGCAAGCGAAAGGGACTCTCAGTGAAGACACGATCAGAGTGTTTCTGCATCAGATTGCTGC
  TGCCATGCGAATCCTGCACAGCAAAGGAATCATCCACAGAGATCTCAAACCACAGAACATCTTGCTGTCC
  TATGCCAATCGCAGAAAATCAAGTGTCAGTGGTATTCGCATCAAAATAGCGGATTTTGGTTTTGCTCGTT
  ACCTACATAGTAACATGATGGCTGCAACACTGTGTGGATCCCCGATGTACATGGCTCCTGAGGTTATTAT
  GTCTCAACATTATGATGCTAAGGCTGACTTGTGGAGCATAGGAACAGTGATATACCAATGCCTAGTTGGA
  AAACCACCTTTTCAGGCCAATAGTCCTCAAGACTTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC
  CTAGTATTCCCAGAGAAACATCACCTTATTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAACCAAAA
  AGATAGAATGGACTTTGAAGCGTTTTTTAGCCATCCTTTTCTTGAGCAAGGTCCAGTAAAAAAATCTTGC
  CCAGTTCCAGTGCCCATGTATTCTGGTTCTGTCTCTGGAAGCTCCTGTGGCAGCTCTCCATCTTGTCGTT
  TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCTTCCCCACCATTGGG
  TCCTCCCAACTATCTACAAGTTTCCAAAGATTCTGCCAGTACTAGTAGCAAGAACTCTTCTTGTGACACG
  GATGACTTTGTTTTGGTGCCACACAACATCTCGTCAGACCACTCATGTGATATGCCAATGGGGACTGCTG
  GCAGACGTGCTTCAAATGAATTCTTGGTGTGTGGAGGGCAGTGTCAGCCTACTGTGTCACCTCACAGCGA
  AACAGCACCAATTCCAGTTCCTACTCAAATAAGGAATTATCAGCGCATAGAGCAGAATCTTACATCTACT
  GCCAGCTCAGGCACAAATGTACATGGTTCTCCAAGATCTGCAGTGGTACGAAGGTCCAACACCAGCCCCA
  TGGGCTTCCTCCGGCCGGGATCATGCTCCCCAGTACCAGCAGACACAGCACAGACAGTTGGACGAAGGCT
  CTCCACTGGGTCTTCTAGGCCTTACTCACCTTCCCCTTTGGTTGGTACCATTCCTGAGCAATTCAGTCAG
  TGCTGCTGTGGGCATCCTCAGGGCCATGACTCCAGGAGTAGAAACTCCTCAGGTTCTCCAGTGCCACAAG
  CTCAGTCCCCACAGTCTCTCTTATCGGGTGCTAGACTGCAGAGCGCCCCCACCCTCACTGACATCTATCA
  GAACAAGCAGAAGCTCAGAAAACAGCACTCTGACCCCGTGTGCCCATCCCATACTGGGGCTGGGTACAGC
  TACTCGCCTCAGCCCAGTCGGCCTGGCAGCCTTGGAACTTCTCCCACCAAGCACTTGGGGTCCTCTCCAC
  GGAGTTCTGACTGGTTCTTTAAAACTCCTTTGCCAACAATCATTGGCTCTCCTACTAAGACCACAGCTCC
  TTTCAAAATCCCTAAAACTCAAGCATCTTCCAACCTGTTAGCCTTGGTTACTCGTCATGGGCCTGCTGAA
  GAACAGTCGAAAGATGGGAATGAGCCACGGGAATGTGCCCATTGCCTCTTAGTGCAAGGAAGTGAGAGGC
  AGCGGGCCGAGCAGCAGAGCAAGGCAGTGTTTGGCAGATCTGTCAGTACCGGGAAGTTATCAGATCAACA
  AGGAAAGACTCCTATATGTCGACATCAGGGCAGCACAGACAGTTTAAATACAGAACGACCAATGGATATA
  GCTCCGGCAGGAGCCTGTGGTGGTGTTCTGGCACCTCCTGCAGGTACAGCAGCAAGTTCCAAGGCTGTCC
  TCTTCACTGTAGGGTCTCCTCCACACAGTGCGGCAGCCCCCACTTGTACCCACATGTTCCTTCGAACAAG
  AACAACCTCAGTGGGGCCCAGCAACTCCGGGGGCTCTCTTTGTGCCATGAGTGGCCGCGTGTGCGTGGGG
  TCCCCGCCTGGCCCAGGCTTCGGCTCTTCCCCTCCAGGAGCAGAGGCAGCTCCCAGCCTGAGATACGTGC
  CTTACGGTGCTTCACCCCCCAGCCTAGAGGGGCTCATCACCTTTGAAGCCCCTGAACTGCCGGAGGAGAC
  GCTGATGGAGCGGGAACACACAGACACCTTACGCCATCTGAATGTGATGCTGATGTTCACTGAGTGTGTG
  CTGGACCTGACAGCCATGAGGGGAGGAAACCCTGAGCTGTGCACATCTGCTGTGTCCTTGTACCAGATCC
  AGGAGAGTGTGGTGGTGGACCAGATCAGTCAGCTGAGCAAAGACTGGGGGCGGGTGGAGCAGCTGGTGTT
  GTACATGAAAGCAGCACAGCTGCTTGCGGCTTCTCTGCATCTTGCCAAAGCCCAGATCAAGTCCGGGAAA
  CTGAGCCCATCCACAGCTGTGAAACAAGTTGTCAAGAATCTGAACGAACGATATAAATTCTGCATCACCA
  TGTGCAAGAAACTTACAGAAAAGCTGAATCGATTCTTCTCTGACAAACAGAGGTTTATTGATGAAATCAA
  CAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTAGAAATGGTTCAGTCTGCAGCCCTGGATGAG
  ATGTTTCAGCAGACCGAAGATATTGTTTATCGCTATCATAAGGCAGCCCTTCTTTTGGAAGGCCTAAGTA
  GGATTCTACAGGACCCTGCAGATATTGAAAATGTGCATAAATATAAATGTAGTATTGAGAGAAGACTGTC
  GGCGCTCTGCCATAGCACCGCAACCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206010 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVVGDFEYSKRDLVGHGAFAVVFRGRHRQKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVA
  LYDVQELPNSVFLVMEYCNGGDLADYLQAKGTLSEDTIRVFLHQIAAAMRILHSKGIIHRDLKPQNILLS
  YANRRKSSVSGIRIKIADFGFARYLHSNMMAATLCGSPMYMAPEVIMSQHYDAKADLWSIGTVIYQCLVG
  KPPFQANSPQDLRMFYEKNRSLMPSIPRETSPYLANLLLGLLQRNQKDRMDFEAFFSHPFLEQGPVKKSC
  PVPVPMYSGSVSGSSCGSSPSCRFASPPSLPDMQHIQEENLSSPPLGPPNYLQVSKDSASTSSKNSSCDT
  DDFVLVPHNISSDHSCDMPMGTAGRRASNEFLVCGGQCQPTVSPHSETAPIPVPTQIRNYQRIEQNLTST
  ASSGTNVHGSPRSAVVRRSNTSPMGFLRPGSCSPVPADTAQTVGRRLSTGSSRPYSPSPLVGTIPEQFSQ
  CCCGHPQGHDSRSRNSSGSPVPQAQSPQSLLSGARLQSAPTLTDIYQNKQKLRKQHSDPVCPSHTGAGYS
  YSPQPSRPGSLGTSPTKHLGSSPRSSDWFFKTPLPTIIGSPTKTTAPFKIPKTQASSNLLALVTRHGPAE
  EQSKDGNEPRECAHCLLVQGSERQRAEQQSKAVFGRSVSTGKLSDQQGKTPICRHQGSTDSLNTERPMDI
  APAGACGGVLAPPAGTAASSKAVLFTVGSPPHSAAAPTCTHMFLRTRTTSVGPSNSGGSLCAMSGRVCVG
  SPPGPGFGSSPPGAEAAPSLRYVPYGASPPSLEGLITFEAPELPEETLMEREHTDTLRHLNVMLMFTECV
  LDLTAMRGGNPELCTSAVSLYQIQESVVVDQISQLSKDWGRVEQLVLYMKAAQLLAASLHLAKAQIKSGK
  LSPSTAVKQVVKNLNERYKFCITMCKKLTEKLNRFFSDKQRFIDEINSVTAEKLIYNCAVEMVQSAALDE
  MFQQTEDIVYRYHKAALLLEGLSRILQDPADIENVHKYKCSIERRLSALCHSTATV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014683
• ORF Size: 3108 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014683.4
• RefSeq Size: 9165 bp
• RefSeq ORF: 3111 bp
• Locus ID: 9706
• UniProt ID: Q8IYT8
• Cytogenetics: 17p11.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway, Regulation of autophagy
• MW: 112.7 kDa
• Gene Summary: This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]