ULK2 (NM_014683) Human Untagged Clone

CAT#: SC323381

ULK2 (untagged)-Kinase deficient mutant (K39M) of Human unc-51-like kinase 2 (C. elegans) (ULK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ULK2
• Synonyms: ATG1B; Unc51.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014683, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGTGGTGGGTGACTTCGAGTACAGCAAGAGGGATCTCGTGGGACACGGGGCCTTCGCCGTGGTCT
  TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTAGCTATTAAAAGTATTAATAAAAAGAACTTGTC
  AAAATCACAAATACTGCTTGGAAAGGAAATTAAAATCTTAAAGGAACTTCAGCATGAAAATATTGTAGCA
  CTCTATGATGTTCAGGAATTACCCAACTCTGTCTTTTTGGTGATGGAGTATTGCAATGGTGGAGACCTCG
  CAGATTATTTGCAAGCGAAAGGGACTCTCAGTGAAGACACGATCAGAGTGTTTCTGCATCAGATTGCTGC
  TGCCATGCGAATCCTGCACAGCAAAGGAATCATCCACAGAGATCTCAAACCACAGAACATCTTGCTGTCC
  TATGCCAATCGCAGAAAATCAAGTGTCAGTGGTATTCGCATCAAAATAGCGGATTTTGGTTTTGCTCGTT
  ACCTACATAGTAACATGATGGCTGCAACACTGTGTGGATCCCCGATGTACATGGCTCCTGAGGTTATTAT
  GTCTCAACATTATGATGCTAAGGCTGACTTGTGGAGCATAGGAACAGTGATATACCAATGCCTAGTTGGA
  AAACCACCTTTTCAGGCCAATAGTCCTCAAGACTTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC
  CTAGTATTCCCAGAGAAACATCACCTTATTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAACCAAAA
  AGATAGAATGGACTTTGAAGCATTTTTTAGCCATCCTTTTCTTGAGCAAGGTCCAGTAAAAAAATCTTGC
  CCAGTTCCAGTGCCCATGTATTCTGGTTCTGTCTCTGGAAGCTCCTGTGGCAGCTCTCCATCTTGTCGTT
  TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCTTCCCCACCATTGGG
  TCCTCCCAACTATCTACAAGTTTCCAAAGATTCTGCCAGTACTAGTAGCAAGAACTCTTCTTGTGACACG
  GATGACTTTGTTTTGGTGCCACACAACATCTCGTCAGACCACTCATGTGATATGCCAGTGGGGACTGCTG
  GCAGACGTGCTTCAAATGAATTCTTGGTGTGTGGAGGGCAGTGTCAGCCTACTGTGTCACCTCACAGCGA
  AACAGCACCAATTCCAGTTCCTACTCAAATAAGGAATTATCAGCGCATAGAGCAGAATCTTACATCTACT
  GCCAGCTCAGGCACAAATGTACATGGTTCTCCAAGATCTGCAGTGGTACGAAGGTCCAACACCAGCCCCA
  TGGGCTTCCTCCGGCCGGGATCATGCTCCCCAGTACCAGCAGACACAGCACAGACAGTTGGACGAAGGCT
  CTCCACTGGGTCTTCTAGGCCTTACTCACCTTCCCCTTTGGTTGGTACCATTCCTGAGCAATTCAGTCAG
  TGCTGCTGTGGGCATCCTCAGGGCCATGACTCCAGGAGTAGAAACTCCTCAGGTTCTCCAGTGCCACAAG
  CTCAGTCCCCACAGTCTCTCTTATCGGGTGCTAGACTGCAGAGCGCCCCCACCCTCACTGACATCTATCA
  GAACAAGCAGAAGCTCAGAAAACAGCACTCTGACCCCGTGTGCCCATCCCATACTGGGGCTGGGTACAGC
  TACTCGCCTCAGCCCAGTCGGCCTGGCAGCCTTGGAACTTCTCCCACCAAGCACTTGGGGTCCTCTCCAC
  GGAGTTCTGACTGGTTCTTTAAAACTCCTTTGCCAACAATCATTGGCTCTCCTACTAAGACCACAGCTCC
  TTTCAAAATCCCTAAAACTCAAGCATCTTCCAACCTGTTAGCCTTGGTTACTCGTCATGGGCCTGCTGAA
  GAACAGTCGAAAGATGGGAATGAGCCACGGGAATGTGCCCATTGCCTCTTAGTGCAAGGAAGTGAGAGGC
  AGCGGGCCGAGCAGCAGAGCAAGGCAGTGTTTGGCAGATCTGTCAGTACCGGGAAGTTATCAGATCAACA
  AGGAAAGACTCCTATATGTCGACATCAGGGCAGCACAGACAGTTTAAATACAGAACGACCAATGGATATA
  GCTCCGGCAGGAGCCTGTGGTGGTGTTCTGGCACCTCCTGCAGGTACAGCAGCAAGTTCCAAGGCTGTCC
  TCTTCACTGTAGGGTCTCCTCCACACAGTGCGGCAGCCCCCACTTGTACCCACATGTTCCTTCGAACAAG
  AACAACCTCAGTGGGGCCCAGCAACTCCGGGGGCTCTCTTTGTGCCATGAGTGGCCGCGTGTGCGTGGGG
  TCCCCGCCTGGCCCAGGCTTCGGCTCTTCCCCTCCAGGAGCAGAGGCAGCTCCCAGCCTGAGATACGTGC
  CTTACGGTGCTTCACCCCCCAGCCTAGAGGGGCTCATCACCTTTGAAGCCCCTGAACTGCCGGAGGAGAC
  GCTGATGGAGCGGGAACACACAGACACCTTACGCCATCTGAATGTGATGCTGATGTTCACTGAGTGTGTG
  CTGGACCTGACAGCCATGAGGGGAGGAAACCCTGAGCTGTGCACATCTGCTGTGTCCTTGTACCAGATCC
  AGGAGAGTGTGGTGGTGGACCAGATCAGTCAGCTGAGCAAAGACTGGGGGCGGGTGGAGCAGCTGGTGTT
  GTACATGAAAGCAGCACAGCTGCTTGCGGCTTCTCTGCATCTTGCCAAAGCCCAGATCAAGTCCGGGAAA
  CTGAGCCCATCCACAGCTGTGAAACAAGTTGTCAAGAATCTGAACGAACGATATAAATTCTGCATCACCA
  TGTGCAAGAAACTTACAGAAAAGCTGAATCGATTCTTCTCTGACAAACAGAGGTTTATTGATGAAATCAA
  CAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTAGAAATGGTTCAGTCTGCAGCCCTGGATGAG
  ATGTTTCAGCAGACCGAAGATATTGTTTATCGCTATCATAAGGCAGCCCTTCTTTTGGAAGGCCTAAGTA
  GGATTCTACAGGACCCTGCAGATATTGAAAATGTGCATAAATATAAATGTAGTATTGAGAGAAGACTGTC
  GGCGCTCTGCCATAGCACCGCAACCGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014683 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGAGCGGGGAG
  GGCCGAGGAGGCCCGACGAGCTGGGGATGGAGAGTACCGGGCCCCTCACTGCCTCAGAGCGCGTGTGCGG
  CTCTGGGCGCGCACAGTGACGGTGACGGCACCCCTGGCCCGGCAGCGCCGAGGCCGCTTCGCCAGACAGC
  CAGCGGCCGGCGGCAGGCCGGGCCATGAGCGGCAGGGGCCGGGCCGGGCCTCGCTGACCCTGGCTTCGGC
  GCGGCAGCTTCCCCAGTTTCCGCTTCCGTCTCTCGGCATGAAGAGTCCGCCCGGCCCGGGGCTGGCGCTT
  GCCCAAAACCGGCCCGAGGCTGGCGCGCTCCGGGCCCGCGGAGCCGCGTTGCTGAATCCTGGCCCGAATG
  GCCGGCCCGCGGTCCGCGTGTTGCCCGGGGGCCGCGCCCTTGAAGGGGGGGTC
  
  Kinase domain sequence

  >SC323381 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGAGCGGGGAGGGCCGA
  GGAGGCCCGACGAGCTGGGGATGGAGAGTACCGGGCCCCTCACTGCCTCAGAGCGCGTGTGCGGCTCTGG
  GCGCGCACAGTGACGGTGACGGCACCCCTGGCCCGGCAGCGCCGA
  
• Restriction Sites: Please inquire
• ACCN: NM_014683
• Insert Size: 5730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014683.2, NP_055498.2
• RefSeq Size: 5807 bp
• RefSeq ORF: 3111 bp
• Locus ID: 9706
• UniProt ID: Q8IYT8
• Cytogenetics: 17p11.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway, Regulation of autophagy
• Gene Summary: This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.