GPR91 (SUCNR1) (NM_033050) Human Tagged ORF Clone

CAT#: RC205888

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SUCNR1 (Myc-DDK-tagged)-Human succinate receptor 1 (SUCNR1)



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SUCNR1
Synonyms GPR91
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205888 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGGGATCATGGCATGGAATGCAACTTGCAAAAACTGGCTGGCAGCAGAGGCTGCCCTGGAAAAGT
ACTACCTTTCCATTTTTTATGGGATTGAGTTCGTTGTGGGAGTCCTTGGAAATACCATTGTTGTTTACGG
CTACATCTTCTCTCTGAAGAACTGGAACAGCAGTAATATTTATCTCTTTAACCTCTCTGTCTCTGACTTA
GCTTTTCTGTGCACCCTCCCCATGCTGATAAGGAGTTATGCCAATGGAAACTGGATATATGGAGACGTGC
TCTGCATAAGCAACCGATATGTGCTTCATGCCAACCTCTATACCAGCATTCTCTTTCTCACTTTTATCAG
CATAGATCGATACTTGATAATTAAGTATCCTTTCCGAGAACACCTTCTGCAAAAGAAAGAGTTTGCTATT
TTAATCTCCTTGGCCATTTGGGTTTTAGTAACCTTAGAGTTACTACCCATACTTCCCCTTATAAATCCTG
TTATAACTGACAATGGCACCACCTGTAATGATTTTGCAAGTTCTGGAGACCCCAACTACAACCTCATTTA
CAGCATGTGTCTAACACTGTTGGGGTTCCTTATTCCTCTTTTTGTGATGTGTTTCTTTTATTACAAGATT
GCTCTCTTCCTAAAGCAGAGGAATAGGCAGGTTGCTACTGCTCTGCCCCTTGAAAAGCCTCTCAACTTGG
TCATCATGGCAGTGGTAATCTTCTCTGTGCTTTTTACACCCTATCACGTCATGCGGAATGTGAGGATCGC
TTCACGCCTGGGGAGTTGGAAGCAGTATCAGTGCACTCAGGTCGTCATCAACTCCTTTTACATTGTGACA
CGGCCTTTGGCCTTTCTGAACAGTGTCATCAACCCTGTCTTCTATTTTCTTTTGGGAGATCACTTCAGGG
ACATGCTGATGAATCAACTGAGACACAACTTCAAATCCCTTACATCCTTTAGCAGATGGGCTCATGAACT
CCTACTTTCATTCAGAGAAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205888 protein sequence
Red=Cloning site Green=Tags(s)

MLGIMAWNATCKNWLAAEAALEKYYLSIFYGIEFVVGVLGNTIVVYGYIFSLKNWNSSNIYLFNLSVSDL
AFLCTLPMLIRSYANGNWIYGDVLCISNRYVLHANLYTSILFLTFISIDRYLIIKYPFREHLLQKKEFAI
LISLAIWVLVTLELLPILPLINPVITDNGTTCNDFASSGDPNYNLIYSMCLTLLGFLIPLFVMCFFYYKI
ALFLKQRNRQVATALPLEKPLNLVIMAVVIFSVLFTPYHVMRNVRIASRLGSWKQYQCTQVVINSFYIVT
RPLAFLNSVINPVFYFLLGDHFRDMLMNQLRHNFKSLTSFSRWAHELLLSFREK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033050
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033050.6
RefSeq Size 1650 bp
RefSeq ORF 1005 bp
Locus ID 56670
UniProt ID Q9BXA5
Cytogenetics 3q25.1
Protein Families Druggable Genome, GPCR, Transmembrane
MW 38.7 kDa
Gene Summary This gene encodes a G-protein-coupled receptor for succinate, an intermediate molecule of the citric acid cycle. It is involved in the promotion of hematopoietic progenitor cell development, and it has a potential role in renovascular hypertension which has known correlations to renal failure, diabetes and atherosclerosis. [provided by RefSeq, Oct 2009]
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