Annexin V (ANXA5) (NM_001154) Human Tagged ORF Clone
ANXA5 (Myc-DDK-tagged)-Human annexin A5 (ANXA5)
|Product Name||Annexin V (ANXA5) (NM_001154) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ANX5; ENX2; HEL-S-7; PP4; RPRGL3|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC205619 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC205619 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||960 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001154.1, NM_001154.2, NM_001154.3, NP_001145.1|
|RefSeq Size||1624 bp|
|RefSeq ORF||963 bp|
|Gene Summary||The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications. [provided by RefSeq, Dec 2019]|
|The use of this cDNA Clones has been cited in the following citations:|
Annexin A5 regulates surface αvβ5 integrin for retinal clearance phagocytosis
,Yu, C;Muñoz, LE;Mallavarapu, M;Herrmann, M;Finnemann, SC;,
J. Cell. Sci. 2019
,PubMed ID 31515275
Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling
,Wang, J;Zhang, Y;Liu, X;Ma, J;Liu, P;Hu, C;Zhang, G;,
Oncol. Rep. Dec 2014
,PubMed ID 25323007
Annexin A5 promotes invasion and chemoresistance to temozolomide in glioblastoma multiforme cells
,Wu, L;Yang, L;Xiong, Y;Guo, H;Shen, X;Cheng, Z;Zhang, Y;Gao, Z;Zhu, X;,
Tumour Biol. Sep 2014
,PubMed ID 25245332
|cDNA Clone Resources|
|SC319544||ANXA5 (untagged)-Human annexin A5 (ANXA5)||
|RG205619||ANXA5 (GFP-tagged) - Human annexin A5 (ANXA5)||
|RC205619L3||Lenti ORF clone of Human annexin A5 (ANXA5), Myc-DDK-tagged||
|RC205619L4||Lenti ORF clone of Human annexin A5 (ANXA5), mGFP tagged||