WDR69 (DAW1) (NM_178821) Human Tagged ORF Clone

CAT#: RC205460

DAW1 (Myc-DDK-tagged)-Human WD repeat domain 69 (WDR69)

Product Images

Circular map for RC205460

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  Western blot validation of overexpression lysate (Cat# LY405839) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205460 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DAW1 protein (Cat# TP305460). The protein was produced from HEK293T cells transfected with DAW1 cDNA clone (Cat# RC205460) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR69
• Synonyms: ODA16; WDR69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205460 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCAAGAGCCTCCTGCTCCGGTATTACCCGCCAGGAATTATGTTGGAATATGAAAAACATGGAG
  AATTAAAGACTAAGTCCATAGATTTGCTTGATCTTGGTCCCAGCACTGATGTCAGTGCGTTAGTAGAAGA
  AATCCAGAAGGCAGAACCTCTACTCACAGCTTCACGAACAGAGCAAGTCAAACTTTTGATACAGAGGTTG
  CAAGAGAAACTCGGCCAGAACAGCAATCACACGTTCTATCTTTTTAAGGTTCTCAAAGCACATATATTGC
  CACTGACTAATGTTGCACTTAACAAATCGGGCTCATGCTTTATCACAGGAAGCTATGATCGGACGTGCAA
  GCTCTGGGACACTGCGTCTGGAGAGGAGCTGAACACGCTGGAGGGCCACAGGAATGTGGTTTATGCCATA
  GCATTCAACAATCCTTACGGTGACAAAATCGCCACTGGGTCCTTTGATAAAACTTGTAAACTCTGGAGTG
  TGGAAACAGGAAAATGTTACCATACCTTCAGGGGTCATACAGCAGAAATAGTGTGTTTATCATTTAACCC
  TCAAAGCACATTGGTGGCGACTGGAAGTATGGACACAACAGCCAAATTGTGGGACATTCAGAATGGCGAG
  GAAGTTTACACCTTAAGAGGACATTCTGCCGAAATCATCTCCTTGTCATTTAACACCTCAGGAGACAGAA
  TCATCACGGGGTCTTTTGATCATACCGTTGTAGTGTGGGACGCTGATACTGGAAGGAAGGTAAATATCTT
  AATTGGTCATTGTGCTGAGATTAGCAGTGCCTCATTCAATTGGGATTGCTCTCTAATATTAACTGGCTCT
  ATGGACAAAACCTGCAAGCTGTGGGATGCTACAAATGGAAAATGTGTGGCAACCTTAACAGGCCATGATG
  ATGAAATACTAGACAGCTGCTTTGATTACACTGGAAAGCTTATTGCAACTGCTTCAGCTGATGGAACAGC
  AAGAATTTTCAGTGCTGCCACAAGAAAATGCATTGCCAAACTGGAAGGTCATGAAGGTGAAATTTCAAAG
  ATTTCTTTCAACCCTCAAGGGAACCATCTTCTAACTGGCAGCTCTGACAAAACGGCTAGAATCTGGGATG
  CTCAGACTGGCCAGTGCCTCCAGGTTCTTGAGGGGCACACTGATGAAATCTTTTCATGTGCTTTCAACTA
  TAAAGGCAACATAGTCATTACAGGCAGCAAGGATAATACCTGTAGGATATGGCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205460 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLKSLLLRYYPPGIMLEYEKHGELKTKSIDLLDLGPSTDVSALVEEIQKAEPLLTASRTEQVKLLIQRL
  QEKLGQNSNHTFYLFKVLKAHILPLTNVALNKSGSCFITGSYDRTCKLWDTASGEELNTLEGHRNVVYAI
  AFNNPYGDKIATGSFDKTCKLWSVETGKCYHTFRGHTAEIVCLSFNPQSTLVATGSMDTTAKLWDIQNGE
  EVYTLRGHSAEIISLSFNTSGDRIITGSFDHTVVVWDADTGRKVNILIGHCAEISSASFNWDCSLILTGS
  MDKTCKLWDATNGKCVATLTGHDDEILDSCFDYTGKLIATASADGTARIFSAATRKCIAKLEGHEGEISK
  ISFNPQGNHLLTGSSDKTARIWDAQTGQCLQVLEGHTDEIFSCAFNYKGNIVITGSKDNTCRIWR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178821
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178821.3
• RefSeq Size: 1669 bp
• RefSeq ORF: 1248 bp
• Locus ID: 164781
• UniProt ID: Q8N136
• Cytogenetics: 2q36.3
• MW: 45.8 kDa
• Gene Summary: May play a role in axonemal outer row dynein assembly.[UniProtKB/Swiss-Prot Function]