Rb2 p130 (RBL2) (NM_005611) Human Tagged ORF Clone

CAT#: RC205380

RBL2 (Myc-DDK-tagged)-Human retinoblastoma-like 2 (p130) (RBL2)

Product Images

Western blot validation of overexpression lysate (Cat# LY417191) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205380 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RBL2 protein (Cat# TP305380). The protein was produced from HEK293T cells transfected with RBL2 cDNA clone (Cat# RC205380) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBL2
• Synonyms: P130; Rb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205380 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCGGGAGGTGACCAGTCGCCACCGCCCCCGCCTCCCCCTCCGGCGGCGGCAGCCTCGGATGAGG
  AGGAGGAGGACGACGGCGAGGCGGAAGACGCCGCGCCGCCTGCCGAGTCGCCCACCCCTCAGATCCAGCA
  GCGGTTCGACGAGCTGTGCAGCCGCCTCAACATGGACGAGGCGGCGCGGGCCGAGGCCTGGGACAGCTAC
  CGCAGCATGAGCGAAAGCTACACGCTGGAGGGAAATGATCTTCATTGGTTAGCATGTGCCTTATATGTGG
  CTTGCAGAAAATCTGTTCCAACTGTAAGCAAAGGGACAGTGGAAGGAAACTATGTATCTTTAACTAGAAT
  CCTGAAATGTTCAGAGCAGAGCTTAATCGAATTTTTTAATAAGATGAAGAAGTGGGAAGACATGGCAAAT
  CTACCCCCACATTTCAGAGAACGTACTGAGAGATTAGAAAGAAACTTCACTGTTTCTGCTGTAATTTTTA
  AGAAATATGAACCCATTTTTCAGGACATCTTTAAATACCCTCAAGAGGAGCAACCTCGTCAGCAGCGAGG
  AAGGAAACAGCGGCGACAGCCCTGTACTGTGTCTGAAATTTTCCATTTTTGTTGGGTGCTTTTTATATAT
  GCAAAAGGTAATTTCCCCATGATTAGTGATGATTTGGTCAATTCTTATCACCTGCTGCTGTGTGCTTTGG
  ACTTAGTTTATGGAAATGCACTTCAGTGTTCTAATCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCTT
  ATCTGAAGATTTTCATGCTAAAGATTCTAAACCTTCCTCTGACCCCCCTTGTATCATTGAGAAACTGTGT
  TCCTTACATGATGGCCTAGTTTTGGAAGCAAAGGGGATAAAGGAACATTTCTGGAAACCCTATATTAGGA
  AACTTTATGAAAAAAAGCTCCTTAAGGGAAAAGAAGAAAATCTCACTGGGTTTCTAGAACCTGGGAACTT
  TGGAGAGAGTTTTAAAGCCATCAATAAGGCCTATGAGGAGTATGTTTTATCTGTTGGGAATTTAGATGAG
  CGGATATTTCTTGGAGAGGATGCTGAGGAGGAAATTGGGACTCTCTCAAGGTGTCTGAACGCTGGTTCAG
  GAACAGAGACTGCTGAAAGGGTGCAGATGAAAAACATCTTACAGCAGCATTTTGACAAGTCCAAAGCACT
  TAGAATCTCCACACCACTAACTGGTGTTAGGTACATTAAGGAGAATAGCCCTTGTGTGACTCCAGTTTCT
  ACAGCTACGCATAGCTTGAGTCGTCTTCACACCATGCTGACAGGCCTCAGGAATGCACCAAGTGAGAAAC
  TGGAACAGATTCTCAGGACATGTTCCAGAGATCCAACCCAGGCTATTGCTAACAGACTGAAAGAAATGTT
  TGAAATATATTCTCAGCATTTCCAGCCAGACGAGGATTTCAGTAATTGTGCTAAAGAAATTGCCAGCAAA
  CATTTTCGTTTTGCGGAGATGCTTTACTATAAAGTATTAGAATCTGTTATTGAGCAGGAACAAAAAAGAC
  TAGGAGACATGGATTTATCTGGTATTCTGGAACAAGATGCGTTCCACAGATCTCTCTTGGCCTGCTGCCT
  TGAGGTCGTCACTTTTTCTTATAAGCCTCCTGGGAATTTTCCATTTATTACTGAAATATTTGATGTGCCT
  CTTTATCATTTTTATAAGGTGATAGAAGTATTCATTAGAGCAGAAGATGGCCTTTGTAGAGAGGTGGTAA
  AACACCTTAATCAGATTGAAGAACAGATCTTAGATCATTTGGCATGGAAACCAGAGTCTCCACTCTGGGA
  AAAAATTAGAGACAATGAAAACAGAGTTCCTACATGTGAAGAGGTCATGCCACCTCAGAACCTGGAAAGG
  GCAGATGAAATTTGCATTGCTGGCTCCCCTTTGACTCCCAGAAGGGTGACTGAAGTTCGTGCTGATACTG
  GAGGACTTGGAAGGAGCATAACATCTCCAACCACATTATACGATAGGTACAGCTCCCCACCAGCCAGCAC
  TACCAGAAGGCGGCTATTTGTTGAGAATGATAGCCCCTCTGATGGAGGGACGCCTGGGCGCATGCCCCCA
  CAGCCCCTAGTCAATGCTGTCCCTGTGCAGAATGTATCTGGGGAGACTGTTTCTGTCACACCAGTTCCTG
  GACAGACTTTGGTCACCATGGCAACCGCCACTGTCACAGCCAACAATGGGCAAACGGTAACCATTCCTGT
  GCAAGGTATTGCCAATGAAAATGGAGGGATAACATTCTTCCCTGTCCAAGTCAATGTTGGGGGGCAGGCA
  CAAGCTGTGACAGGCTCCATCCAGCCCCTCAGTGCTCAGGCCCTGGCTGGAAGTCTGAGCTCTCAACAGG
  TGACAGGAACAACTTTGCAAGTCCCTGGTCAAGTGGCCATTCAACAGATTTCCCCAGGTGGCCAACAGCA
  GAAGCAAGGCCAGTCTGTAACCAGCAGTAGTAATAGACCCAGGAAGACCAGCTCTTTATCGCTTTTCTTT
  AGAAAGGTATACCATTTAGCAGCTGTCCGCCTTCGGGATCTCTGTGCCAAACTAGATATTTCAGATGAAT
  TGAGGAAAAAAATCTGGACCTGCTTTGAATTCTCCATAATTCAGTGTCCTGAACTTATGATGGACAGACA
  TCTGGACCAGTTATTAATGTGTGCCATTTATGTGATGGCAAAGGTCACAAAAGAAGATAAGTCCTTCCAG
  AACATTATGCGTTGTTATAGGACTCAGCCGCAGGCCCGGAGCCAGGTGTATAGAAGTGTTTTGATAAAAG
  GAAAAAGAAAAAGAAGAAATTCTGGCAGCAGTGATAGCAGAAGCCATCAGAATTCTCCAACAGAACTAAA
  CAAAGATAGAACCAGTAGAGACTCCAGTCCAGTTATGAGGTCAAGCAGCACCTTGCCAGTTCCACAGCCC
  AGCAGTGCTCCTCCCACACCTACTCGCCTCACAGGTGCCAACAGTGACATGGAAGAAGAGGAGAGGGGAG
  ACCTCATTCAGTTCTACAACAACATCTACATCAAACAGATTAAGACATTTGCCATGAAGTACTCACAGGC
  AAATATGGATGCTCCTCCACTCTCTCCCTATCCATTTGTAAGAACAGGCTCCCCTCGCCGAATACAGTTG
  TCTCAAAATCATCCTGTCTACATTTCCCCACATAAAAATGAAACAATGCTTTCTCCTCGAGAAAAGATTT
  TCTATTACTTCAGCAACAGTCCTTCAAAGAGACTGAGAGAAATTAATAGTATGATACACACAGGAGAAAC
  TCCTACTAAAAAGAGAGGAATTCTTTTGGAAGATGGAAGTGAATCACCTGCAAAAAGAATTTGCCCAGAA
  AATCATTCTGCCTTATTACGCCGTCTCCAAGATGTAGCTAATGACCGTGGTTCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205380 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSY
  RSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMAN
  LPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIY
  AKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLC
  SLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDE
  RIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVS
  TATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASK
  HFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVP
  LYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLER
  ADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPP
  QPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQA
  QAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFF
  RKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQ
  NIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQP
  SSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQL
  SQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIHTGETPTKKRGILLEDGSESPAKRICPE
  NHSALLRRLQDVANDRGSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005611
• ORF Size: 3417 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005611.4
• RefSeq Size: 4903 bp
• RefSeq ORF: 3420 bp
• Locus ID: 5934
• UniProt ID: Q08999
• Cytogenetics: 16q12.2
• Domains: RB_B, RB_A, CYCLIN
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• MW: 128.3 kDa
• Gene Summary: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function]