Rb2 p130 (RBL2) (NM_005611) Human Untagged Clone

SKU SC317004

RBL2 (untagged)-Human retinoblastoma-like 2 (p130) (RBL2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Rb2 p130
• Synonyms: P130; Rb2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005611 edited; TTCGCCGTTTGAATGGCTGCGGGCCCGGGCCCTCACCTCACCTGAGGTCCGGCCGCCCAG; GGGTGCGCTATGCCGTCGGGAGGTGACCAGTCGCCACCGCCCCCGCCTCCCCCTCCGGCG; GCGGCAGCCTCGGATGAGGAGGAGGAGGACGACGGCGAGGCGGAAGACGCCGCGCCGCCT; GCCGAGTCGCCCACCCCTCAGATCCAGCAGCGGTTCGACGAGCTGTGCAGCCGCCTCAAC; ATGGACGAGGCGGCGCGGGCCGAGGCCTGGGACAGCTACCGCAGCATGAGCGAAAGCTAC; ACGCTGGAGGGAAATGATCTTCATTGGTTAGCATGTGCCTTATATGTGGCTTGCAGAAAA; TCTGTTCCAACTGTAAGCAAAGGGACAGTGGAAGGAAACTATGTATCTTTAACTAGAATC; CTGAAATGTTCAGAGCAGAGCTTAATCGAATTTTTTAATAAGATGAAGAAGTGGGAAGAC; ATGGCAAATCTACCCCCACATTTCAGAGAACGTACTGAGAGATTAGAAAGAAACTTCACT; GTTTCTGCTGTAATTTTTAAGAAATATGAACCCATTTTTCAGGACATCTTTAAATACCCT; CAAGAGGAGCAACCTCGTCAGCAGCGAGGAAGGAAACAGCGGCGACAGCCCTGTACTGTG; TCTGAAATTTTCCATTTTTGTTGGGTGCTTTTTATATATGCAAAAGGTAATTTCCCCATG; ATTAGTGATGATTTGGTCAATTCTTATCACCTGCTGCTGTGTGCTTTGGACTTAGTTTAT; GGAAATGCACTTCAGTGTTCTAATCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCTTA; TCTGAAGATTTTCATGCTAAAGATTCTAAACCTTCCTCTGACCCCCCTTGTATCATTGAG; AAACTGTGTTCCTTACATGATGGCCTAGTTTTGGAAGCAAAGGGGATAAAGGAACATTTC; TGGAAACCCTATATTAGGAAACTTTATGAAAAAAAGCTCCTTAAGGGAAAAGAAGAAAAT; CTCACTGGGTTTCTAGAACCTGGGAACTTTGGAGAGAGTTTTAAAGCCATCAATAAGGCC; TATGAGGAGTATGTTTTATCTGTTGGGAATTTAGATGAGCGGATATTTCTTGGAGAGGAT; GCTGAGGAGGAAATTGGGACTCTCTCAAGGTGTCTGAACGCTGGTTCAGGAACAGAGACT; GCTGAAAGGGTGCAGATGAAAAACATCTTACAGCAGCATTTTGACAAGTCCAAAGCACTT; AGAATCTCCACACCACTAACTGGTGTTAGGTACATTAAGGAGAATAGCCCTTGTGTGACT; CCAGTTTCTACAGCTACGCATAGCTTGAGTCGTCTTCACACCATGCTGACAGGCCTCAGG; AATGCACCAAGTGAGAAACTGGAACAGATTCTCAGGACATGTTCCAGAGATCCAACCCAG; GCTATTGCTAACAGACTGAAAGAAATGTTTGAAATATATTCTCAGCATTTCCAGCCAGAC; GAGGATTTCAGTAATTGTGCTAAAGAAATTGCCAGCAAACATTTTCGTTTTGCGGAGATG; CTTTACTATAAAGTATTAGAATCTGTTATTGAGCAGGAACAAAAAAGACTAGGAGACATG; GATTTATCTGGTATTCTGGAACAAGATGCGTTCCACAGATCTCTCTTGGCCTGCTGCCTT; GAGGTCGTCACTTTTTCTTATAAGCCTCCTGGGAATTTTCCATTTATTACTGAAATATTT; GATGTGCCTCTTTATCATTTTTATAAGGTGATAGAAGTATTCATTAGAGCAGAAGATGGC; CTTTGTAGAGAGGTGGTAAAACACCTTAATCAGATTGAAGAACAGATCTTAGATCATTTG; GCATGGAAACCAGAGTCTCCACTCTGGGAAAAAATTAGAGACAATGAAAACAGAGTTCCT; ACATGTGAAGAGAACCTGGAAAGGGCAGATGAAATTTGCATTGCTGGCTCCCCTTTGACT; CCCAGAAGGGTGACTGAAGTTCGTGCTGATACTGGAGGACTTGGAAGGAGCATAACATCT; CCAACCACATTATACGATAGGTACAGCTCCCCACCAGCCAGCACTACCAGAAGGCGGCTA; TTTGTTGAGAATGATAGCCCCTCTGATGGAGGGACGCCTGGGCGCATGCCCCCACAGCCC; CTAGTCAATGCTGTCCCTGTGCAGAATGTATCTGGGGAGACTGTTTCTGTCACACCAGTT; CCTGGACAGACTTTGGTCACCATGGCAACCGCCACTGTCACAGCCAACAATGGGCAAACG; GTAACCATTCCTGTGCAAGGTATTGCCAATGAAAATGGAGGGATAACATTCTTCCCTGTC; CAAGTCAATGTTGGGGGGCAGGCACAAGCTGTGACAGGCTCCATCCAGCCCCTCAGTGCT; CAGGCCCTGGCTGGAAGTCTGAGCTCTCAACAGGTGACAGGAACAACTTTGCAAGTCCCT; GGTCAAGTGGCCATTCAACAGATTTCCCCAGGTGGCCAACAGCAGAAGCAAGGCCAGTCT; GTAACCAGCAGTAGTAATAGACCCAGGAAGACCAGCTCTTTATCGCTTTTCTTTAGAAAG; GTATACCATTTAGCAGCTGTCCGCCTTCGGGATCTCTGTGCCAAACTAGATATTTCAGAT; GAATTGAGGAAAAAAATCTGGACCTGCTTTGAATTCTCCATAATTCAGTGTCCTGAACTT; ATGATGGACAGACATCTGGACCAGTTATTAATGTGTGCCATTTATGTGATGGCAAAGGTC; ACAAAAGAAGATAAGTCCTTCCAGAACATTATGCGTTGTTATAGGACTCAGCCGCAGGCC; CGGAGCCAGGTGTATAGAAGTGTTTTGATAAAAGGGAAAAGAAAAAGAAGAAATTCTGGC; AGCAGTGATAGCAGAAGCCATCAGAATTCTCCAACAGAACTAAACAAAGATAGAACCAGT; AGAGACTCCAGTCCAGTTATGAGGTCAAGCAGCACCTTGCCAGTTCCACAGCCCAGCAGT; GCTCCTCCCACACCTACTCGCCTCACAGGTGCCAACAGTGACATGGAAGAAGAGGAGAGG; GGAGACCTCATTCAGTTCTACAACAACATCTACATCAAACAGATTAAGACATTTGCCATG; AAGTACTCACAGGCAAATATGGATGCTCCTCCACTCTCTCCCTATCCATTTGTAAGAACA; GGCTCCCCTCGCCGAATACAGTTGTCTCAAAATCATCCTGTCTACATTTCCCCACATAAA; AATGAAACAATGCTTTCTCCTCGAGAAAAGATTTTCTATTACTTCAGCAACAGTCCTTCA; AAGAGACTGAGAGAAATTAATAGTATGATACGCACAGGAGAAACTCCTACTAAAAAGAGA; GGAATTCTTTTGGAAGATGGAAGTGAATCACCTGCAAAAAGAATTTGCCCAGAAAATCAT; TCTGCCTTATTACGCCGTCTCCAAGATGTAGCTAATGACCGTGGTTCCCACTGAGGTTAG; TCTCTTGTATTAAACTCTTCACAAAATCTGTTTAGCAGCAGCCTTTAATGCATCTAGATT; ATGGAGCTTTTTTCCTTAATCCAGCTGATGAGTTACAGCCTGTTAGTAACATGAGGGGAC; ATTTTGGTGAGAAATGGGACTTAACTCCTTCCAGTGTCCTTAGAACATTTTAATTCATCC; CAACTGTCTTTTTTTCCCTACCATTCAGTGATTACTGTCAAGGCTGCTTACAATCCAAAC; TTGGATTTTTGACTCTGGCAAAGCTTTTAGAAATACTGCAAGAAAATGATGTGTACCCAA; ACGTGAGCATAGGAGGCTTCTGTTGACGTACTCCAACAGAAGAACTGTGTTTCAAGTTCA; ATCCTACCTGTTTTGTGGTCAGCTGTAGTCCTCATAAAAAGCAAAACAAAAATTAGGTAT; TTTGTCCTAAAACACCTGGTAGGAGTGTGTGATTTTTTGCATTCCTGACAAAGGAGAGCA; CACCCAGGTTTGGAGGTCCTAGGTCATTAGCCCTCGTCTCCCGTTCCCTTTGTGCACATC; TTCCCTCTCCCCATTCGGTGTGGTGCAGTGTGAAAAGTCCTTGATTGTTCGGGTGTGCAA; TGTCTGAGTGAACCTGTATAAGTGGAGGCACTTTAGGGCTGTAAAATGCATGATTTTGTA; ACCCAGATTTTGCTGTATATTTGTGATAGCACTTTCTACAATGTGAACTTTATTAAATAC; AAAACTTCCAGGCTAAACATCCAATATTTTCTTTAATGCTTTTATATTTTTTTAAAATGT; TAAAACCCCTATAGCCACCTTTTGGGAATGTTTTAAATTCTCCAGTTTTTTGTTATATAG; GGATCAACCAGCTAAGAAAAGATTTTAATCAAGTTGAATTGAGGGGATTAATATGAAAAC; TTATGACCTCTTCCTTTAGGAGGGAGTTATCTAAAAGAAATGTCTATTAAGGTGATATAT; TTAAAAATATTTTTGGGTGTTCCTGGCAGTTTAAAAAAATTGGTTGGAGAATTTAGGTTT; TTATTAGTACCATAGTACCATTTATACAAATTAGAAAATGTTATTTAACAGCTGAATTAT; CTATACATATCTTTATTAATCACTATTGTTCCAGCAGTTTTCAAGTCAAATTAATAATCT; TATTAGGGAGAAAATTCAATTGTAAATTGAATCAGTATAAACAAAGTTACTAGGTAACTT; CATATTGCTGAGAGAAATATGGAACTTACATTGTTCAATTAGAATAGTGTTCTGCAAAAA; TATTTATAAAACTTCTCAAGATACTGCTACTGTAATTTTATATGAAGATAAGTGTATTTT; TCAATAAAGCATTTATAAATTAAAAAAAAAAAAAAAAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005611
• Insert Size: 4800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a good match to the protein associated to this reference except for a 12bp deletion.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005611.2, NP_005602.2
• RefSeq Size: 4853 bp
• RefSeq ORF: 3420 bp
• Locus ID: 5934
• UniProt ID: Q08999
• Cytogenetics: 16q12.2
• Domains: CYCLIN, RB_A, RB_B
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• Summary: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function]