SLC22A8 (NM_004254) Human Tagged ORF Clone

CAT#: RC205056

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SLC22A8 (Myc-DDK-tagged)-Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1



  "NM_004254" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SLC22A8
Synonyms OAT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205056 representing NM_004254
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTTCTCGGAGATCCTGGACCGTGTGGGAAGCATGGGCCATTTCCAGTTCCTGCATGTAGCCATAC
TGGGCCTCCCGATCCTCAACATGGCCAACCACAACCTGCTGCAGATCTTCACAGCCGCCACCCCTGTCCA
CCACTGTCGCCCGCCCCACAATGCCTCCACAGGGCCTTGGGTGCTCCCCATGGGCCCAAATGGGAAGCCT
GAGAGGTGCCTCCGTTTTGTACATCCGCCCAATGCCAGCCTGCCCAATGACACCCAGAGGGCCATGGAGC
CATGCCTGGATGGCTGGGTCTACAACAGCACCAAGGACTCCATTGTGACAGAGTGGGACTTGGTGTGCAA
CTCCAACAAACTGAAGGAGATGGCCCAGTCTATCTTCATGGCAGGTATACTGATTGGAGGGCTCGTGCTT
GGAGACCTGTCTGACAGGTTTGGCCGCAGGCCCATCCTGACCTGCAGCTACCTGCTGCTGGCAGCCAGCG
GCTCCGGTGCAGCCTTCAGCCCCACCTTCCCCATCTACATGGTCTTCCGCTTCCTGTGTGGCTTTGGCAT
CTCAGGCATTACCCTGAGCACCGTCATCTTGAATGTGGAATGGGTGCCTACCCGGATGCGGGCCATCATG
TCGACAGCACTCGGGTACTGCTACACCTTTGGCCAGTTCATTCTGCCCGGCCTGGCCTACGCCATCCCCC
AGTGGCGTTGGCTGCAGTTAACTGTGTCCATTCCCTTCTTCGTCTTCTTCCTATCATCCTGGTGGACACC
AGAGTCCATACGCTGGTTGGTCTTGTCTGGAAAGTCCTCGAAGGCCCTGAAGATACTCCGGCGGGTGGCT
GTCTTCAATGGCAAGAAGGAAGAGGGAGAAAGGCTCAGCTTGGAGGAGCTCAAACTCAACCTGCAGAAGG
AGATCTCCTTGGCCAAGGCCAAGTACACCGCAAGTGACCTGTTCCGGATACCCATGCTGCGCCGCATGAC
CTTCTGTCTTTCCCTGGCCTGGTTTGCTACCGGTTTTGCCTACTATAGTTTGGCTATGGGTGTGGAAGAA
TTTGGAGTCAACCTCTACATCCTCCAGATCATCTTTGGTGGGGTCGATGTCCCAGCCAAGTTCATCACCA
TCCTCTCCTTAAGCTACCTGGGCCGGCATACCACTCAGGCCGCTGCCCTGCTCCTGGCAGGAGGGGCCAT
CTTGGCTCTCACCTTTGTGCCCTTGGACTTGCAGACCGTGAGGACAGTATTGGCTGTGTTTGGGAAGGGA
TGCCTATCCAGCTCCTTCAGCTGCCTCTTCCTCTACACAAGTGAATTATACCCCACAGTCATCAGGCAAA
CAGGTATGGGCGTAAGTAACCTGTGGACCCGCGTGGGAAGCATGGTGTCCCCGCTGGTGAAAATCACGGG
TGAGGTACAGCCCTTCATCCCCAATATCATCTACGGGATCACCGCCCTCCTCGGGGGCAGTGCTGCCCTC
TTCCTGCCTGAGACCCTGAATCAGCCCTTGCCAGAGACTATCGAAGACCTGGAAAACTGGTCCCTGCGGG
CAAAGAAGCCAAAGCAGGAGCCAGAGGTGGAAAAGGCCTCCCAGAGGATCCCTCTACAGCCTCACGGACC
AGGCCTGGGCTCCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205056 representing NM_004254
Red=Cloning site Green=Tags(s)

MTFSEILDRVGSMGHFQFLHVAILGLPILNMANHNLLQIFTAATPVHHCRPPHNASTGPWVLPMGPNGKP
ERCLRFVHPPNASLPNDTQRAMEPCLDGWVYNSTKDSIVTEWDLVCNSNKLKEMAQSIFMAGILIGGLVL
GDLSDRFGRRPILTCSYLLLAASGSGAAFSPTFPIYMVFRFLCGFGISGITLSTVILNVEWVPTRMRAIM
STALGYCYTFGQFILPGLAYAIPQWRWLQLTVSIPFFVFFLSSWWTPESIRWLVLSGKSSKALKILRRVA
VFNGKKEEGERLSLEELKLNLQKEISLAKAKYTASDLFRIPMLRRMTFCLSLAWFATGFAYYSLAMGVEE
FGVNLYILQIIFGGVDVPAKFITILSLSYLGRHTTQAAALLLAGGAILALTFVPLDLQTVRTVLAVFGKG
CLSSSFSCLFLYTSELYPTVIRQTGMGVSNLWTRVGSMVSPLVKITGEVQPFIPNIIYGITALLGGSAAL
FLPETLNQPLPETIEDLENWSLRAKKPKQEPEVEKASQRIPLQPHGPGLGSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004254
ORF Size 1626 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004254.4
RefSeq Size 2207 bp
RefSeq ORF 1629 bp
Locus ID 9376
UniProt ID Q8TCC7
Cytogenetics 11q12.3
Protein Families Transmembrane
MW 59.7 kDa
Gene Summary This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, May 2010]

Other Versions

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