USF1 (NM_007122) Human Tagged ORF Clone

CAT#: RC204915

USF1 (Myc-DDK-tagged)-Human upstream transcription factor 1 (USF1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416182) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204915 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified USF1 protein (Cat# TP304915). The protein was produced from HEK293T cells transfected with USF1 cDNA clone (Cat# RC204915) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USF1
• Synonyms: bHLHb11; FCHL; FCHL1; HYPLIP1; MLTF; MLTFI; UEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204915 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGGCAGCAGAAAACAGCTGAAACGGAAGAGGGGACAGTGCAGATTCAGGAAGGTGCAGTGGCTA
  CTGGGGAAGACCCAACCAGTGTGGCTATTGCCAGCATCCAGTCAGCTGCCACCTTCCCTGACCCCAACGT
  CAAGTACGTCTTCCGAACTGAGAATGGGGGCCAGGTGATGTACAGGGTGATCCAGGTGTCTGAGGGGCAG
  CTGGATGGCCAAACTGAGGGAACTGGCGCCATCAGTGGCTACCCTGCCACTCAATCCATGACCCAGGCGG
  TGATCCAGGGTGCTTTCACCAGTGATGATGCAGTTGACACGGAGGGGACAGCTGCTGAGACGCACTATAC
  TTACTTCCCCAGCACGGCAGTGGGAGATGGGGCAGGGGGTACCACATCGGGGAGTACAGCTGCTGTTGTT
  ACTACCCAGGGCTCAGAGGCACTGCTGGGGCAGGCGACCCCTCCTGGCACTGGTCAATTCTTTGTGATGA
  TGTCACCACAAGAAGTACTGCAGGGAGGAAGCCAGCGCTCAATTGCCCCTAGGACTCACCCTTATTCCCC
  GAAGTCAGAAGCTCCCCGGACGACTCGGGATGAGAAACGCAGGGCTCAGCATAATGAAGTGGAGCGTCGC
  CGCCGAGACAAGATCAACAACTGGATCGTGCAGCTCTCCAAGATAATCCCAGACTGCTCTATGGAGAGCA
  CCAAGTCTGGCCAGAGTAAAGGTGGGATTCTATCCAAAGCTTGTGATTATATCCAGGAGCTTCGGCAGAG
  TAACCACCGCTTGTCTGAAGAACTGCAGGGACTTGACCAACTGCAGCTGGACAATGACGTGCTTCGACAA
  CAGGTGGAAGATCTTAAAAACAAGAATCTGCTGCTTCGAGCTCAGTTGCGGCACCACGGATTAGAGGTCG
  TCATCAAGAATGACAGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204915 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQ
  LDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVV
  TTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERR
  RRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQ
  QVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007122
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007122.5
• RefSeq Size: 1830 bp
• RefSeq ORF: 933 bp
• Locus ID: 7391
• UniProt ID: P22415
• Cytogenetics: 1q23.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 33.5 kDa
• Gene Summary: This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]