PCK1 (NM_002591) Human Tagged Lenti ORF Clone
CAT#: RC204758L4
- LentiORF®
Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), mGFP tagged
"NM_002591" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | PCK1 |
Synonyms | PCKDC; PEPCK-C; PEPCK1; PEPCKC |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC204758).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002591 |
ORF Size | 1866 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002591.2 |
RefSeq Size | 2692 bp |
RefSeq ORF | 1869 bp |
Locus ID | 5105 |
UniProt ID | P35558 |
Cytogenetics | 20q13.31 |
Domains | PEPCK |
Protein Families | Druggable Genome |
Protein Pathways | Adipocytokine signaling pathway, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism |
MW | 69.2 kDa |
Gene Summary | This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204758 | PCK1 (Myc-DDK-tagged)-Human phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1) |
USD 579.00 |
|
RC204758L1 | Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), Myc-DDK-tagged |
USD 879.00 |
|
RC204758L2 | Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), mGFP tagged |
USD 879.00 |
|
RC204758L3 | Lenti ORF clone of Human phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), Myc-DDK-tagged |
USD 879.00 |
|
RG204758 | PCK1 (tGFP-tagged) - Human phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1) |
USD 779.00 |
|
SC319751 | PCK1 (untagged)-Human phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1) |
USD 580.00 |
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