COQ6 (NM_182476) Human Tagged ORF Clone

CAT#: RC204353

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COQ6 (Myc-DDK-tagged)-Human coenzyme Q6 homolog, monooxygenase (S. cerevisiae) (COQ6), nuclear gene encoding mitochondrial protein, transcript variant 1



  "NM_182476" in other vectors (4)

USD 433.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Product Name COQ6 (NM_182476) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol COQ6
Synonyms CGI-10; CGI10; COQ10D6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC204353 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCCGGCTTGTCAGCCGATGCGGGGCTGTGCGTGCAGCTCCCCACAGCGGCCCGCTGGTGTCCT
GGCGCAGGTGGTCCGGCGCCTCAACAGACACCGTGTATGACGTGGTGGTGTCGGGTGGAGGCCTGGTGGG
CGCTGCCATGGCCTGTGCCTTGGGATATGATATTCACTTTCATGACAAGAAAATCCTGTTGCTCGAAGCA
GGTCCAAAGAAAGTACTGGAGAAATTGTCAGAAACTTACAGCAACAGGGTCAGCTCCATTTCCCCTGGCT
CTGCAACGCTTCTCAGTAGTTTTGGTGCCTGGGACCATATCTGCAACATGAGATACAGAGCCTTTCGGCG
AATGCAGGTGTGGGACGCCTGCTCAGAGGCCCTGATAATGTTTGATAAGGATAATTTAGATGACATGGGC
TATATCGTGGAGAATGATGTCATCATGCATGCTCTCACTAAGCAGTTGGAGGCTGTGTCTGACCGAGTGA
CGGTTCTCTACAGGAGCAAAGCCATTCGCTATACCTGGCCTTGTCCATTTCCTATGGCCGACTCCAGCCC
TTGGGTTCATATTACCCTAGGTGATGGCAGCACCTTCCAGACCAAATTGTTGATAGGTGCAGATGGTCAC
AACTCCGGAGTACGGCAGGCTGTTGGAATCCAGAATGTGAGCTGGAACTATGACCAGTCTGCTGTTGTGG
CTACTCTGCATTTATCAGAGGCCACAGAAAACAACGTAGCCTGGCAGAGATTTCTTCCCTCTGGGCCTAT
TGCTCTGCTCCCGCTCTCAGACACCTTGAGTTCCTTGGTTTGGTCCACGTCCCATGAACATGCAGCAGAG
CTAGTTAGCATGGATGAGGAAAAATTTGTGGATGCCGTTAACTCTGCCTTTTGGAGTGATGCTGACCACA
CGGACTTCATCGACACAGCTGGTGCCATGCTGCAGTATGCTGTCAGCCTTCTGAAGCCCACTAAGGTCTC
GGCTCGCCAGCTGCCCCCAAGCGTAGCCAGGGTGGATGCCAAAAGCCGAGTTCTGTTTCCTCTTGGGTTG
GGACATGCTGCTGAGTACGTCAGGCCTCGGGTGGCGCTCATTGGGGATGCAGCCCACAGAGTCCATCCGC
TTGCAGGACAGGGTGTCAACATGGGCTTTGGGGATATCTCCAGCTTGGCCCATCACCTCAGTACGGCAGC
CTTCAATGGGAAGGACTTAGGTTCCGTGAGCCACCTCACAGGTTATGAAACAGAAAGACAGCGTCACAAC
ACTGCTCTTCTGGCTGCTACAGACTTACTAAAAAGGCTCTATTCTACCAGTGCCTCCCCGCTTGTGTTGC
TCAGGACGTGGGGCTTGCAGGCCACAAATGCAGTGTCTCCACTCAAAGAACAGATTATGGCCTTTGCAAG
CAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204353 protein sequence
Red=Cloning site Green=Tags(s)

MAARLVSRCGAVRAAPHSGPLVSWRRWSGASTDTVYDVVVSGGGLVGAAMACALGYDIHFHDKKILLLEA
GPKKVLEKLSETYSNRVSSISPGSATLLSSFGAWDHICNMRYRAFRRMQVWDACSEALIMFDKDNLDDMG
YIVENDVIMHALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGH
NSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAE
LVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGL
GHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHN
TALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_182476
ORF Size 1404 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_182476.3
RefSeq Size 1615 bp
RefSeq ORF 1407 bp
Locus ID 51004
Cytogenetics 14q24.3
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Ubiquinone and other terpenoid-quinone biosynthesis
MW 50.9 kDa
Gene Summary The protein encoded by this gene belongs to the ubiH/COQ6 family. It is an evolutionarily conserved monooxygenase required for the biosynthesis of coenzyme Q10 (or ubiquinone), which is an essential component of the mitochondrial electron transport chain, and one of the most potent lipophilic antioxidants implicated in the protection of cell damage by reactive oxygen species. Knockdown of this gene in mouse and zebrafish results in decreased growth due to increased apoptosis. Mutations in this gene are associated with autosomal recessive coenzyme Q10 deficiency-6 (COQ10D6), which manifests as nephrotic syndrome with sensorineural deafness. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2012]

Other Versions

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