ARSA (NM_000487) Human Tagged ORF Clone

CAT#: RC204319

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ARSA (Myc-DDK-tagged)-Human arylsulfatase A (ARSA), transcript variant 1



  "NM_000487" in other vectors (7)

USD 420.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name ARSA (NM_000487) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ARSA
Synonyms ASA; MLD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC204319 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCACCGCGGTCCCTCCTCCTGGCCCTGGCTGCTGGCCTGGCCGTTGCCCGTCCGCCCAACATCG
TGCTGATCTTTGCCGACGACCTCGGCTATGGGGACCTGGGCTGCTATGGGCACCCCAGCTCTACCACTCC
CAACCTGGACCAGCTGGCGGCGGGAGGGCTGCGGTTCACAGACTTCTACGTGCCTGTGTCTCTGTGCACA
CCCTCTAGGGCCGCCCTCCTGACCGGCCGGCTCCCGGTTCGGATGGGCATGTACCCTGGCGTCCTGGTGC
CCAGCTCCCGGGGGGGCCTGCCCCTGGAGGAGGTGACCGTGGCCGAAGTCCTGGCTGCCCGAGGCTACCT
CACAGGAATGGCCGGCAAGTGGCACCTTGGGGTGGGGCCTGAGGGGGCCTTCCTGCCCCCCCATCAGGGC
TTCCATCGATTTCTAGGCATCCCGTACTCCCACGACCAGGGCCCCTGCCAGAACCTGACCTGCTTCCCGC
CGGCCACTCCTTGCGACGGTGGCTGTGACCAGGGCCTGGTCCCCATCCCACTGTTGGCCAACCTGTCCGT
GGAGGCGCAGCCCCCCTGGCTGCCCGGACTAGAGGCCCGCTACATGGCTTTCGCCCATGACCTCATGGCC
GACGCCCAGCGCCAGGATCGCCCCTTCTTCCTGTACTATGCCTCTCACCACACCCACTACCCTCAGTTCA
GTGGGCAGAGCTTTGCAGAGCGTTCAGGCCGCGGGCCATTTGGGGACTCCCTGATGGAGCTGGATGCAGC
TGTGGGGACCCTGATGACAGCCATAGGGGACCTGGGGCTGCTTGAAGAGACGCTGGTCATCTTCACTGCA
GACAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCTTGCGGTGTGGAAAGGGAA
CGACCTACGAGGGCGGTGTCCGAGAGCCTGCCTTGGCCTTCTGGCCAGGTCATATCGCTCCCGGCGTGAC
CCACGAGCTGGCCAGCTCCCTGGACCTGCTGCCTACCCTGGCAGCCCTGGCTGGGGCCCCACTGCCCAAT
GTCACCTTGGATGGCTTTGACCTCAGCCCCCTGCTGCTGGGCACAGGCAAGAGCCCTCGGCAGTCTCTCT
TCTTCTACCCGTCCTACCCAGACGAGGTCCGTGGGGTTTTTGCTGTGCGGAGTGGAAAGTACAAGGCTCA
CTTCTTCACCCAGGGCTCTGCCCACAGTGATACCACTGCAGACCCTGCCTGCCACGCCTCCAGCTCTCTG
ACTGCTCATGAGCCCCCGCTGCTCTATGACCTGTCCAAGGACCCTGGTGAGAACTACAACCTGCTGGGGG
GTGTGGCCGGGGCCACCCCAGAGGTGCTGCAAGCCCTGAAACAGCTTCAGCTGCTCAAGGCCCAGTTAGA
CGCAGCTGTGACCTTCGGCCCCAGCCAGGTGGCCCGGGGCGAGGACCCCGCCCTGCAGATCTGCTGTCAT
CCTGGCTGCACCCCCCGCCCAGCTTGCTGCCATTGCCCAGATCCCCATGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204319 protein sequence
Red=Cloning site Green=Tags(s)

MGAPRSLLLALAAGLAVARPPNIVLIFADDLGYGDLGCYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCT
PSRAALLTGRLPVRMGMYPGVLVPSSRGGLPLEEVTVAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQG
FHRFLGIPYSHDQGPCQNLTCFPPATPCDGGCDQGLVPIPLLANLSVEAQPPWLPGLEARYMAFAHDLMA
DAQRQDRPFFLYYASHHTHYPQFSGQSFAERSGRGPFGDSLMELDAAVGTLMTAIGDLGLLEETLVIFTA
DNGPETMRMSRGGCSGLLRCGKGTTYEGGVREPALAFWPGHIAPGVTHELASSLDLLPTLAALAGAPLPN
VTLDGFDLSPLLLGTGKSPRQSLFFYPSYPDEVRGVFAVRSGKYKAHFFTQGSAHSDTTADPACHASSSL
TAHEPPLLYDLSKDPGENYNLLGGVAGATPEVLQALKQLQLLKAQLDAAVTFGPSQVARGEDPALQICCH
PGCTPRPACCHCPDPHA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_000487
ORF Size 1521 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000487.1, NM_000487.2, NM_000487.3, NM_000487.4, NM_000487.5, NP_000478.2
RefSeq Size 4325 bp
RefSeq ORF 1530 bp
Locus ID 410
Cytogenetics 22q13.33
Domains Sulfatase
Protein Families Druggable Genome
Protein Pathways Lysosome, Sphingolipid metabolism
MW 53.6 kDa
Gene Summary The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010]

Citations (1)

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