Cystatin A (CSTA) (NM_005213) Human Tagged ORF Clone
CSTA (Myc-DDK-tagged)-Human cystatin A (stefin A) (CSTA)
|Type||Human Tagged ORF Clone|
|Synonyms||AREI; PSS4; STF1; STFA|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC203115 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC203115 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||294 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||838 bp|
|RefSeq ORF||297 bp|
|Gene Summary||The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC203115L1||Lenti ORF clone of Human cystatin A (stefin A) (CSTA), Myc-DDK-tagged||
|RC203115L2||Lenti ORF clone of Human cystatin A (stefin A) (CSTA), mGFP tagged||
|RC203115L3||Lenti ORF clone of Human cystatin A (stefin A) (CSTA), Myc-DDK-tagged||
|RC203115L4||Lenti ORF clone of Human cystatin A (stefin A) (CSTA), mGFP tagged||
|RG203115||CSTA (GFP-tagged) - Human cystatin A (stefin A) (CSTA)||
|SC116847||CSTA (untagged)-Human cystatin A (stefin A) (CSTA)||