CLINT1 (NM_014666) Human Tagged ORF Clone

CAT#: RC202387

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CLINT1 (Myc-DDK-tagged)-Human clathrin interactor 1 (CLINT1), transcript variant 2



  "NM_014666" in other vectors (6)

Reconstitution Protocol

USD 582.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CLINT1
Synonyms CLINT; ENTH; EPN4; EPNR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202387 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAACATGTGGAAGGTGCGCGAGCTGGTGGACAAAGCCACCAATGTTGTTATGAATTATTCAGAGA
TCGAGTCTAAGGTTCGAGAGGCAACGAACGATGATCCTTGGGGACCTTCTGGGCAACTCATGGGAGAGAT
TGCCAAGGCTACATTTATGTATGAACAATTTCCAGAACTTATGAACATGCTTTGGTCACGAATGTTAAAA
GACAACAAAAAGAATTGGAGAAGAGTTTATAAGTCGTTGCTGCTCCTAGCTTACCTCATAAGGAATGGAT
CAGAGCGTGTTGTTACAAGTGCCAGAGAACACATTTATGATTTACGATCCCTGGAAAATTACCACTTTGT
AGATGAGCATGGTAAGGATCAAGGTATAAATATTCGACAGAAGGTGAAGGAATTGGTTGAATTTGCCCAG
GATGACGACAGGCTTCGTGAAGAGCGAAAGAAAGCAAAGAAGAACAAAGACAAGTATGTTGGGGTTTCCT
CAGACAGTGTTGGAGGATTCAGATACAGTGAAAGATATGATCCTGAGCCCAAATCAAAATGGGATGAGGA
GTGGGATAAAAACAAGAGTGCTTTTCCATTCAGTGATAAATTAGGTGAGCTGAGTGATAAAATTGGAAGC
ACAATTGATGACACCATCAGCAAGTTCCGGAGGAAAGATAGAGAAGACTCTCCAGAAAGATGCAGCGACA
GCGATGAGGAAAAGAAAGCGAGAAGAGGCAGATCTCCCAAAGGTGAATTCAAAGATGAAGAGGAGACTGT
GACGACAAAGCATATTCATATCACACAGGCCACAGAGACCACCACAACCAGACACAAGCGCACAGCAAAT
CCTTCCAAAACCATTGATCTTGGAGCAGCAGCACATTACACAGGGGACAAAGCAAGTCCAGATCAGAATG
CTTCAACCCACACACCTCAGTCTTCAGTTAAGACTTCAGTGCCTAGCAGCAAGTCATCTGGTGACCTTGT
TGATCTGTTTGATGGCACCAGCCAGTCAACAGGAGGATCAGCTGATTTATTCGGAGGATTTGCTGACTTT
GGCTCAGCTGCTGCATCAGGCAGTTTCCCTTCCCAAGTAACAGCAACAAGTGGGAATGGAGACTTTGGTG
ACTGGAGTGCCTTCAACCAAGCCCCATCAGGCCCTGTTGCTTCCAGTGGCGAGTTCTTTGGCAGTGCCTC
ACAGCCAGCGGTAGAACTTGTTAGTGGCTCACAATCAGCTCTAGGCCCACCTCCTGCTGCCTCAAATTCT
TCAGACCTGTTTGATCTTATGGGCTCGTCCCAGGCAACCATGACATCTTCCCAGAGTATGAATTTCTCTA
TGATGAGCACTAACACTGTGGGACTTGGTTTGCCTATGTCAAGATCACAGAATACAGATATGGTCCAGAA
ATCAGTCAGCAAAACCTTGCCCTCTACTTGGTCTGACCCCAGTGTAAACATCAGCCTAGACAACTTACTA
CCTGGTATGCAGCCTTCCAAACCCCAGCAGCCATCACTGAATACAATGATTCAGCAACAGAATATGCAGC
AGCCTATGAATGTGATGACTCAAAGTTTTGGAGCTGTGAACCTCAGTTCTCCATCGAACATGCTTCCTGT
CCGGCCCCAAACTAATGCTTTGATAGGGGGACCCATGCCTATGAGCATGCCCAATGTGATGACTGGCACC
ATGGGAATGGCCCCTCTTGGAAATACTCCGATGATGAACCAGAGCATGATGGGCATGAACATGAACATAG
GGATGTCCGCTGCTGGGATGGGCTTGACAGGCACAATGGGAATGGGCATGCCCAACATAGCCATGACTTC
TGGAACTGTGCAACCCAAGCAAGATGCCTTTGCAAATTTCGCCAATTTTAGCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202387 protein sequence
Red=Cloning site Green=Tags(s)

MLNMWKVRELVDKATNVVMNYSEIESKVREATNDDPWGPSGQLMGEIAKATFMYEQFPELMNMLWSRMLK
DNKKNWRRVYKSLLLLAYLIRNGSERVVTSAREHIYDLRSLENYHFVDEHGKDQGINIRQKVKELVEFAQ
DDDRLREERKKAKKNKDKYVGVSSDSVGGFRYSERYDPEPKSKWDEEWDKNKSAFPFSDKLGELSDKIGS
TIDDTISKFRRKDREDSPERCSDSDEEKKARRGRSPKGEFKDEEETVTTKHIHITQATETTTTRHKRTAN
PSKTIDLGAAAHYTGDKASPDQNASTHTPQSSVKTSVPSSKSSGDLVDLFDGTSQSTGGSADLFGGFADF
GSAAASGSFPSQVTATSGNGDFGDWSAFNQAPSGPVASSGEFFGSASQPAVELVSGSQSALGPPPAASNS
SDLFDLMGSSQATMTSSQSMNFSMMSTNTVGLGLPMSRSQNTDMVQKSVSKTLPSTWSDPSVNISLDNLL
PGMQPSKPQQPSLNTMIQQQNMQQPMNVMTQSFGAVNLSSPSNMLPVRPQTNALIGGPMPMSMPNVMTGT
MGMAPLGNTPMMNQSMMGMNMNIGMSAAGMGLTGTMGMGMPNIAMTSGTVQPKQDAFANFANFSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014666
ORF Size 1875 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014666.4
RefSeq Size 4007 bp
RefSeq ORF 1878 bp
Locus ID 9685
UniProt ID Q14677
Cytogenetics 5q33.3
Domains ENTH
MW 68.3 kDa
Gene Summary This gene encodes a protein with similarity to the epsin family of endocytic adapter proteins. The encoded protein interacts with clathrin, the adapter protein AP-1 and phosphoinositides. This protein may be involved in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in this gene are associated with a susceptibility to schizophrenia and psychotic disorders. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

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