TRIM38 (NM_006355) Human Tagged ORF Clone

CAT#: RC201944

TRIM38 (Myc-DDK-tagged)-Human tripartite motif containing 38 (TRIM38)

Product Images

Circular map for RC201944

• 

  Western blot validation of overexpression lysate (Cat# LY401912) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201944 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM38
• Synonyms: RNF15; RORET
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201944 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAACCACCAGCACCAAGAAGATGATGGAGGAAGCCACCTGCTCCATCTGCCTGAGCCTGATCA
  CGAACCCAGTAAGCATCAACTGTGGACACAGCTACTGCCACTTGTGTATAACAGACTTCTTTAAAAACCC
  AAGCCAAAAGCAACTGAGGCAGGAGACATTCTGCTGTCCCCAGTGTCGGGCTCCATTTCATATGGATAGC
  CTCCGACCCAACAAGCAGCTGGGAAGCCTCATTGAAGCCCTCAAAGAGACGGATCAAGAAATGTCATGTG
  AGGAACACGGAGAGCAGTTCCACCTGTTCTGCGAAGACGAGGGGCAGCTCATCTGCTGGCGCTGTGAGCG
  GGCACCACAGCACAAAGGGCACACCACAGCTCTTGTTGAAGACGTATGCCAGGGCTACAAGGAAAAGCTC
  CAGAAAGCTGTGACAAAACTGAAGCAACTTGAAGACAGATGTACGGAGCAGAAGCTGTCCACAGCAATGC
  GAATAACTAAATGGAAAGAGAAGGTACAGATTCAGAGACAAAAAATCCGGTCTGACTTTAAGAATCTCCA
  GTGTTTCCTACATGAGGAAGAGAAGTCTTATCTCTGGAGGCTGGAGAAAGAAGAACAACAGACTCTGAGT
  AGACTGAGGGACTATGAGGCTGGTCTGGGGCTGAAGAGCAATGAACTCAAGAGCCACATCCTGGAACTGG
  AGGAAAAATGTCAGGGCTCAGCCCAGAAATTGCTGCAGAATGTGAATGACACTTTGAGCAGGAGTTGGGC
  TGTGAAGCTGGAAACATCAGAGGCTGTCTCCTTGGAACTTCATACTATGTGCAATGTTTCCAAGCTTTAC
  TTCGATGTGAAGAAAATGTTAAGGAGTCATCAAGTTAGTGTGACTCTGGATCCAGATACAGCTCATCACG
  AACTAATTCTCTCTGAGGATCGGAGACAAGTGACTCGTGGATACACCCAGGAGAATCAGGACACATCTTC
  CAGGAGATTTACTGCCTTCCCCTGTGTCTTGGGTTGTGAAGGCTTCACCTCAGGAAGACGTTACTTTGAA
  GTGGATGTTGGCGAAGGAACCGGATGGGATTTAGGAGTTTGTATGGAAAATGTGCAGAGGGGCACTGGCA
  TGAAGCAAGAGCCTCAGTCTGGATTCTGGACCCTCAGGCTGTGCAAAAAGAAAGGCTATGTAGCACTTAC
  TTCTCCCCCAACTTCCCTTCATCTGCATGAGCAGCCCCTGCTTGTGGGAATTTTTCTGGACTATGAGGCC
  GGAGTTGTATCCTTTTATAACGGGAATACTGGCTGCCACATCTTTACTTTCCCGAAGGCTTCCTTCTCTG
  ATACTCTCCGGCCCTATTTCCAGGTTTATCAATATTCTCCTTTGTTTCTGCCTCCCCCAGGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201944 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASTTSTKKMMEEATCSICLSLITNPVSINCGHSYCHLCITDFFKNPSQKQLRQETFCCPQCRAPFHMDS
  LRPNKQLGSLIEALKETDQEMSCEEHGEQFHLFCEDEGQLICWRCERAPQHKGHTTALVEDVCQGYKEKL
  QKAVTKLKQLEDRCTEQKLSTAMRITKWKEKVQIQRQKIRSDFKNLQCFLHEEEKSYLWRLEKEEQQTLS
  RLRDYEAGLGLKSNELKSHILELEEKCQGSAQKLLQNVNDTLSRSWAVKLETSEAVSLELHTMCNVSKLY
  FDVKKMLRSHQVSVTLDPDTAHHELILSEDRRQVTRGYTQENQDTSSRRFTAFPCVLGCEGFTSGRRYFE
  VDVGEGTGWDLGVCMENVQRGTGMKQEPQSGFWTLRLCKKKGYVALTSPPTSLHLHEQPLLVGIFLDYEA
  GVVSFYNGNTGCHIFTFPKASFSDTLRPYFQVYQYSPLFLPPPGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006355
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006355.5
• RefSeq Size: 5629 bp
• RefSeq ORF: 1398 bp
• Locus ID: 10475
• UniProt ID: O00635
• Cytogenetics: 6p22.2
• Domains: zf-B_box, RING, SPRY, PRY
• Protein Families: Druggable Genome
• MW: 53.4 kDa
• Gene Summary: This gene encodes a member of the tripartite motif (TRIM) family. The encoded protein contains a RING-type zinc finger, B box-type zinc finger and SPRY domain. The function of this protein has not been identified. A pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Jul 2012]