RAB23 (NM_016277) Human Tagged ORF Clone
RAB23 (Myc-DDK-tagged)-Human RAB23, member RAS oncogene family (RAB23), transcript variant 1
|Product Name||RAB23 (NM_016277) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC201922 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201922 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||711 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_016277.1, NM_016277.2, NM_016277.3, NM_016277.4, NP_057361.3|
|RefSeq Size||4837 bp|
|RefSeq ORF||714 bp|
|Domains||ras, RAN, RAS, RHO, RAB|
|Protein Families||Druggable Genome|
|Protein Pathways||Hedgehog signaling pathway|
|Gene Summary||This gene encodes a small GTPase of the Ras superfamily. Rab proteins are involved in the regulation of diverse cellular functions associated with intracellular membrane trafficking, including autophagy and immune response to bacterial infection. The encoded protein may play a role in central nervous system development by antagonizing sonic hedgehog signaling. Disruption of this gene has been implicated in Carpenter syndrome as well as cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]|
|The use of this cDNA Clones has been cited in the following citations:|
Rab23 is overexpressed in human bladder cancer and promotes cancer cell proliferation and invasion
,Jiang, Y;Han, Y;Sun, C;Han, C;Han, N;Zhi, W;Qiao, Q;,
Tumour Biol. Dec 2015
,PubMed ID 26715272
|cDNA Clone Resources|
|SC110461||RAB23 (untagged)-Human RAB23, member RAS oncogene family (RAB23), transcript variant 1||
|RG201922||RAB23 (GFP-tagged) - Human RAB23, member RAS oncogene family (RAB23), transcript variant 1||
|RC201922L1||Lenti ORF clone of Human RAB23, member RAS oncogene family (RAB23), transcript variant 1, Myc-DDK-tagged||
|RC201922L2||Lenti ORF clone of Human RAB23, member RAS oncogene family (RAB23), transcript variant 1, mGFP tagged||
|RC201922L3||Lenti ORF clone of Human RAB23, member RAS oncogene family (RAB23), transcript variant 1, Myc-DDK-tagged||
|RC201922L4||Lenti ORF clone of Human RAB23, member RAS oncogene family (RAB23), transcript variant 1, mGFP tagged||