ASB9 (NM_001031739) Human Tagged ORF Clone

CAT#: RC201901

ASB9 (Myc-DDK-tagged)-Human ankyrin repeat and SOCS box containing 9 (ASB9), transcript variant 1

Product Images

Circular map for RC201901

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  Western blot validation of overexpression lysate (Cat# LY422184) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201901 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ASB9 protein (Cat# TP301901). The protein was produced from HEK293T cells transfected with ASB9 cDNA clone (Cat# RC201901) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASB9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201901 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCAAACAAGGGGGCATGGATGGGAGCAAGCCCGCGGGGCCAAGGGACTTTCCTGGCATCAGGC
  TTCTTTCAAACCCATTGATGGGCGATGCTGTGTCTGATTGGTCTCCTATGCATGAAGCTGCAATCCACGG
  ACATCAGCTGTCTCTGAGGAACCTCATCAGCCAGGGGTGGGCTGTGAACATCATCACGGCAGATCATGTT
  TCCCCACTCCATGAAGCCTGTCTTGGAGGTCATCTCTCTTGTGTGAAGATTTTATTAAAGCATGGAGCTC
  AGGTGAATGGTGTGACAGCAGACTGGCACACTCCACTGTTTAATGCTTGTGTCAGCGGCAGCTGGGATTG
  TGTGAATTTGCTTCTGCAGCACGGAGCCAGCGTTCAACCTGAGAGTGATCTGGCATCCCCCATCCATGAA
  GCTGCTAGGAGAGGCCACGTGGAGTGTGTCAACTCTCTTATAGCTTATGGGGGCAACATTGACCATAAGA
  TCAGCCACCTGGGCACTCCACTCTATTTGGCTTGTGAAAACCAACAGAGAGCCTGTGTCAAGAAGCTTCT
  GGAGTCAGGAGCGGACGTGAACCAAGGGAAAGGTCAGGATTCCCCACTTCATGCAGTGGCCAGGACAGCC
  AGTGAAGAGCTGGCCTGCCTGCTCATGGATTTTGGAGCGGACACCCAGGCCAAGAATGCTGAAGGCAAAC
  GTCCTGTGGAGCTGGTGCCTCCAGAGAGCCCCTTGGCCCAGCTCTTCTTGGAGAGAGAAGGGCCCCCTTC
  TTTGATGCAGTTATGCCGCCTTAGAATTCGGAAGTGTTTTGGAATCCAGCAGCATCATAAGATAACCAAA
  CTCGTCCTCCCAGAGGATCTGAAACAGTTTCTCCTACATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201901 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDGKQGGMDGSKPAGPRDFPGIRLLSNPLMGDAVSDWSPMHEAAIHGHQLSLRNLISQGWAVNIITADHV
  SPLHEACLGGHLSCVKILLKHGAQVNGVTADWHTPLFNACVSGSWDCVNLLLQHGASVQPESDLASPIHE
  AARRGHVECVNSLIAYGGNIDHKISHLGTPLYLACENQQRACVKKLLESGADVNQGKGQDSPLHAVARTA
  SEELACLLMDFGADTQAKNAEGKRPVELVPPESPLAQLFLEREGPPSLMQLCRLRIRKCFGIQQHHKITK
  LVLPEDLKQFLLHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031739
• ORF Size: 882 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031739.3
• RefSeq Size: 1714 bp
• RefSeq ORF: 885 bp
• Locus ID: 140462
• UniProt ID: Q96DX5
• Cytogenetics: Xp22.2
• Protein Families: Druggable Genome
• MW: 31.9 kDa
• Gene Summary: This gene encodes a member of the ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein family. Members of this family can interact with the elongin B-C adapter complex via their SOCS box domain and further complex with the cullin and ring box proteins to form E3 ubiquitin ligase complexes. They may function to mediate the substrate-recognition of the E3 ubiquitin ligases. A transcribed pseudogene of this gene has been identified on chromosome 15. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]