DDX41 (NM_016222) Human Tagged ORF Clone

CAT#: RC201898

Reviews ()
Write a review

DDX41 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41)



  "NM_016222" in other vectors (7)

Reconstitution Protocol

USD 868.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "DDX41"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DDX41
Synonyms ABS; MPLPF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201898 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGTCGGAACCCGAACGGAAGCGGGCTCGCACCGACGAGGTGCCTGCCGGAGGAAGCCGCTCCG
AGGCGGAAGATGAGGACGACGAGGACTACGTGCCCTATGTGCCGTTACGGCAGCGCCGGCAGCTACTGCT
CCAGAAGCTGCTGCAGCGAAGACGCAAGGGAGCTGCGGAGGAAGAGCAGCAGGACAGCGGTAGTGAACCC
CGGGGAGATGAGGACGACATCCCGCTAGGCCCTCAGTCCAACGTCAGCCTCCTGGATCAGCACCAGCACC
TTAAAGAGAAGGCTGAAGCGCGCAAAGAGTCTGCCAAGGAGAAGCAGCTGAAGGAAGAAGAGAAGATCCT
GGAGAGTGTTGCCGAGGGCCGAGCATTGATGTCAGTGAAGGAGATGGCTAAGGGCATTACGTATGATGAC
CCCATCAAAACCAGCTGGACTCCACCCCGTTATGTTCTGAGCATGTCTGAAGAGCGACATGAGCGCGTGC
GGAAGAAATACCACATCCTGGTGGAGGGAGACGGTATCCCACCACCCATCAAGAGCTTCAAGGAAATGAA
GTTTCCTGCAGCCATCCTGAGAGGCCTGAAGAAGAAAGGCATTCACCACCCAACACCCATTCAGATCCAG
GGCATCCCCACCATTCTATCTGGCCGTGACATGATAGGCATCGCTTTCACGGGTTCAGGCAAGACACTGG
TGTTCACGTTGCCCGTCATCATGTTCTGCCTGGAACAAGAGAAGAGGTTACCCTTCTCAAAGCGCGAGGG
GCCCTATGGACTCATCATCTGCCCCTCGCGGGAGCTGGCCCGGCAGACCCATGGCATCCTGGAGTACTAC
TGCCGCCTGCTGCAGGAGGACAGCTCACCACTCCTGCGCTGCGCCCTCTGCATTGGGGGCATGTCCGTGA
AAGAGCAGATGGAGACCATCCGACACGGTGTACACATGATGGTGGCCACCCCGGGGCGCCTCATGGATTT
GCTGCAGAAGAAGATGGTCAGCCTAGACATCTGTCGCTACCTGGCCCTGGACGAGGCTGACCGCATGATC
GACATGGGCTTCGAGGGTGACATCCGTACCATCTTCTCCTACTTCAAGGGCCAGCGACAGACCCTGCTCT
TCAGTGCCACCATGCCGAAGAAGATTCAGAACTTTGCTAAGAGTGCCCTTGTAAAGCCTGTGACCATCAA
TGTGGGGCGCGCTGGGGCTGCCAGCCTGGATGTCATCCAGGAGGTAGAATATGTGAAGGAGGAGGCCAAG
ATGGTGTACCTGCTCGAGTGCCTGCAGAAGACACCCCCGCCTGTACTCATCTTTGCAGAGAAGAAGGCAG
ACGTGGACGCCATCCACGAGTACCTGCTGCTCAAGGGGGTTGAGGCCGTAGCCATCCATGGGGGCAAAGA
CCAGGAGGAACGGACTAAGGCCATCGAGGCATTCCGGGAGGGCAAGAAGGATGTCCTAGTAGCCACAGAC
GTTGCCTCCAAGGGCCTGGACTTCCCTGCCATCCAGCACGTCATCAATTATGACATGCCAGAGGAGATTG
AGAACTATGTACACCGGATTGGCCGCACCGGGCGCTCGGGAAACACAGGCATCGCCACTACCTTCATCAA
CAAAGCGTGTGATGAGTCAGTGCTGATGGACCTCAAAGCGCTGCTGCTAGAAGCCAAGCAGAAGGTGCCG
CCCGTGCTGCAGGTGCTGCATTGCGGGGATGAGTCCATGCTGGACATTGGAGGAGAGCGCGGCTGTGCCT
TCTGCGGGGGCCTGGGTCATCGGATCACTGACTGCCCCAAACTCGAGGCTATGCAGACCAAGCAGGTCAG
CAACATCGGTCGCAAGGACTACCTGGCCCACAGCTCCATGGACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201898 protein sequence
Red=Cloning site Green=Tags(s)

MEESEPERKRARTDEVPAGGSRSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEP
RGDEDDIPLGPQSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDD
PIKTSWTPPRYVLSMSEERHERVRKKYHILVEGDGIPPPIKSFKEMKFPAAILRGLKKKGIHHPTPIQIQ
GIPTILSGRDMIGIAFTGSGKTLVFTLPVIMFCLEQEKRLPFSKREGPYGLIICPSRELARQTHGILEYY
CRLLQEDSSPLLRCALCIGGMSVKEQMETIRHGVHMMVATPGRLMDLLQKKMVSLDICRYLALDEADRMI
DMGFEGDIRTIFSYFKGQRQTLLFSATMPKKIQNFAKSALVKPVTINVGRAGAASLDVIQEVEYVKEEAK
MVYLLECLQKTPPPVLIFAEKKADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFREGKKDVLVATD
VASKGLDFPAIQHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVP
PVLQVLHCGDESMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDYLAHSSMDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016222
ORF Size 1866 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016222.4
RefSeq Size 2118 bp
RefSeq ORF 1869 bp
Locus ID 51428
UniProt ID Q9UJV9
Cytogenetics 5q35.3
Domains DEAD, helicase_C, zf-CCHC
Protein Families Druggable Genome
MW 69.8 kDa
Gene Summary DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response. [provided by RefSeq, Jan 2017]

Other Versions

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.