GNPAT (NM_014236) Human Tagged ORF Clone

CAT#: RC201775

GNPAT (Myc-DDK-tagged)-Human glyceronephosphate O-acyltransferase (GNPAT)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GNPAT (Cat# RC201775, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNPAT (Cat# TA811144)(1:2000). Positive lysates LY402292 (100ug) and LC402292 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY402292) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201775 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNPAT
• Synonyms: DAP-AT; DAPAT; DHAPAT; RCDP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201775 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTTCCAGTTCATCTAACTCTTATTTCTCCGTTGGCCCAACCAGTCCCAGCGCTGTCGTGCTCC
  TCTACTCGAAGGAGCTCAAAAAGTGGGATGAGTTTGAAGATATTTTAGAAGAGAGGAGGCATGTCAGTGA
  CTTGAAATTTGCAATGAAATGCTACACACCTCTTGTCTATAAGGGAATTACTCCATGTAAACCAATTGAT
  ATTAAATGTAGTGTTCTCAATTCTGAGGAGATTCATTATGTCATTAAACAGCTTTCCAAGGAATCCCTTC
  AATCTGTGGATGTCCTCCGAGAGGAAGTGAGTGAGATCTTAGATGAAATGAGTCACAAACTGCGTCTTGG
  AGCCATTCGGTTTTGTGCCTTCACCCTGAGCAAAGTATTTAAACAAATTTTCTCGAAGGTGTGTGTAAAT
  GAAGAAGGTATTCAGAAACTACAAAGAGCCATCCAGGAGCATCCTGTTGTTCTGCTGCCTAGTCATCGAA
  GTTACATTGACTTCCTCATGTTGTCTTTTCTTCTATACAATTATGATTTGCCTGTGCCAGTTATAGCAGC
  AGGAATGGACTTCCTGGGAATGAAAATGGTTGGTGAGCTGCTACGAATGTCGGGTGCCTTTTTCATGCGG
  CGTACCTTTGGTGGCAATAAACTCTACTGGGCTGTATTCTCTGAATATGTAAAAACTATGTTACGGAATG
  GTTATGCTCCTGTTGAATTTTTCCTCGAAGGGACAAGAAGCCGCTCTGCCAAGACATTGACTCCTAAATT
  TGGTCTTCTGAATATTGTGATGGAGCCATTTTTTAAAAGAGAAGTTTTTGATACCTACCTTGTCCCAATT
  AGTATCAGTTATGATAAGATCTTGGAAGAAACTCTTTATGTGTATGAGCTTCTAGGGGTTCCTAAACCAA
  AAGAATCTACAACTGGGTTGCTGAAAGCCAGAAAGATTCTCTCTGAAAATTTTGGAAGCATCCATGTGTA
  CTTTGGAGATCCTGTGTCACTTCGATCTTTGGCAGCTGGGAGGATGAGTCGGAGCTCATATAACTTGGTT
  CCAAGATACATTCCTCAGAAACAGTCTGAGGACATGCATGCCTTTGTCACTGAAGTTGCCTACAAAATGG
  AGCTTCTGCAAATTGAAAACATGGTTTTGAGCCCCTGGACCCTAATAGTTGCTGTTCTGCTTCAGAACCG
  GCCATCCATGGACTTTGATGCTCTGGTGGAAAAGACTTTATGGCTAAAAGGCTTAACCCAGGCATTTGGA
  GGGTTTCTCATTTGGCCTGATAATAAACCTGCTGAAGAAGTTGTCCCGGCCAGCATTCTTCTGCATTCCA
  ACATTGCCAGCCTTGTCAAAGACCAGGTGATTCTGAAAGTGGACTCCGGAGACTCGGAAGTGGTCGATGG
  GCTTATGCTCCAGCACATCACTCTCCTCATGTGCTCAGCTTATAGGAACCAGCTGCTCAACATTTTTGTG
  CGCCCATCCTTAGTAGCAGTAGCATTGCAGATGACACCAGGGTTCAGGAAAGAGGATGTCTACAGTTGCT
  TTCGCTTCCTACGTGATGTTTTTGCAGATGAGTTCATCTTCCTTCCAGGAAACACACTAAAGGACTTTGA
  AGAAGGCTGTTACCTGCTTTGTAAAAGTGAAGCCATACAAGTGACTACGAAAGACATCCTAGTTACAGAG
  AAAGGAAATACTGTGTTAGAATTTTTAGTAGGACTCTTTAAACCTTTTGTGGAAAGCTATCAGATAATTT
  GCAAGCACCTTTTGAGTGAAGAAGAGGACCACTTCAGTGAGGAACAGTACTTGGCTGCAGTCAGAAAATT
  CACAAGTCAGCTTCTCGATCAAGGTACCTCTCAATGTTATGATGTATTATCTTCTGATGTGCAGAAAAAC
  GCCTTAGCAGCCTGTGTGAGGCTCGGAGTAGTGGAGAAGAAGAAGATAAATAATAACTGTATATTTAATG
  TGAATGAACCTGCCACAACCAAATTAGAAGAAATGCTTGGTTGTAAGACACCAATAGGAAAACCAGCCAC
  TGCAAAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201775 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESSSSSNSYFSVGPTSPSAVVLLYSKELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPID
  IKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVN
  EEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMR
  RTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPI
  SISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLV
  PRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFG
  GFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFV
  RPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTE
  KGNTVLEFLVGLFKPFVESYQIICKHLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKN
  ALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014236
• ORF Size: 2040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014236.4
• RefSeq Size: 2687 bp
• RefSeq ORF: 2043 bp
• Locus ID: 8443
• UniProt ID: O15228
• Cytogenetics: 1q42.2
• Domains: Acyltransferase
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism
• MW: 77.2 kDa
• Gene Summary: This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]