GNPAT (NM_014236) Human Tagged ORF Clone

CAT#: RC201775

GNPAT (Myc-DDK-tagged)-Human glyceronephosphate O-acyltransferase (GNPAT)

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USD 577.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name GNPAT (NM_014236) Human Tagged ORF Clone
Symbol GNPAT
Synonyms DAP-AT; DAPAT; DHAPAT
Vector pCMV6-Entry
Sequence Data
ORF Nucleotide Sequence
>RC201775 representing NM_014236
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCTTCCAGTTCATCTAACTCTTATTTCTCCGTTGGCCCAACCAGTCCCAGCGCTGTCGTGCTCC
TCTACTCGAAGGAGCTCAAAAAGTGGGATGAGTTTGAAGATATTTTAGAAGAGAGGAGGCATGTCAGTGA
CTTGAAATTTGCAATGAAATGCTACACACCTCTTGTCTATAAGGGAATTACTCCATGTAAACCAATTGAT
ATTAAATGTAGTGTTCTCAATTCTGAGGAGATTCATTATGTCATTAAACAGCTTTCCAAGGAATCCCTTC
AATCTGTGGATGTCCTCCGAGAGGAAGTGAGTGAGATCTTAGATGAAATGAGTCACAAACTGCGTCTTGG
AGCCATTCGGTTTTGTGCCTTCACCCTGAGCAAAGTATTTAAACAAATTTTCTCGAAGGTGTGTGTAAAT
GAAGAAGGTATTCAGAAACTACAAAGAGCCATCCAGGAGCATCCTGTTGTTCTGCTGCCTAGTCATCGAA
GTTACATTGACTTCCTCATGTTGTCTTTTCTTCTATACAATTATGATTTGCCTGTGCCAGTTATAGCAGC
AGGAATGGACTTCCTGGGAATGAAAATGGTTGGTGAGCTGCTACGAATGTCGGGTGCCTTTTTCATGCGG
CGTACCTTTGGTGGCAATAAACTCTACTGGGCTGTATTCTCTGAATATGTAAAAACTATGTTACGGAATG
GTTATGCTCCTGTTGAATTTTTCCTCGAAGGGACAAGAAGCCGCTCTGCCAAGACATTGACTCCTAAATT
TGGTCTTCTGAATATTGTGATGGAGCCATTTTTTAAAAGAGAAGTTTTTGATACCTACCTTGTCCCAATT
AGTATCAGTTATGATAAGATCTTGGAAGAAACTCTTTATGTGTATGAGCTTCTAGGGGTTCCTAAACCAA
AAGAATCTACAACTGGGTTGCTGAAAGCCAGAAAGATTCTCTCTGAAAATTTTGGAAGCATCCATGTGTA
CTTTGGAGATCCTGTGTCACTTCGATCTTTGGCAGCTGGGAGGATGAGTCGGAGCTCATATAACTTGGTT
CCAAGATACATTCCTCAGAAACAGTCTGAGGACATGCATGCCTTTGTCACTGAAGTTGCCTACAAAATGG
AGCTTCTGCAAATTGAAAACATGGTTTTGAGCCCCTGGACCCTAATAGTTGCTGTTCTGCTTCAGAACCG
GCCATCCATGGACTTTGATGCTCTGGTGGAAAAGACTTTATGGCTAAAAGGCTTAACCCAGGCATTTGGA
GGGTTTCTCATTTGGCCTGATAATAAACCTGCTGAAGAAGTTGTCCCGGCCAGCATTCTTCTGCATTCCA
ACATTGCCAGCCTTGTCAAAGACCAGGTGATTCTGAAAGTGGACTCCGGAGACTCGGAAGTGGTCGATGG
GCTTATGCTCCAGCACATCACTCTCCTCATGTGCTCAGCTTATAGGAACCAGCTGCTCAACATTTTTGTG
CGCCCATCCTTAGTAGCAGTAGCATTGCAGATGACACCAGGGTTCAGGAAAGAGGATGTCTACAGTTGCT
TTCGCTTCCTACGTGATGTTTTTGCAGATGAGTTCATCTTCCTTCCAGGAAACACACTAAAGGACTTTGA
AGAAGGCTGTTACCTGCTTTGTAAAAGTGAAGCCATACAAGTGACTACGAAAGACATCCTAGTTACAGAG
AAAGGAAATACTGTGTTAGAATTTTTAGTAGGACTCTTTAAACCTTTTGTGGAAAGCTATCAGATAATTT
GCAAGCACCTTTTGAGTGAAGAAGAGGACCACTTCAGTGAGGAACAGTACTTGGCTGCAGTCAGAAAATT
CACAAGTCAGCTTCTCGATCAAGGTACCTCTCAATGTTATGATGTATTATCTTCTGATGTGCAGAAAAAC
GCCTTAGCAGCCTGTGTGAGGCTCGGAGTAGTGGAGAAGAAGAAGATAAATAATAACTGTATATTTAATG
TGAATGAACCTGCCACAACCAAATTAGAAGAAATGCTTGGTTGTAAGACACCAATAGGAAAACCAGCCAC
TGCAAAACTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC201775 representing NM_014236
Red=Cloning site Green=Tags(s)

MESSSSSNSYFSVGPTSPSAVVLLYSKELKKWDEFEDILEERRHVSDLKFAMKCYTPLVYKGITPCKPID
IKCSVLNSEEIHYVIKQLSKESLQSVDVLREEVSEILDEMSHKLRLGAIRFCAFTLSKVFKQIFSKVCVN
EEGIQKLQRAIQEHPVVLLPSHRSYIDFLMLSFLLYNYDLPVPVIAAGMDFLGMKMVGELLRMSGAFFMR
RTFGGNKLYWAVFSEYVKTMLRNGYAPVEFFLEGTRSRSAKTLTPKFGLLNIVMEPFFKREVFDTYLVPI
SISYDKILEETLYVYELLGVPKPKESTTGLLKARKILSENFGSIHVYFGDPVSLRSLAAGRMSRSSYNLV
PRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFG
GFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFV
RPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTE
KGNTVLEFLVGLFKPFVESYQIICKHLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKN
ALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_014236
ORF Size 2040 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_014236.1, NM_014236.2, NM_014236.3, NP_055051
RefSeq Size 2431
RefSeq ORF 2043
Locus ID 8443
Cytogenetics 1q42.2
Domains Acyltransferase
Protein Families Druggable Genome
Protein Pathways Glycerophospholipid metabolism
MW 77 kDa
Gene Summary This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
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Citations