GNPAT (NM_014236) Human Untagged Clone

CAT#: SC115106

GNPAT (untagged)-Human glyceronephosphate O-acyltransferase (GNPAT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GNPAT
• Synonyms: DAP-AT; DAPAT; DHAPAT; RCDP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014236 edited
  GAATTCGCACGAGGGGCGCCCGGGATCCTGTGTAGCGGCTGCAGAGGGTGCCGCCGCCCT
  AGGCGAAGTAGGGCCGTCCTGAGCGAAAGAACCGCCCCCAGCAGGAGCACCACCACGGCT
  TAGCAAAGAATCCCAGACCCCGCCCGGGAAGGCAGCCGCACCATGGAGTCTTCCAGTTCA
  TCTAACTCTTATTTCTCCGTTGGCCCAACCAGTCCCAGCGCTGTCGTGCTCCTCTACTCG
  AAGGAGCTCAAAAAGTGGGATGAGTTTGAAGATATTTTAGAAGAGAGGAGGCATGTCAGT
  GACTTGAAATTTGCAATGAAATGCTACACACCTCTTGTCTATAAGGGAATTACTCCATGT
  AAACCAATTGATATTAAATGTAGTGTTCTCAATTCTGAGGAGATTCATTATGTCATTAAA
  CAGCTTTCCAAGGAATCCCTTCAATCTGTGGATGTCCTCCGAGAGGAAGTGAGTGAGATC
  TTAGATGAAATGAGTCACAAACTGCGTCTTGGAGCCATTCGGTTTTGTGCCTTCACCCTG
  AGCAAAGTATTTAAACAAATTTTCTCGAAGGTGTGTGTAAATGAAGAAGGTATTCAGAAA
  CTACAAAGAGCCATCCAGGAGCATCCTGTTGTTCTGCTGCCTAGTCATCGAAGTTACATT
  GACTTCCTCATGTTGTCTTTTCTTCTATACAATTATGATTTGCCTGTGCCAGTTATAGCA
  GCAGGAATGGACTTCCTGGGAATGAAAATGGTTGGTGAGCTGCTACGAATGTCGGGTGCC
  TTTTTCATGCGGCGTACCTTTGGTGGCAATAAACTCTACTGGGCTGTATTCTCTGAATAT
  GTAAAAACTATGTTACGGAATGGTTATGCTCCTGTTGAATTTTTCCTCGAAGGGACAAGA
  AGCCGCTCTGCCAAGACATTGACTCCTAAATTTGGTCTTCTGAATATTGTGATGGAGCCA
  TTTTTTAAAAGAGAAGTTTTTGATACCTACCTTGTCCCAATTAGTATCAGTTATGATAAG
  ATCTTGGAAGAAACTCTTTATGTGTATGAGCTTCTAGGGGTTCCTAAACCAAAAGAGTCT
  ACAACTGGGTTGCTGAAAGCCAGAAAGATTCTCTCTGAAAATTTTGGAAGCATCCATGTG
  TACTTTGGAGATCCTGTGTCACTTCGATCTTTGGCAGCTGGGAGGATGAGTCGGAGCTCA
  TATAACTTGGTTCCAAGATACATTCCTCAGAAACAGTCTGAGGACATGCATGCCTTTGTC
  ACTGAAGTTGCCTACAAAATGGAGCTTCTGCAAATTGAAAACATGGTTTTGAGCCCCTGG
  ACCCTAATAGTTGCTGTTCTGCTTCAGAACCGGCCATCCATGGACTTTGATGCTCTGGTG
  GAAAAGACTTTATGGCTAAAAGGCTTAACCCAGGCATTTGGAGGGTTTCTCATTTGGCCT
  GATAATAAACCTGCTGAAGAAGTTGTCCCGGCCAGCATTCTTCTGCATTCCAACATTGCC
  AGCCTTGTCAAAGACCAGGTGATTCTGAAAGTGGACTCCGGAGACTCGGAAGTGGTCGAT
  GGGCTTATGCTCCAGCACATCACTCTCCTCATGTGCTCAGCTTATAGGAACCAGCTGCTC
  AACATTTTTGTGCGCCCATCCTTAGTAGCAGTAGCATTGCAGATGACACCAGGGTTCAGG
  AAAGAGGATGTCTACAGTTGCTTTCGCTTCCTACGTGATGTTTTTGCAGATGAGTTCATC
  TTCCTTCCAGGAAACACACTAAAGGACTTTGAAGAAGGCTGTTACCTGCTTTGTAAAAGT
  GAAGCCATACAAGTGACTACGAAAGACATCCTAGTTACAGAGAAAGGAAATACTGTGTTA
  GAATTTTTAGTAGGACTCTTTAAACCTTTTGTGGAAAGCTATCAGATAATTTGCAAGTAC
  CTTTTGAGTGAAGAAGAGGACCACTTCAGTGAGGAACAGTACTTGGCTGCAGTCAGAAAA
  TTCACAAGTCAGCTTCTCGATCAAGGTACCTCTCAATGTTATGATGTATTATCTTCTGAT
  GTGCAGAAAAATGCCTTAGCAGCCTGTGTGAGGCTCGGAGTAGTGGAGAAGAAGAAGATA
  AATAATAACTGTATATTTAATGTGAATGAACCTGCCACAACCAAATTAGAAGAAATGCTT
  GGTTGTAAGACACCAATAGGAAAACCAGCCACTGCAAAACTTTAATAATCAACAAATAGT
  TATGGAAAATTCGGTCACGTAATTACTCTCATCGAAGGACTCATTACAACAAACAGGGAA
  GTAAAGGAAGAGACACATCCTCTCATACTCCCTGAGACTCTGAGAACAGTGGACGCAGAG
  GGAAGAGATGATCATTGGAAGCAATCAGTTTACTCTTCCCCACCACAGTGGTTAAAAGGC
  GTTTGTATCTGACACTATGTGTGTGTTTTAAAATAAACTTTTGGAAACATGTTTGGAAAA
  AAAAAAAAAAAAAACTCGATCCTAGATTGCGGCCGCGGG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014236 unedited
  GTTCAAATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGCCCGG
  GATCCTGTGTAGCGGCTGCAGAGGGTGCCGCCGCCCTAGGCGAAGTAGGGCCGTCCTGAG
  CGAAAGAACCGCCCCCAGCAGGAGCACCACCACGGCTTAGCAAAGAATCCCAGACCCCGC
  CCGGGAAGGCAGCCGCACCATGGAGTCTTCCAGTTCATCTAACTCTTATTTCTCCGTTGG
  CCCAACCAGTCCCAGCGCTGTCGTGCTCCTCTACTCGAAGGAGCTCAAAAAGTGGGATGA
  GTTTGAAGATATTTTAGAAGAGAGGAGGCATGTCAGTGACTTGAAATTTGCAATGAAATG
  CTACACACCTCTTGTCTATAAGGGAATTACTCCATGTAAACCAATTGATATTAAATGTAG
  TGTTCTCAATTCTGAGGAGATTCATTATGTCATTAAACAGCTTTCCAAGGAATCCCTTCA
  ATCTGTGGATGTCCTCCGAGAGGAAGTGAGTGAGATCTTAGATGAAATGAGTCACAAACT
  GCGTCTTGGAGCCATTCGGTTNTGTGCCTTCACCCTGAGCAAAGTATTTAAACAAATTTT
  CTCGAAGGTGTGTGTAAATGAAGAAGGTATTCAGAAACTACAAAGAGCCATCCAGGAGCA
  TCCTGTTGTTCTGCTGCCTAGTCATCGAAGTTACATTGACTTCCTCATGTTGTCTTTTCT
  TCTATACAATTATGATTTGCCTGTGCCAGTTATAGCAGCAGGAATGGACTTCCTGGGAAT
  GAAAATGGTTGGTGAGCTGCTACNAATGGTCGGTGCCTTTTTCATGCGGCGTACCTTTGG
  TGGCATAAACTCTACTGGGCTGTATTCTCTGATATGTAAAACTATGTACGGNAATGGTAT
  GCTCCTGTTGAATTTTNCCTCGAAGGGACAAGAGCCGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014236 unedited
  TTAGCTATGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAAAC
  ATGTTTCCAAAGTTTATTTTAAAACACACACATAGTGTCAGATACAAACGCCTTTTAACC
  ACTGTGGTGGGGAAGAGTAAACTGATTGCTTCCAATGATCATCTCTTCCCTCTGCGTCCA
  CTGTTCTCAGAGTCTCAGGGAGTATGAGAGGATGTGTCTCTTCCTTTACTTCCCTGTTTG
  TTGTAATGAGTCCTTCGATGAGAGTAATTACGTGACCGAATTTTCCATAACTATTTGTTG
  ATTATTAAAGTTTTGCAGTGGCTGGTTTTCCTATTGGTGTCTTACAACCAAGCATTTCTT
  CTAATTTGGTTGTGGCAGGTTCATTCACATTAAATATACAGTTATTATTTATCTTCTTCT
  TCTCCACTACTCCGAGCCTCACACAGGCTGCTAAGGCATTTTTCTGCACATCAGAAGATA
  ATACATCATAACATTGAGAGGTACCTTGATCGAGAAGCTGACTTGTGAATTTTCTGACTG
  CAGCCAAGTACTGTTCCTCACTGAAGTGGTCCTCTTCTTCACTCAAAAGGTACTTGCAAA
  TTATCTGATAGCTTTCCACAAAAGGTTTAAAGAGTCCTACTAAAAATTCTAACACAGTAT
  TTCCTTTCTCTGTAACTAGGATGTCTTTCGTAGTCACTTGTATGGCTTCACTTTTACAAA
  GCAGGTAACAGCCTTCTTCAAAGTCCTTTAATGTGTTTCCTGGAAGGAAGATGAACTCAT
  CTGCAAAAACTCACGTTAGAAGCGAAACAACTGTAGACTCCTCTTTCCTGACCCTGGTGT
  CATCTGCATGCTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014236
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014236.2, NP_055051.1
• RefSeq Size: 2431 bp
• RefSeq ORF: 2043 bp
• Locus ID: 8443
• UniProt ID: O15228
• Cytogenetics: 1q42.2
• Domains: Acyltransferase
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).