NM23A (NME1) (NM_000269) Human Tagged ORF Clone
NME1 (Myc-DDK-tagged)-Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 2
|Type||Human Tagged ORF Clone|
|Synonyms||AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC201731 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201731 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||456 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||811 bp|
|RefSeq ORF||459 bp|
|Protein Families||Druggable Genome, Stem cell - Pluripotency|
|Protein Pathways||Metabolic pathways, Purine metabolism, Pyrimidine metabolism|
|Gene Summary||This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC201731L1||Lenti ORF clone of Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 2, Myc-DDK-tagged||
|RC201731L2||Lenti ORF clone of Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 2, mGFP tagged||
|RC201731L3||Lenti ORF clone of Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 2, Myc-DDK-tagged||
|RC201731L4||Lenti ORF clone of Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 2, mGFP tagged||
|RG201731||NME1 (GFP-tagged) - Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 2||
|SC125467||NME1 (untagged)-Human non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 2||