Fibrillarin (FBL) (NM_001436) Human Tagged ORF Clone
FBL (Myc-DDK-tagged)-Human fibrillarin (FBL)
|Type||Human Tagged ORF Clone|
|Synonyms||FIB; FLRN; Nop1; RNU3IP1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC200732 representing NM_001436
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200732 representing NM_001436
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||963 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1135 bp|
|RefSeq ORF||966 bp|
|Protein Families||Stem cell - Pluripotency|
|Gene Summary||This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC200732L1||Lenti ORF clone of Human fibrillarin (FBL), Myc-DDK-tagged||
|RC200732L2||Lenti ORF clone of Human fibrillarin (FBL), mGFP tagged||
|RC200732L3||Lenti ORF clone of Human fibrillarin (FBL), Myc-DDK-tagged||
|RC200732L4||Lenti ORF clone of Human fibrillarin (FBL), mGFP tagged||
|RG200732||FBL (GFP-tagged) - Human fibrillarin (FBL)||
|SC319339||FBL (untagged)-Human fibrillarin (FBL)||