RNF40 (NM_014771) Human Tagged ORF Clone

CAT#: RC200575

RNF40 (Myc-DDK-tagged)-Human ring finger protein 40 (RNF40), transcript variant 1

Product Images

Circular map for RC200575

• 

  Western blot validation of overexpression lysate (Cat# LY402375) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200575 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF40
• Synonyms: BRE1B; RBP95; STARING
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200575 representing NM_014771
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGGCCAGGCAACAAACGCGCCGCCGGCGACGGGGGCTCAGGGCCCCCGGAAAAGAAGCTGAGTC
  GTGAGGAGAAGACCACCACGACTCTTATCGAGCCCATTCGTCTTGGAGGCATCTCTTCCACGGAGGAGAT
  GGACCTGAAGGTACTACAGTTCAAGAACAAGAAACTGGCAGAGCGGCTGGAACAACGGCAGGCTTGTGAA
  GATGAACTCCGAGAACGAATTGAGAAGTTGGAGAAGCGGCAGGCCACAGATGATGCCACACTCCTCATCG
  TCAATCGCTACTGGGCCCAGCTGGATGAAACTGTGGAAGCCCTTCTCCGATGCCATGAGAGCCAGGGGGA
  GCTGTCTTCAGCGCCTGAGGCACCTGGGACCCAGGAGGGGCCAACATGTGATGGGACTCCTCTCCCAGAG
  CCGGGGACATCAGAGCTGAGAGACCCCTTGCTGATGCAGCTGCGGCCCCCTCTCAGTGAGCCGGCCTTGG
  CTTTTGTGGTGGCACTGGGTGCCAGCAGCAGTGAGGAGGTGGAGCTGGAGCTGCAAGGCCGAATGGAGTT
  CTCCAAGGCAGCTGTGTCTCGTGTGGTAGAGGCCTCAGACCGCCTACAGCGCCGGGTGGAGGAACTCTGT
  CAGCGAGTGTACAGCCGAGGGGACAGTGAGCCCCTCAGTGAGGCGGCTCAGGCACACACCCGAGAGCTGG
  GCCGTGAGAACCGGCGACTGCAGGACTTGGCCACTCAGCTGCAGGAGAAACACCACCGCATCTCATTGGA
  GTACTCCGAGCTCCAGGATAAAGTGACATCGGCAGAGACCAAGGTGCTGGAGATGGAGACAACAGTGGAG
  GACTTGCAGTGGGACATCGAGAAGCTGCGGAAGCGAGAGCAAAAGCTCAATAAGCACCTGGCAGAGGCCT
  TAGAGCAGCTTAACTCTGGCTACTATGTATCTGGGAGCTCCTCAGGCTTCCAGGGGGGCCAGATCACACT
  CAGCATGCAGAAGTTTGAGATGCTGAATGCAGAGTTAGAGGAAAACCAGGAACTGGCCAACAGCCGTATG
  GCAGAGCTGGAGAAACTGCAGGCCGAACTTCAGGGGGCTGTGCGGACCAATGAGCGCCTCAAGGTGGCCC
  TGCGGAGCCTTCCTGAGGAGGTAGTGCGGGAGACGGGGGAGTACCGCATGCTGCAGGCCCAATTCTCACT
  GCTCTACAACGAGTCTCTGCAAGTGAAGACCCAGCTAGACGAGGCTCGGGGCCTGCTGCTGGCCACAAAG
  AACTCCCACCTGCGACACATCGAGCACATGGAGAGCGACGAGCTGGGGCTGCAGAAGAAGCTACGCACAG
  AGGTCATTCAGCTGGAGGACACGCTGGCCCAGGTACGCAAGGAGTATGAGATGCTGCGCATCGAGTTTGA
  GCAGAATCTGGCGGCCAACGAGCAGGCGGGGCCCATCAACCGTGAGATGCGCCACCTGATTAGTAGTCTT
  CAAAACCACAACCACCAGCTAAAAGGGGACGCCCAGCGATACAAGCGGAAGCTTCGAGAAGTACAAGCTG
  AGATTGGCAAGCTCCGGGCCCAGGCCAGTGGCTCTGCCCACTCCACCCCCAACCTGGGCCACCCAGAGGA
  TTCTGGCGTCAGTGCCCCAGCCCCAGGGAAAGAGGAGGGTGGGCCAGGCCCTGTCAGTACCCCCGACAAC
  AGAAAGGAGATGGCTCCAGTGCCTGGCACCACCACTACTACCACTTCAGTGAAGAAGGAGGAGCTGGTCC
  CCTCTGAAGAGGACTTCCAGGGTATAACCCCTGGGGCCCAGGGCCCTTCCTCCCGGGGCCGAGAACCTGA
  GGCCAGGCCCAAGCGGGAGCTTCGGGAACGGGAAGGTCCCAGCCTAGGACCTCCACCTGTAGCCTCCGCT
  CTCTCAAGGGCTGATCGGGAGAAGGCCAAGGTGGAAGAAACCAAGCGGAAGGAATCAGAACTCCTCAAGG
  GTCTCCGAGCAGAGCTCAAGAAGGCCCAGGAGAGCCAGAAGGAGATGAAACTGCTGCTGGATATGTACAA
  GTCAGCGCCCAAGGAGCAGCGGGATAAGGTGCAGCTCATGGCAGCGGAACGCAAGGCTAAGGCCGAGGTT
  GATGAGCTGCGGAGCCGCATCCGGGAATTGGAGGAGAGGGATCGAAGGGAGAGCAAGAAGATCGCGGATG
  AGGATGCCCTGCGGCGCATTCGGCAGGCAGAGGAGCAGATAGAACACCTGCAGCGCAAGCTGGGTGCCAC
  CAAGCAGGAGGAGGAGGCTCTGCTCTCAGAGATGGATGTGACAGGTCAGGCTTTTGAGGACATGCAGGAA
  CAGAACGGGCGGCTGCTACAGCAGTTGCGGGAAAAGGATGATGCCAACTTTAAGCTAATGTCAGAGCGGA
  TCAAGGCCAACCAGATTCACAAGCTGCTGCGGGAGGAGAAGGATGAGTTGGGCGAGCAGGTCCTTGGCCT
  CAAGTCCCAGGTGGATGCCCAGCTGCTGACTGTGCAGAAGCTGGAGGAGAAGGAGCGAGCCTTGCAGGGC
  AGCCTCGGGGGTGTGGAGAAGGAGCTGACGCTGCGCAGCCAAGCCCTGGAGCTCAACAAGCGGAAGGCTG
  TAGAAGCCGCCCAGCTGGCCGAGGACCTGAAGGTGCAGCTGGAGCACGTGCAGACTCGGCTGCGGGAGAT
  CCAGCCCTGCCTGGCAGAGAGCCGGGCTGCTCGTGAGAAAGAGAGCTTCAACCTCAAGAGGGCTCAGGAG
  GACATCTCACGGCTGCGGCGCAAGCTGGAAAAGCAGAGGAAGGTGGAGGTCTACGCAGATGCCGACGAAA
  TCCTCCAGGAGGAGATCAAGGAGTACAAGGCGCGGTTGACCTGCCCCTGCTGTAACACCCGCAAGAAGGA
  TGCAGTCCTTACCAAGTGCTTCCACGTTTTCTGCTTCGAGTGCGTGCGGGGCCGCTATGAGGCCCGCCAG
  AGGAAGTGCCCCAAGTGCAACGCGGCCTTTGGTGCCCACGACTTCCATCGTATCTACATCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200575 representing NM_014771
  Red=Cloning site Green=Tags(s)
  
  MSGPGNKRAAGDGGSGPPEKKLSREEKTTTTLIEPIRLGGISSTEEMDLKVLQFKNKKLAERLEQRQACE
  DELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHESQGELSSAPEAPGTQEGPTCDGTPLPE
  PGTSELRDPLLMQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSRVVEASDRLQRRVEELC
  QRVYSRGDSEPLSEAAQAHTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVE
  DLQWDIEKLRKREQKLNKHLAEALEQLNSGYYVSGSSSGFQGGQITLSMQKFEMLNAELEENQELANSRM
  AELEKLQAELQGAVRTNERLKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLATK
  NSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSL
  QNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASGSAHSTPNLGHPEDSGVSAPAPGKEEGGPGPVSTPDN
  RKEMAPVPGTTTTTTSVKKEELVPSEEDFQGITPGAQGPSSRGREPEARPKRELREREGPSLGPPPVASA
  LSRADREKAKVEETKRKESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKAKAEV
  DELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQE
  QNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQG
  SLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQE
  DISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQ
  RKCPKCNAAFGAHDFHRIYIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014771
• ORF Size: 3003 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014771.3
• RefSeq Size: 4256 bp
• RefSeq ORF: 3006 bp
• Locus ID: 9810
• UniProt ID: O75150
• Cytogenetics: 16p11.2
• Domains: RING
• Protein Families: Druggable Genome
• MW: 113.5 kDa
• Gene Summary: The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]