RNF40 (NM_014771) Human Untagged Clone

CAT#: SC320843

RNF40 (untagged)-Human ring finger protein 40 (RNF40), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF40
• Synonyms: BRE1B; RBP95; STARING
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014771.2 GCTGACCCTCCTGCTGGTGACGGAAGTACCGCCTCCTCCCGTCTGACGCCCCTCAGGGGA
  CCCTGCATCGCTCCAGCCGCCGCGGCCATGTCTGGGCCAGGCAACAAACGCGCCGCCGGC
  GACGGGGGCTCAGGGCCCCCGGAAAAGAAGCTGAGTCGTGAGGAGAAGACCACCACGACT
  CTTATCGAGCCCATTCGTCTTGGAGGCATCTCTTCCACGGAGGAGATGGACCTGAAGGTA
  CTACAGTTCAAGAACAAGAAACTGGCAGAGCGGCTGGAACAACGGCAGGCTTGTGAAGAT
  GAACTCCGAGAACGAATTGAGAAGTTGGAGAAGCGGCAGGCCACAGATGATGCCACACTC
  CTCATCGTCAATCGCTACTGGGCCCAGCTGGATGAAACTGTGGAAGCCCTTCTCCGATGC
  CATGAGAGCCAGGGGGAGCTGTCTTCAGCGCCTGAGGCACCTGGGACCCAGGAGGGGCCA
  ACATGTGATGGGACTCCTCTCCCAGAGCCGGGGACATCAGAGCTGAGAGACCCCTTGCTG
  ATGCAGCTGCGGCCCCCTCTCAGTGAGCCGGCCTTGGCTTTTGTGGTGGCACTGGGTGCC
  AGCAGCAGTGAGGAGGTGGAGCTGGAGCTGCAAGGCCGAATGGAGTTCTCCAAGGCAGCT
  GTGTCTCGTGTGGTAGAGGCCTCAGACCGCCTACAGCGCCGGGTGGAGGAACTCTGTCAG
  CGAGTGTACAGCCGAGGGGACAGTGAGCCCCTCAGTGAGGCGGCTCAGGCACACACCCGA
  GAGCTGGGCCGTGAGAACCGGCGACTGCAGGACTTGGCCACTCAGCTGCAGGAGAAACAC
  CACCGCATCTCATTGGAGTACTCCGAGCTCCAGGATAAAGTGACATCGGCAGAGACCAAG
  GTGCTGGAGATGGAGACAACAGTGGAGGACTTGCAGTGGGACATCGAGAAGCTGCGGAAG
  CGAGAGCAAAAGCTCAATAAGCACCTGGCAGAGGCCTTAGAGCAGCTTAACTCTGGCTAC
  TATGTATCTGGGAGCTCCTCAGGCTTCCAGGGGGGCCAGATCACACTCAGCATGCAGAAG
  TTTGAGATGCTGAATGCAGAGTTAGAGGAAAACCAGGAACTGGCCAACAGCCGTATGGCA
  GAGCTGGAGAAACTGCAGGCCGAACTTCAGGGGGCTGTGCGGACCAATGAGCGCCTCAAG
  GTGGCCCTGCGGAGCCTTCCTGAGGAGGTAGTGCGGGAGACGGGGGAGTACCGCATGCTG
  CAGGCCCAATTCTCACTGCTCTACAACGAGTCTCTGCAAGTGAAGACCCAGCTAGACGAG
  GCTCGGGGCCTGCTGCTGGCCACAAAGAACTCCCACCTGCGACACATCGAGCACATGGAG
  AGCGACGAGCTGGGGCTGCAGAAGAAGCTACGCACAGAGGTCATTCAGCTGGAGGACACG
  CTGGCCCAGGTACGCAAGGAGTATGAGATGCTGCGCATCGAGTTTGAGCAGAATCTGGCG
  GCCAACGAGCAGGCGGGGCCCATCAACCGTGAGATGCGCCACCTGATTAGTAGTCTTCAA
  AACCACAACCACCAGCTAAAAGGGGACGCCCAGCGATACAAGCGGAAGCTTCGAGAAGTA
  CAAGCTGAGATTGGCAAGCTCCGGGCCCAGGCCAGTGGCTCTGCCCACTCCACCCCCAAC
  CTGGGCCACCCAGAGGATTCTGGCGTCAGTGCCCCAGCCCCAGGGAAAGAGGAGGGTGGG
  CCAGGCCCTGTCAGTACCCCCGACAACAGAAAGGAGATGGCTCCAGTGCCTGGCACCACC
  ACTACTACCACTTCAGTGAAGAAGGAGGAGCTGGTCCCCTCTGAAGAGGACTTCCAGGGT
  ATAACCCCTGGGGCCCAGGGCCCTTCCTCCCGGGGCCGAGAACCTGAGGCCAGGCCCAAG
  CGGGAGCTTCGGGAACGGGAAGGTCCCAGCCTAGGACCTCCACCTGTAGCCTCCGCTCTC
  TCAAGGGCTGATCGGGAGAAGGCCAAGGTGGAAGAAACCAAGCGGAAGGAATCAGAACTC
  CTCAAGGGTCTCCGAGCAGAGCTCAAGAAGGCCCAGGAGAGCCAGAAGGAGATGAAACTG
  CTGCTGGATATGTACAAGTCAGCGCCCAAGGAGCAGCGGGATAAGGTGCAGCTCATGGCA
  GCGGAACGCAAGGCTAAGGCCGAGGTTGATGAGCTGCGGAGCCGCATCCGGGAATTGGAG
  GAGAGGGATCGAAGGGAGAGCAAGAAGATCGCGGATGAGGATGCCCTGCGGCGCATTCGG
  CAGGCAGAGGAGCAGATAGAACACCTGCAGCGCAAGCTGGGTGCCACCAAGCAGGAGGAG
  GAGGCTCTGCTCTCAGAGATGGATGTGACAGGTCAGGCTTTTGAGGACATGCAGGAACAG
  AACGGGCGGCTGCTACAGCAGTTGCGGGAAAAGGATGATGCCAACTTTAAGCTAATGTCA
  GAGCGGATCAAGGCCAACCAGATTCACAAGCTGCTGCGGGAGGAGAAGGATGAGTTGGGC
  GAGCAGGTCCTTGGCCTCAAGTCCCAGGTGGATGCCCAGCTGCTGACTGTGCAGAAGCTG
  GAGGAGAAGGAGCGAGCCTTGCAGGGCAGCCTCGGGGGTGTGGAGAAGGAGCTGACGCTG
  CGCAGCCAAGCCCTGGAGCTCAACAAGCGGAAGGCTGTAGAAGCCGCCCAGCTGGCCGAG
  GACCTGAAGGTGCAGCTGGAGCACGTGCAGACTCGGCTGCGGGAGATCCAGCCCTGCCTG
  GCAGAGAGCCGGGCTGCTCGTGAGAAAGAGAGCTTCAACCTCAAGAGGGCTCAGGAGGAC
  ATCTCACGGCTGCGGCGCAAGCTGGAAAAGCAGAGGAAGGTGGAGGTCTACGCAGATGCC
  GACGAAATCCTCCAGGAGGAGATCAAGGAGTACAAGGCGCGGTTGACCTGCCCCTGCTGT
  AACACCCGCAAGAAGGATGCAGTCCTTACCAAGTGCTTCCACGTTTTCTGCTTCGAGTGC
  GTGCGGGGCCGCTATGAGGCCCGCCAGAGGAAGTGCCCCAAGTGCAACGCGGCCTTTGGT
  GCCCACGACTTCCATCGTATCTACATCAGCTGAACCTGAAACTCAGGGGACTCTGGAACA
  CCATGGACCCTGGGGGCTGTGCCCCCATCTCCTCCCCACCCCAGGTCTAGTGGCCCCACC
  CTCCATTCCGGACCCCATGGGCCCAGCCCCTGCCCATCTAGTTGGTTTGGGGACCCTGGT
  GCATGCTAGTGGGCATGGGATCAGCCAAGCTTCGTTCCATCTTTTCCTAAAGGTCAGAGC
  TGCAGCCTAGGGGGCACTGCCCTACAGAAAAGGTCTGCCTGAGAGGCCTGAGGAGCCCAG
  AGCACTTGACTGAGCTTCCCGGAAACTGGCCCTAACCTGTCTGTCTCCGTGGATGCATCC
  TAACCCTAAGGAAAATTCCCCAGGCTGTGATCTACCCTAGAGAAGGCTCGCTCCCTGCCT
  ACTGGCTCACAAATGAGGACCAGTGAGCCATGTCCTTGTTCCTTGTTTGAGACTGGGCTG
  CAGGCCCCAGGAAGACTTTCCTTCACCCACCATCCCCCTAACCTCGGCAGGGCTTCTGTC
  CTGTGGAGTTCCCTGGACACCTTGGTCTGGCTCTTGTGCCAAGGGCTGAAGGAGGTACCC
  TCTTGGCAGATGGGGGCATCACTTGCTTCCTTTGGGAAGCTCTAAGGTTGCTGCAGTCAC
  CTTCCTCATCTTGCAGGTGCTGAACCAACATCATCAGTTTCTATTCTAATCAGGCCCCTT
  CCCAATCTCCATTTCTCTGCCAAGCCCATTTACCCCCACCTCATGCATCCCAAGGCTCTA
  CTGGGTCCCTGGACCTAACCCTGCTTTCATCCTGGTGGCCTTAACTACAGTGGAGGTGGA
  ACTTCCCAGGAGGGGAAGGGACAGACCAGCCCCAGCCGCTGGGCCAACTTCCAATCATTC
  CAGCTAGAAGAGCTTCCCCCTGACACCCTGTGACTGAGCCTGTGTCCTGTCTGCCTGCCC
  AGCCATGCTCCATCGGCTGTGAGGGCAGTGCCCGGAGAGGCCAGAGGGTTGGAGCTGCAG
  GGACCCGTTTGGACCCACAGCCTCTGTTCTAGAGATGCTTGTATAGGCTGTTAATTGTGA
  TGAATAAACGTTCAACCCTCGGCCTGCAGCCAGAGTAGCCAGGCCGCGCACCCCAAAAAA
  AAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014771
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014771.2, NP_055586.1
• RefSeq Size: 4256 bp
• RefSeq ORF: 3006 bp
• Locus ID: 9810
• UniProt ID: O75150
• Cytogenetics: 16p11.2
• Domains: RING
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.